Role of RyR2 and SERCA2a in the Cardioprotective Effects of Vincanine and Pyrazoline Alkaloids

Authors

  • Inoyat Zulfiqorovich Zhumaev Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Sadriddin Nurillo Ugli Boboev Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Pulat Bekmuratovich Usmanov Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Shavkat Yusubovich Rustamov Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Abdisalim Abdikarimovich Zaripov Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Shakhnoza Bakhtiyorovna Qurbonova Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Yulduzkhon Takhirjanovna Mirzaeva Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Eldor Bakhtiyor Ugli Ibragimov Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Adilbay Tlepovich Esimbetov Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
  • Shahobiddin Mukhammadovich Adizov Institute of Chemistry of Plant Substances, Academy Sciences of Uzbekistan, Tashkent, Uzbekistan

DOI:

https://doi.org/10.48048/tis.2025.8626

Keywords:

Indole alkaloid, Papillary muscle, Inotropic activity, Sarcoplasmic reticulum, Ion-channels

Abstract

In the conducted studies, the effect of vincanine and pyrazoline indole alkaloids on cardiac muscle cell sarcoplasmic reticulum (SR) Ca2+-transport systems (RyR2 and SERCA2a) was investigated under normal and hypoxic conditions. The effect of vincanine and pyrazoline on ryanodine receptor (RyR2) was evaluated in the presence of ruthenium red and it was found that the role of RyR2 in the positive inotropic effect of these alkaloids is small. Also, in the presence of cyclopiazonic acid (IC50 = 5.6 µM), an inhibitor of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), the positive inotropic effect of vincanine and pyrazoline alkaloids was reduced. In general, the positive inotropic effects and cardioprotective properties of vincanine and pyrazoline alkaloids are mediated by modulation of SERCA2a function.

HIGHLIGHTS

  • Vincanine and pyrazoline indole alkaloids have a positive inotropic effect on the force of papillary muscle contraction of the rat heart.
  • Vincanine and pyrazoline indole alkaloids have a cardioprotective effect and effectively eliminate the disturbances in the papillary muscle contractile activity of the rat heart caused by hypoxia.
  • Vincanine and pyrazoline alkaloids effectively reverse hypoxia-induced SERCA2a dysfunction and normalize changes in rat cardiac papillary muscle contractile activity.

GRAPHICAL ABSTRACT

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Published

2024-11-15

Issue

Vol. 22 No. 1 (2025): Trends in Sciences, Volume 22, Number 1, January 2025

Section

Research Articles

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