Impact of Pineapple (Ananas comosus L. Merr) Stem Extract (PSE) on Ovarian Dysfunction in A Polycystic Ovary Syndrome Rat Model: Insights into Molecular Interactions and Biological Pathways
DOI:
https://doi.org/10.48048/tis.2025.11165Keywords:
Ananas comosus L. Merr, Polycystic ovary syndrome (PCOS), Folliculogenesis, Antiandrogenic activity, AGEs, OvulationAbstract
Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder characterized by hyperandrogenism, anovulation, and polycystic ovarian morphology. This study evaluated the therapeutic effects of Pineapple (Ananas comosus L. Merr) stem extract (PSE) in a DHEA-induced PCOS rat model. Thirty-six female Sprague-Dawley rats were randomly divided into 6 groups, including a negative control group. Following 7-day acclimatization and PCOS induction with DHEA, treatments were administered from day 43 (H0) to day 70 (H27). Serum levels of LH, FSH, testosterone, and AGEs were measured by ELISA, while ovarian morphology was assessed through H&E staining. The 500 mg/kg BW PSE treatment significantly reduced LH (p < 0.001), testosterone (p < 0.001), and AGEs (p < 0.001) levels while increasing FSH (p = 0.017). Furthermore, histopathological examination using hematoxylin-eosin (H&E) staining revealed that PSE administration (500 mg/kg BW) improved follicular development and ovulation compared to the negative control group, as evidenced by a reduction in cystic follicle count and an increase in corpus luteum formation. In conclusion, PSE exhibits potential therapeutic effects in ameliorating PCOS-like symptoms by modulating reproductive hormones and promoting ovarian follicular maturation.
HIGHLIGHTS
- PCOS was successfully induced in Sprague Dawley rats through 42-day DHEA administration, resulting in characteristic conditions of the syndrome including hyperandrogenism and polycystic ovaries.
- Treatment with 500 mg/kg BW PSE significantly restored hormonal profiles by reducing LH, testosterone, and AGEs levels, while increasing FSH levels.
- Histopathological examination confirmed that the improved hormonal profiles directly correlated with ovarian structural restoration, as evidenced by enhanced follicular maturation.
GRAPHICAL ABSTRACT
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