Antioxidant, Cytotoxic Activities and Metabolite Profile of Flavonoids from Erythrina crista-galli L. Twigs by Ultrasonic Assisted Extraction Method
DOI:
https://doi.org/10.48048/tis.2025.11209Keywords:
Flavonoids; Erythrina crista-galli, Ultrasound-assisted extraction, Antioxidant, Cytotoxic, Metabolite profilingAbstract
Erythrina crista-galli L., a member of the Erythrina genus, is recognized for its high flavonoid content and promising pharmacological potential. This study investigated the impact of different ultrasound-assisted extraction (UAE) condition on the total flavonoid content (TFC) of E. crista-galli twig extract, and evaluated its antioxidant, cytotoxic, and metabolite profiles. Antioxidant activity was determined using the DPPH free radical scavenging assay, while cytotoxicity was assessed against MCF-7, HeLa, and A549 cancer cell lines using the MTT assay. Extraction using UAE with ethanol as a solvent at 40° for 35 min and a mass-to-solvent ratio of 1:25 yielded the highest TFC at 37.69 mg QE/g extract. The extract demonstrated strong antioxidant activity with an IC50 of 54.80 ± 0.26 μg/mL, and moderate cytotoxicity against MCF-7 breast cancer cells (IC50 = 194.6 μg/mL), but showed weak cytotoxicity against HeLa (IC50 = 235.0 μg/mL) and A549 cells (IC50 = 410.3 μg/mL). Comprehensive metabolite profiling using LC-MS/MS identified 34 flavonoid compounds in both positive and negative ion modes, representing the first comprehensive metabolite profile of E. crista-galli twigs. These findings highlight the potential of E. crista-galli as a valuable natural source of bioactive flavonoids for further therapeutic development, including in vivo testing and molecular target identification to validate the efficacy and mechanism of action of these compounds.
HIGHLIGHTS
- This study evaluated the effect of different ultrasound assisted extraction (UAE) condition on total flavonoids content of crista-galli twig extract
- The crista-galli twig extract exhibited strong antioxidant activity against DPPH free radicals with IC50 of 54.80 ± 0.26 μg/mL
- The crista-galli twig extract exhibited moderate cytotoxic activity against MCF-7 breast cancer cell line with IC50 of 194.6 μg/mL, while weak activity against HeLa cervical cancer cell line and A-549 lung cancer cell line with IC50 of 235.0 μg/mL and 410.3 μg/mL respectively.
- To the best of our knowledge, this is the 1st study to report a comprehensive metabolite profiling of flavonoids from Erythrina crista-galli twigs using LC-MS/MS, identifying 34 compounds across both positive and negative ion modes.
- These findings indicate the potential of crista-galli as a natural source of bioactive flavonoid compounds for further therapeutic development.
GRAPHICAL ABSTRACT
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