Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes

Authors

  • Vinda Zakiyatuz Zulfa Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Sepuluh Nopember Technology Institute, Surabaya 60111, Indonesia
  • Ulya Farahdina Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Sepuluh Nopember Technology Institute, Surabaya 60111, Indonesia
  • Ihwanul Aziz Research Center for Accelerator Technology, Research Organization of Nuclear Energy, National Research and Innovation Agency, Yogyakarta 55281, Indonesia
  • Nasori Nasori Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Sepuluh Nopember Technology Institute, Surabaya 60111, Indonesia
  • Endarko Endarko Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Sepuluh Nopember Technology Institute, Surabaya 60111, Indonesia
  • Muslikha Nourma Rhomadhoni Occupational and Safety Department, Universitas Nahdlatu Ulama Surabaya, Surabaya 60237, Indonesia
  • Agus Rubiyanto Laboratory Medical Physics and Biophysics, Department of Physics, Faculty of Sciences and Data Analytic, Sepuluh Nopember Technology Institute, Surabaya 60111, Indonesia

DOI:

https://doi.org/10.48048/tis.2023.7041

Keywords:

Albumin, Biomarker, Blood cancer, Cyclic voltammetry, Electrode, Electrochemical impedance spectroscopy, Silver

Abstract

Ag and AgNO film were fabricated through sputter deposition on indium tin oxide (ITO) conductive glass. Electrochemical detection using cyclic voltammetry (CV) was carried out for low albumin levels in blood as a parameter of cancer patients. This study aims to determinate low albumin level as a biomarker cancer. Albumin adsorption on the Agfilm/ITO and AgNOfilm/ITO was also investigated to determine the level of sensitivity of both electrodes. Analysis of the CV measurements indicated that the Agfilm/ITO electrode was more sensitive compared to the AgNOfilm/ITO electrode, with a sensitivity value of 4.564 µA M-1cm-2 for the Agfilm/ITO electrode and 2.123 µA M-1cm-2 for the AgNOfilm/ITO electrode. The testing of albumin levels in blood within a range of low concentration levels between 10-1 and 10-8 g/dL indicated a detection limit of 10-9 g/mL. The selectivity of Ag electrodes was found to be very good for other interfering molecules such as urine, glucose, and lysozyme. The results of the modeling of electric field and magnetic field distribution showed that the Agfilm/ITO electrode possessed larger values than the AgNOfilm/ITO electrode. From this analysis, it can be concluded that the electrode modified with micro-sized Ag achieved more effective results than the electrode modified with AgNO. This sensor can determine low albumin level as a blood cancer biomarker. This sensor holds great promise for use in real samples in the future.

HIGHLIGHTS

  • Early detection with albumin biomarkers before metastasis cancer
  • The testing of albumin levels with Cyclic Voltammetry and Electric Impedance Spectroscopy in the cancer within a wide range of low concentration levels (10-1 - 10-8) indicated a detection limit of 10-9 g/mL.
  • The total mass of adsorbed BSA in electrode surface is more higher (281 mgm-2) than previous study.
  • The Agfilm/ITO electrode exhibited high sensitivity and long-term stability for future applications.

GRAPHICAL ABSTRACT

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Published

2023-09-02

How to Cite

Zulfa, V. Z. ., Farahdina, U. ., Aziz, I. ., Nasori, N. ., Endarko, E. ., Rhomadhoni, M. N. ., & Rubiyanto, A. . (2023). Low Albumin Determination as a Biomarker Cancer with Cyclic Voltammetry in Agfilm/ITO and AgNOfilm/ITO Electrodes. Trends in Sciences, 20(12), 7041. https://doi.org/10.48048/tis.2023.7041

Issue

Vol. 20 No. 12 (2023): Trends in Sciences, Volume 20, Number 12, December 2023

Section

Research Articles

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