The Steroids produced by Cladosporium anthropophilum, an endophytic fungus Isolated from Avicennia marina (Forssk.) Vierh and Their Antibacterial Activity
DOI:
https://doi.org/10.48048/tis.2024.8498Keywords:
Steroid, Endophytic fungi, Cladosporium anthropophilum, Avicennia marina, Antibacterial activityAbstract
This work was conducted to isolate the steroid compounds from an endophytic fungus Cladosporium anthropophilum and determine their effects against 4 strains of bacteria. The fungi were afforded from a mangrove plant Avicennia marina (Forssk.) Vierh of Acanthaceae family, growing in West Java, Indonesia. The fermentation and isolation of C. anthropophilum yielded 3 ergostane-type steroids including a highly oxidized one, identified as penicisteroid A (1), ergosterol (2), and ergosterol-5,8-peroxide (3), together with 2 stigmastanes, characterized as stigmasterol (4), and stigmasterol-5,8-peroxide (5). The elucidation structure of all isolated steroids was performed by extensive spectroscopic measurements (MS, IR, 1D, and 2D NMR) and supported by a comparison of previously reported spectral data. Subsequently, the potential of the 5 steroids as well as methanol, n-hexane, and ethyl acetate extracts were evaluated against 2 Gram-positive bacteria, Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 and 2 Gram-negative bacteria, Vibrio harveyi ATCC 5339 and Escherichia coli ATCC 25922. The results showed that none of the isolated steroids were active, while the 3 extracts exhibited significant inhibition against all the tested bacteria’s growth and enabled us to propose the synergistic effects between active compounds in these extracts and their antibacterial activity.
HIGHLIGHTS
- An isolated and identified endophytic fungus, Cladosporium anthropophilum, from a mangrove plant Avicennia marina (Forssk.) Vierh yielded five steroids.
- A chromatography technique and spectroscopic method were utilized for separation processes and structure determination, respectively.
- An antibacterial assay against two Gram-positive bacteria ( aureus and E. faecalis) and two Gram-negative bacteria (V. harveyi and E. coli) implied the synergistic effects between five isolated steroids and their parent extracts.
- A further investigation is highly recommended to explore the possibility of these five steroids as a potential source of active compounds.
GRAPHICAL ABSTRACT
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