Optimization, Purification and Characterization of Lipase from Streptomyces sp. A3301, with Application of Crude Lipase for Cooking Oily Wastewater Treatment
DOI:
https://doi.org/10.48048/tis.2024.8504Keywords:
Lipase, Streptomyces sp. A3301, Optimization, Lipase production, Purification and characterization of lipase, Oily wastewater treatment, ImmobilizationAbstract
Streptomyces sp. A3301, which produces lipase isolated by Panyachanakul et al. [1]. This optimization was done for the subsequent purification and characterization of the biological lipase produced by the isolate. The results showed that the strain produced lipase with a maximum activity of 321 U/mL using the optimal medium and conditions (1.5 % (w/v) xylose and 2 % (w/v) yeast extract, pH 7.0 at 30 °C, 150 rpm for 3 days). The specific activity of the purified lipase was 27,000 U/mg, which was 544 times the pre-purification level, based on hydrophobic chromatography. After ion-exchange chromatography, the specific activity was 5,600 U/mg, which was 113 times the pre-purification level, with a single-peak purification profile. The purified lipase had a single band based on SDS-PAGE analysis and the molecular mass was 45 kDa. The optimum temperature and thermo-stability of A3301 lipase were 60 and 30 - 55 °C, respectively. The optimum pH of the purified enzyme was pH 9.0, and the enzyme was stable in the pH range of 8.0 - 9.0. The purified lipase was stable in acetone, chloroform and toluene, with a high relative activity of 63 - 76 %. The immobilized strain was then applied to oily-wastewater treatment. The strain can remove oil and grease in synthetic wastewater containing oil at 1 - 3 % (v/v), with removal rates of 100, 99.82 and 99.68 %, respectively, after incubation for 6 days. It was then applied to oily-wastewater treatment from a restaurant, achieving the highest degradation rates of 98.53 % after treatment for 6 days. In addition, it also affected the Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) values decreasing.
HIGHLIGHTS
- Optimization of lipase production by Streptomyces A3301.
- Purification and characterization of the lipase produced by the Streptomyces A3301.
- Application of the immobilized Streptomyces A3301 strain in the treatment of oily cooking wastewater.
- The immobilized Streptomyces A3301 strain has the capability to remove oil and grease in both synthetic mediums and restaurant wastewater.
GRAPHICAL ABSTRACT
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T Panyachanakul, T Lomthong, W Lorliam, J Prajanbarn, S Tokuyama, V Kitpreechavanich and S Krajangsang. New insight into thermo-solvent tolerant lipase produced by Streptomyces sp. A3301 for re-polymerization of poly (dl-lactic acid). Polymer 2020; 204, 122812.
MB Moghaddam, R Hemmati, A Homaei, F Vianello and B Shareghi. Novel cold-adapted lipase from Psychrobacter sp. C18 immobilized on reduced graphene oxide-cellulose nanomatrix with high activity and stability. J. Mol. Liq. 2024; 397, 124210.
D Bharathi and G Rajalakshmi. Microbial lipases: An overview of screening, production and purification. Biocatal. Agric. Biotechnol. 2019; 22, 101368.
R Gupta, N Gupta and P Rathi. Bacterial lipases: An overview of production, purification and biochemical properties. Appl. Microbiol. Biotechnol. 2004; 64, 763-81.
HL Lau, FWF Wong, RNZRA Rahman, MS Mohamed, AB Ariff and SL Hii. Optimization of fermentation medium components by response surface methodology (RSM) and artificial neural network hybrid with genetic algorithm (ANN-GA) for lipase production by Burkholderia cenocepacia ST8 using used automotive engine oil as substrate. Biocatal. Agric. Biotechnol. 2023; 50, 102696.
P Paitaid, J Buatong, S Phongpaichit and A H-kittikun. Purification and characterization of an extracellular lipase produced by Aspergillus oryzae ST11 as a potential catalyst for an organic synthesis. Trends Sci. 2021; 18, 45.
S Javed, F Azeem, S Hussain, I Rasul, MH Siddique, M Riaz, M Afzal, A Kouser and H Nadeem. Bacterial lipases: A review on purification and characterization. Prog. Biophys. Mol. Biol. 2018; 132, 23-34.
VD Nimkande and A Bafana. A review on the utility of microbial lipases in wastewater treatment. J. Water Process Eng. 2022; 46, 102591.
R Boran, A Ugur, N Sarac and O Ceylan. Characterisation of Streptomyces violeascens OC125-8 lipase for oily wastewater treatment. 3 Biotech 2019; 9, 5.
JE Lesny, AP Mohan and JA Vijaya. Bioremediation of petroleum-polluted soil using biosurfactant producting bacteria, Pseudomonas sp. J. Sci. Res. 2022; 66, 224-31.
LM Blandón, MA Marín, M Quintero, LM Jutinico-Shubach, M Montoya-Giraldo, M Santos-Acevedo and J Gómez-León. Diversity of cultivable bacteria from deep-sea sediments of the Colombian Caribbean and their potential in bioremediation. Antonie Van Leeuwenhoek 2022; 115, 421-31.
P Welz, G Swanepoel, S Weels and M le Roes-Hill. Wastewater from the edible oil industry as a potential source of lipase-and surfactant-producing actinobacteria. Microorganisms 2021; 9, 1987.
K Selvam and B Vishnupriya. Partial purification of lipase from Streptomyces variabilis NGP3 and its application in bioremediation of waste water. Int. J. Pharm. Sci. Res. 2013; 4, 4281-9.
SS Cho, DJ Park, JR Simkhada, JH Hong, JK Sohng, OH Lee and JC Yoo. A neutral lipase applicable in biodiesel production from a newly isolated Streptomyces sp. CS326. Bioprocess Biosyst. Eng. 2012; 35, 227-34.
E Blanchet, E Desmond, B Erable, A Bridier, T Bauchez and A Bargel. Comparison of synthetic medium and wastewater used as dilution medium to design scalable microbial anodes: Application to food waste treatment. Bioresour. Tech. 2015; 185, 106-15.
R Sharma, Y Chisti and UC Banerjee. Production, purification, characterization, and applications of lipases. Biotechnol. Adv. 2001; 19, 627-62.
S Ananthi, G Immanuel and A Palavesam. Optimization of lipase production by Bacillus cereus strain MSU AS through submerged fermentation. Plant Sci. Feed 2013; 3, 31-9.
E Nahas. Control of lipase production by Rhizopus oligosporus under various growth conditions. J. Gen. Microbiol. 1988; 134, 227-33.
V Sutar and J Kurhekar. Isolation and characterization of lipase producing bacteria from restaurant waste water. World J. Pharm. Res. 2017; 6, 685-93.
D Bharathi, G Rajalakshmi and S Komathi. Optimization and production of lipase enzyme from bacterial strains isolated from petrol spilled soil. J. King Saud Univ. Sci. 2019; 31, 898-901.
H Mazhar, N Abbas and A Sohall. Optimized production of lipase from Bacillus subtilis PCSIRNL-39. Afr. J. Biotechnol. 2017; 16, 1106-15.
P Mander, SS Cho, JR Simkhada, YH Choi, DJ Park, JW Ha and JC Yoo. Organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production. Biotechnol. Bioprocess Eng. 2012; 17, 67-75.
NS Rios, BB Pinheiro, MP Pinheiro, RM Bezerra, JCS dos Santos and LRB Goncalves. Biotechnological potential of lipases from Pseudomonas: Sources, properties and applications. Process Biochem. 2018; 75, 99-120.
AF de Almeida, SM Taulk-Tornisielo and EC Carmona. Influence of carbon and nitrogen sources on lipase production by a newly isolated Candida viswanathii strain. Ann. Microbiol. 2013; 63, 1225-34.
LP Christopher, VP Zambare, A Zambare, H Kumar and L Malek. A thermo-alkaline lipase from a new thermophile Geobacillus thermodenitrificans AV-5 with potential application in biodiesel production. J. Chem. Tech. Biotechnol. 2015; 90, 2007-16.
S Li and HY Yu. Characterization of a novel extracellular lipase from a halophilic isolate, Chromohalobacter sp. LY7-8. Afr. J. Microbiol. Res. 2012; 6, 3516-22.
S Qin, Y Zhao, B Wu and B He. A calcium-ion-stabilized lipase from Pseudomonas stutzeri ZS04 and its application in resolution of chiral aryl alcohols. Appl. Biochem. Biotechnol. 2016; 180, 1456-66.
A Abubakar, PO Abioye, SA Aransiola and MN Raju. Crude oil biodegradation potential of lipase produced by Bacillus subtilis and Pseudomonas aeruginosa isolated from hydrocarbon contaminated soil. Environ. Chem. Ecotoxicol. 2024; 6, 26-32.
H Patel, S Ray, A Patel, K Patel and U Trivedi. Enhanced lipase production from organic solvent tolerant Pseudomonas aeruginosa UKHL1 and its application in oily waste-water treatment. Biocatal. Agric. Biotechnol. 2020; 28, 101731.
VDH Nguyen, TNP Huynh, TTT Nguyen, HH Ho, LTP Trinh and AQ Nguyen. Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil. Protein Expression Purif. 2024; 215, 106419.
S Bunmadee, J Teeka, T Lomthong, D Kaewpa, P Areesirisuk and A Areesirisuk. Isolation and identification of a newly isolated lipase-producing bacteria (Acinetobacter baumannii RMUTT3S8-2) from oily wastewater treatment pond in a poultry processing factory and its optimum lipase production. Bioresour. Tech. Rep. 2022; 20, 101267.
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