Physicochemical Properties of Rice Bran Hydrolysate Prepared in a Pilot Scale Process and Its Application in Milk Tablets
DOI:
https://doi.org/10.48048/tis.2022.2316Keywords:
Milk tablets, Pilot scale, Rice bran hydrolysate, Antioxidant activity, Consumer testAbstract
The objectives of this study were to produce rice bran hydrolysate (RBH) in a pilot-scale process and determine its physicochemical properties including chemical compositions, α-amino contents, molecular weight (MW) distribution and antioxidant activities. The results found that RBH contained a crude protein of 16.14 %, α-amino contents of 51.56 mg Leucine Eq./g RBH, ABTS radical scavenging activity of 20.17 mg Trolox Eq./g RBH, and FRAP value of 5.77 mg Trolox Eq./g RBH. The major peak of MW peptides of RBH was 753 - 6,088 Da (50.81 %). RBH in various levels (0 - 2.0 %) was supplemented in milk tablets. Physicochemical properties, and sensory evaluation (9-point hedonic scale) of all milk tablets were then determined. The result indicated that the milk tablets with 2.0 % RBH (MT_2%RBH) contained higher antioxidant activities than the others, however, the overall acceptability score of all milk tablets was not significantly different (p ≥ 0.05). MT_2 %RBH was selected for further development by adding 2.0 % cocoa (MT_2%RBHC) and 2.0 % vanilla powder (MT_2%RBHV). The MT_2%RBHC containing higher antioxidant activities and sensory attributes score than the others was further selected for microbiological qualities, consumer and purchase tests (n = 300). It was found that MT_2%RBHC was accepted by 300 people, with an overall acceptability score of 7.16 (Like moderately), and its microbiological qualities, including total bacteria, total yeast and mold and Escherichia coli did not exceed the standard limits. These results suggested that RBH produced in the pilot-scale process has a potential as nutritive and antioxidative supplementation in milk tablets.
HIGHLIGHTS
- Rice bran hydrolysate (RBH) has a potential for production in a pilot-scale process
- The major peak of molecular weight (MW) peptides of RBH was 753 - 6,088 Da (50.81 %)
- RBH has a potential as antioxidative supplementation in milk tablets
- Cocoa milk tablets supplemented with RBH were accepted by 300 panelists
- Microbiological qualities of milk tablet did not exceed the standard limits
GRAPHICAL ABSTRACT 
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References
United States Department of Agriculture (USDA), Available at:
https://fdc.nal.usda.gov/fdc-app.html#/food-details/1097512/nutrients, accessed March 2022.
A Kamlangdee, S Saiut, S Upasen and P Wattanachai. Milk tablet processing techniques for novel milk product. In: Proceedings of the Academicsera 17th International Conference, Istanbul, Turkey. 2018, p. 25-9.
The Nation Thailand, Available at: https://www.nationthailand.com/in-focus/30378894, accessed March 2022.
DB Kapadiya, DB Prajapati, AK Jain, BM Mehta, VB Darji and KD Aparnathi. Comparison of Surti goat milk with cow and buffalo milk for gross composition, nitrogen distribution, and selected minerals content. Vet. World 2016; 9, 710-6.
Open Food Facts, Available at: https://world.openfoodfacts.org/category/milk-tablet, accessed March 2022.
United States Department of Agriculture (USDA), Available at: http://www.aseanthai.net/english/mobile_detail.php?cid=14&nid=4030, accessed December 2021.
SASC Faria, PZ Bassinello and MVC Penteado. Nutritional composition of rice bran submitted to different stabilization procedures. Braz. J. Pharm. Sci. 2012; 48, 651-7.
BS Luh. Rice oil. In: SB Luh (Ed.). Rice Production. 2nd ed. Springer Publishing, New York, 1991, p. 714-30.
M Wang, NS Hettiarachchy, M Qi, W Burks and T Siebenmorgen. Preparation and functional properties of rice bran protein isolate. J. Agr. Food Chem. 1999; 47, 411-6.
X Wang, H Chen, X Fu, S Li and J Wei. A novel antioxidant and ACE inhibitory peptide from rice bran protein: Biochemical characterization and molecular docking study. LWT Food Sci. Tech. 2017; 75, 93-9.
C Uraipong and J Zhao. In vitro digestion of rice bran proteins produces peptides with potent inhibitory effects on alpha-glucosidase and angiotensin I converting enzyme. J. Sci. Food Agr. 2018; 98, 758-66.
JJ Fan, X Luo and Z Dong. Extraction isolation and purification of rice bran peptides. Food Sci. Tech. 2008; 33, 169-72.
X Zhang, Q Chen and G Pang. The preparation of the oligopeptides and the investigations of the antioxidative activity of oligopeptides from the corn gluten meal. J. Food Sci. 2005; 26, 33-5.
P Thamnarathip, K Jangchud, S Nitisinprasert and B Vardhanabhuti. Identification of peptide molecular weight from rice bran protein hydrolysate with high antioxidant activity. J. Cereal Sci. 2016; 69, 329-35.
C Khongla, S Musika, P Sangsawad, L Srithamma, P Kiatbenjakul and V Tanamool. Antioxidant activity of Jasmine rice bran hydrolysate and its application in broken rice beverages. Suranaree J. Sci. Tech. 2022; 29, 1-10.
K Chen, J Zhao, X Shi, Q Abdul and Z Jiang. Characterization and antioxidant activity of products derived from xylose - bovine casein hydrolysate Maillard reaction: Impact of reaction time. Foods 2019; 8, 242.
AOAC. Official methods of analysis. 17th ed. Association of Official Chemists, Gaithersberg, Maryland, United States, 2000.
J Adler-Nissen. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J. Agr. Food Chem. 1979; 27, 1256-62.
C Wiriyaphan, B Chitsomboon and J Yongsawadigul. Antioxidant activity of protein hydrolysates derived from threadfin bream surimi byproducts. Food Chem. 2012; 132, 104-11.
IFF Benzie and JJ Strain. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power the FRAP assay. Anal. Biochem. 1996; 239, 70-6.
C Khongla, S Musika and P Sangsawad. Changes in antioxidant activity of fermented fish sauce (Nam Pla-ra) from Osteochilus Hasseltii during fermentation. Suranaree J. Sci. Tech. 2020; 27, 1-8.
L Maturin and JT Peeler. BAM chapter 3: Aerobic plate count, Available at: https://www.fda.gov/ Food/FoodScienceResearch/LaboratoryMethods/ucm063346.htm, accessed August 2021.
V Tournas, EM Stack, BP Mislivec, AH Koch and R Bandler. BAM: Yeasts, molds and mycotoxins, Available at: https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm071435.htm, accessed August 2021.
P Feng, SD Weagant, MA Grant, W Burkhardt, M Shellfish and B Water. BAM: Enumeration of Escherichia coli and the Coliform Bacteria, Available at: http://www.fda.gov/Food/FoodScience Research/LaboratoryMethods/ucm064948.htm, accessed August 2021.
AP Adebiyi, AO Adebiyi, J Yamashita, T Ogawa and K Muramoto. Purification and characterization of antioxidative peptides derived from rice bran protein hydrolysates. Eur. Food Res. Tech. 2009; 228, 553-63.
P Goupy, C Dufour, M Loonis and O Dangles. Quantitative kinetic analysis of hydrogen transfer reactions from dietary polyphenols to the DPPH radical. J. Agr. Food Chem. 2003; 51, 615-22.
S Nanasombat, K Yansodthee and I Jongjaited. Evaluation of antidiabetic, antioxidant and other phytochemical properties of Thai fruits, vegetables and some local food plants. Walailak J. Sci. Tech. 2019; 16, 851-66.
P Yongpradoem and N Weerapreeyakul. Evaluation of antioxidant activity and inhibition of tyrosinase activity of Raphanus sativusvar. caudatus Alef extract. Walailak J. Sci. Tech. 2020; 17, 838-50.
J Adler-Nissen. Enzymic hydrolysis of food proteins. Elsevier Applied Science Publishers, New York, 1986, p. 110-85.
P Thamnarathip, K Jangchud, S Nitisinprasert and B Vardhanabhuti. Functional properties of protein hydrolysates from Riceberry rice bran. Int. J. Food Sci. 2016; 51, 1110-9.
X Zhang, YL Xiong, J Chen and L Zhou. Synergy of licorice extract and pea protein hydrolysate for oxidative stability of soybean oil-in-water emulsions. J. Agr. Food Chem. 2014; 62, 8204-13.
MBK Foh, I Amadou, BM Foh, MT Kamara and WS Xia. Functionality and antioxidant properties of Tilapia (Oreochromis niloticus) as influenced by the degree of hydrolysis. Int. J. Mol. Sci. 2010; 11, 1851-69.
S Phongthai, S D'Amico, R Schoenlechner, W Homthawornchoo and S Rawdkuen. Fractionation and antioxidant properties of rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion. Food Chem. 2018; 240, 156-64.
E Nguyen, O Jones, YHB Kim, F Martin‐Gonzalez and AM Liceaga. Impact of microwave‐assisted enzymatic hydrolysis on functional and antioxidant properties of rainbow trout Oncorhynchus mykiss by‐products. Fish Sci. 2017; 83, 317-31.
JM Kim, AM Liceaga and KY Yoon. Purification and identification of an antioxidant peptide from perilla seed (Perilla frutescens) meal protein hydrolysate. Food Sci. Nutr. 2019; 7, 1645-55.
L Pizzoferrato, P Manzi, V Vivanti, I Nicoletti, C Corradini and E Cogliandro. Maillard reaction in milk-based foods: Nutritional consequences. J. Food Protect. 1998; 61, 235-9.
N Phisut and B Jiraporn. Characteristics and antioxidant activity of Maillard reaction products derived from chitosan-sugar solution. Food Res. Int. 2013; 20, 1077-85.
S Stojanovska, N Gruevska, J Tomovska, J Tasevska, A Krstanovski and M Menkovska. Maillard reaction and lactose structural changes during milk processing. J. Chem. Res. 2017; 2, 139-45.
Notification of the Ministry of Public Health (No.351), Available at: https://www.food.fda.moph.go.th/law/data/ announ_ moph /V.English/P351_E.pdf, accessed August 2021.
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