Comparative Studies of Two Indonesian Medicinal Plants, Bidara Upas (Merremia mammosa Lour. Hall.f) and Adas (Foeniculum vulgare Miller): Antioxidant, Antidiabetic, and Antimicrobial Activities
DOI:
https://doi.org/10.48048/tis.2024.7868Keywords:
Antimicrobial, Antioxidant, α-amylase inhibitory, Merremia mammosa, Foeniculum vulgareAbstract
Bidara upas (Merremia mammosa) and adas (Foeniculum vulgare Miller) are 2 medicinal plants that grow abundantly in Indonesia. This study investigated the chemical composition and biological activity of M. mammosa and F. vulgare. Phytochemical screening was conducted on both plants. However, only the essential oil of F. vulgare was analyzed for GC spectra. A total of 23 compounds were identified from the essential oil of F. vulgare, with anethole being the main constituent (79.02 %). The extract of M. mammosa had a higher total phenolic content (TPC) and total flavonoid content (TFC) of 50.241 ± 0.012 mg gallic acid equivalent (GAE) per gram extract and 53.968 ± 0.742 mg quercetin equivalent (QRE) per gram extract, respectively. In the DPPH antioxidant test, the IC50 value indicated that M. mammosa extract had better activity than F. vulgare, with an IC50 of 89.139 ± 0.189 μgmL−1. This is supported by the TAC (Total Antoxidant Capacity) and Reducing Power Hexacyanoferrate (III) values, which also show a better antioxidant potential in M. Mammosa than in F. vulgare. In addition, M. mammosa has been shown to have an IC50 value of 2.215 ± 0.015 mgmL−1 against α-amylase inhibition, which is slightly different from F. vulgare with an IC50 value of 2.467 ± 0.025 mgmL−1. Overall, the antimicrobial activity of F. vulgare and M. mammosa was found to be resistant against S. mutans bacteria. The ethanol extract of F. vulgare was more potent against gram-positive bacteria and the essential oil was only effective against fungi. On the other hand, the ethanol extract of M. mammosa and the combination of their ethanol extracts showed no significant activity against bacteria and fungi. The combination of water extracts is most effective against 2 microbes, namely S. epidermis and E. faecalis with MIC 7.81 and 3.91 mgmL−1.
HIGHLIGHTS
- The chemical composition of vulgare essential oil was characterised by GC-MS following experimental hydrodistillation.
- The ethanol extract of mammosa exhibited superior activity towards antioxidant and alpha-amylase inhibition compared to the ethanol extract of F. vulgare.
- The combination of aqueous extracts of mammosa and F. vulgare only showed activity against S. epidermis and E. faecalis.
- Our findings reveal that mammosa exhibited some promising antioxidant properties, while F. vulgare demonstrated some potential for antimicrobial activity.
GRAPHICAL ABSTRACT
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A Yanuar, A Mun’im, A Bertha, A Lagho, RR Syahdi, M Rahmat and H Suhartanto. Medicinal plants database and three dimensional structure of the chemical compounds from medicinal plants in Indonesia. Int. J. Comput. Sci. 2011; 5, 180-3.
M Silalahi, Nisyawati, EB Walujo, J Supriatna and W Mangunwardoyo. The local knowledge of medicinal plants trader and diversity of medicinal plants in the Kabanjahe traditional market, North Sumatra, Indonesia. J. Ethnopharmacol. 2015; 175, 432-43.
IW Kusuma, Murdiyanto, ET Arung, Syafrizal and Y Kim. Antimicrobial and antioxidant properties of medicinal plants used by the Bentian tribe from Indonesia. Food Sci. Hum. Wellness 2014; 3, 191-6.
RD Jong and CV Achterberg. Global disjunctions and flying insects. In: W Renema (Ed.). Biogeography, time and place: Distributions, barriers and islands. Springer, Dordrecht, Netherlands, 2007, p. 5-44.
K von Rintelen, E Arida and C Häuser. A review of biodiversity-related issues and challenges in megadiverse Indonesia and other Southeast Asian countries. Res. Ideas Outcome. 2017; 3, e20860.
HM Sangat and I Larasati. Some ethnophytomedical aspects and conservation strategy of several medicinal plants in Java, Indonesia. Biodiversitas 2002; 3, 231-5.
M Mangestuti, S Subehan, A Widyawaruyanti, SFH Zaidi, S Awale and S Kadota. Traditional medicine of Madura Island in Indonesia. J. Tradit. Med. 2007; 24, 90-103.
HA Hariana. 262 tumbuhan obat dan khasiatnya (in Indonesian). Penebar Swadaya, Jakarta, Indonesia, 2013.
P Utami and DE Puspaningtyas. The miracle of herbs. AgroMedia Pustaka, Jakarta, Indonesia, 2013.
N Purwitasari and M Agil. Metabolite profiling of extract and fractions of bidara upas (Merremia mammosa (Lour.) Hallier F.) tuber using UPLC-QToF-MS/MS. Biomed. Pharmacol. J. 2022; 15, 2025-41.
S Dalimartha. Atlas tumbuhan obat Indonesia (in Indonesian). Vol I. Trubus Agriwidya, Jakarta, Indonesia, 1999.
Y Li, AS Fabiano-Tixie and F Chemat. Essential oils as reagents in green chemistry. Springer Avignon, France, 2014.
H Sastrohamidjojo. Kimia minyak atsiri (in Indonesian). UGM Press, Yogyakarta, Indonesia, 2021.
MA Rather, BA Dar, SN Sofi, BA Bhat and MA Qurishi. Foeniculum vulgare: A comprehensive review of its traditional use, phytochemistry, pharmacology, and safety. Arabian J. Chem. 2016; 9, S1574-S1583.
SB Badgujar, VV Patel and AH Bandivdekar. Foeniculum vulgare Mill: A review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology. Biomed. Res. Int. 2014; 2014, 842674.
R Rahimi and MRS Ardekani. Medicinal properties of Foeniculum vulgare Mill. in traditional Iranian medicine and modern phytotherapy. Chin. J. Integr. Med. 2013; 19, 73-9.
JR Shaikh and M Patil. Qualitative tests for preliminary phytochemical screening: An overview. Int. J. Chem. Stud. 2020; 8, 603-8.
H Noreen, N Semmar, M Farman and JSO McCullagh. Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pac. J. Trop. Med. 2017; 10, 792-801.
M Ondua, EM Njoya, MA Abdalla and LJ McGaw. Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation. J. Ethnopharmacol. 2019; 234, 27-35.
K Loucif, H Benabdallah, F Benchikh, S Mehlous, CB Souici and S Amira. Total phenolic contents, DPPH radical scavenging and β-carotene bleaching activities of aqueous extract from Ammoides atlantica. J. Drug. Deliv. Ther. 2020; 10, 196-8.
KM Schaich, X Tian and J Xie. Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays. J. Funct. Foods 2015; 14, 111-25.
G Morales and A Paredes. Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. BMC Compl. Alternative Med. 2014; 14, 259.
X Yang, F Yan, S Huang and C Fu. Antioxidant activities of fractions from longan pericarps. Food Sci. Tech. 2014; 34, 341-5.
A Yumita, E Hanani, A Agustina, F Damayanti, KN Priani and SN Fadila. Total phenolic content and antioxidant activities of leaves and bark extract of Adenanthera pavonina L. Nat. Prod. Sci. 2023; 29, 24-30.
U Taukoorah and MF Mahomoodally. Crude Aloe vera gel shows antioxidant propensities and inhibits pancreatic lipase and glucose movement in vitro. Adv. Pharmacol. Sci. 2016; 2016, 3720850.
R Schwalbe, L Steele-Moore and AC Goodwin. Antimicrobial susceptibility testing protocols. CRC Press, New York, 2007.
CG Giske, J Turnidge, R Cantón and G Kahlmeter. Update from the European committee on antimicrobial susceptibility testing (EUCAST). J. Clin. Microbiol. 2022; 60, e00276-21.
MA Pfaller, M Castanheira, DJ Diekema, SA Messer, GJ Moet and RN Jones. Comparison of European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Etest methods with the CLSI broth microdilution method for echinocandin susceptibility testing of Candida species. J. Clin. Microbiol. 2010; 48, 1592-9.
PR Hsueh, WC Ko, JJ Wu, JJ Lu, FD Wang, HY Wu, TL Wug and LJ Teng. Consensus statement on the adherence to Clinical and Laboratory Standards Institute (CLSI) antimicrobial susceptibility testing guidelines (CLSI-2010 and CLSI-2010-update) for enterobacteriaceaein clinical microbiology laboratories in Taiwan. J. Microbiol. Immunol. Infect. 2010; 43, 452-5.
R Leclercq, R Cantón, DFJ Brown, CG Giske, P Heisig, AP Macgowan, JW Mouton, P Nordmann, AC Rodloff, GM Rossolini, CJ Soussy, M Steinbakk, TG Winstanley and G Kahlmeter. EUCAST expert rules in antimicrobial susceptibility testing. Clin. Microbiol. Infect. 2013; 19, 141-60.
EL Berkow, SR Lockhart and L Ostrosky-Zeichner. Antifungal susceptibility testing: Current approaches. Clin. Microbiol. Rev. 2020; 33, e00069-19.
M Balouiri, M Sadiki and SK Ibnsouda. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharmaceut. Anal. 2016; 6, 71-9.
JH Jorgensen, KC Carroll, G Funke, MA Pfaller, ML Landry, SS Richter and DW Warnock. Manual of clinical microbiology. Vol I. 11th ed. ASM Press, Washington DC, 2015.
F Sharopov, A Valiev, P Satyal, I Gulmurodov, S Yusufi, WN Setzer and M Wink. Cytotoxicity of the essential oil of fennel (Foeniculum vulgare) from Tajikistan. Foods 2017; 6, 73.
İ Gulcin and SH Alwasel. DPPH radical scavenging assay. Processes 2023; 11, 2248.
I Aouam, YE Atki, M Taleb, A Taroq, FE Kamari, B Lyoussi and A Abdellaoui. Antioxidant capacities and total phenolic contents of Thymus riatarum. In: Proceedings of the International Conference on Materials and Environmental Science, Oujda, Morocco. 2019.
RK Ameta, RR Koshti, A Vyas, C Rane, NK Sharma and M Singh. [Fe(CN)6]4−/[Fe(CN)6]3− based metal organic ionic frameworks and impact of Fe2+/Fe3+ on material-medicinal-properties. J. Mol. Liq. 2018; 268, 677-84.
MR Bhandari, N Jong-Anurakkun, G Hong and J Kawabata. α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chem. 2008; 106, 247-52.
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