Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175
DOI:
https://doi.org/10.48048/tis.2021.37Keywords:
Poly(butylene succinate) (PBS), Polybutylene succinate/poly(lactide) (PBS/PLA) blend, Laceyella sacchari LP175, Serine protease production, BiodegradationAbstract
The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 °C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 °C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste.
HIGHLIGHTS
- Upscaled serine protease production was achieved in a 10 L airlift fermenter by sacchari LP175 using low-cost agricultural products as substrate
- The crude enzyme degraded PBS and PBS/PLA powders (100 g/L) at up to 51.4 and 56.9 %, respectively in a 2.0 L stirrer fermenter under optimal conditions
- Degradation products of PBS and PBS/PLA by crude enzyme produced from sacchari LP175 were characterized
GRAPHICAL ABSTRACT
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K Thirunavukarasu, S Purushothaman, J Sridevi, M Aarthy, MK Gowthaman, T Nakajima-Kambe and NR Kamini. Degradation of poly(butylene succinate) and poly(butylene succinate-co-butylene adipate) by a lipase from yeast Cryptococcus sp. grown on agro-industrial residues. Int. Biodeter. Biodegr. 2016; 110, 99-107.
X Hu, T Su, P Li and Z Wang. Blending modification of PBS/PLA and its enzymatic degradation. Polymer Bull. 2018; 75, 533-46.
K Shi, Z Bai, T Su and Z Wang. Selective enzymatic degradation and porous morphology of poly (butylene succinate)/poly (lactic acid) blends. Int. J. Biol. Macromol. 2019; 126, 436-42.
S Su, R Kopitzky, S Tolga and S Kabasci. Polylactide (PLA) and its blends with poly (butylene succinate)(PBS): A brief review. Polymers 2019; 11, 1193.
N Jacquel, F Freyermouth, F Fenouillot, A Rousseau, JP Pascault, P Fuertes and R Saint-Loup. Synthesis and properties of poly(butylene succinate): Efficiency of different transesterification catalysts. J. Polymer Sci. A Polym. Chem. 2011; 49, 5301-12.
M Puchalski, G Szparaga, T Biela, A Gutowska, S Sztajnowski and I Krucinska. Molecular and supramolecular changes in polybutylene succinate (PBS) and polybutylene succinate adipate (PBSA) copolymer during degradation in various environmental conditions. Polymers 2018; 10, 251.
MS Singhvi, SS Zinjarde and DV Gokhale. Polylactic acid: Synthesis and biomedical applications. J. Appl. Microbiol. 2019; 127, 1612-26.
P Shaiju, BB Dorian, R Senthamaraikannan and RB Padamati. Biodegradation of poly(butylene succinate)(PBS)/stearate modified magnesium-aluminium layered double hydroxide composites under marine conditions prepared via melt compounding. Molecules 2020; 25, 5766.
K Shi, T Su and Z Wang. Comparison of poly (butylene succinate) biodegradation by Fusarium solani cutinase and Candida antarctica lipase. Polymer Degrad. Stabil. 2019; 164, 55-60.
W Penkhrue, C Khanongnuch, K Masaki, W Pathom-aree, W Punyodom and S Lumyong. Isolation and screening of biopolymer-degrading microorganisms from Northern Thailand. World J. Microbiol. Biotechnol. 2015; 31, 1431-42.
S Hanphakphoom, N Maneewong, S Sukkhum, S Tokuyama and V Kitpreechavanich. Characterization of poly (L-lactide)-degrading enzyme produced by thermophilic filamentous bacteria Laceyella sacchari LP175. J. Gen. Appl. Microbiol. 2014; 60, 13-22.
T Lomthong, M Guicherd, G Cioci, S Duquesne, A Marty, S Lumyong and V Kitpreechavanich. Poly (L-lactide)-degrading enzyme from Laceyella sacchari LP175: Cloning, sequencing, expression, characterization and its hydrolysis of poly (L-lactide) polymer. Chiang Mai J. Sci. 2019 46, 417-430.
T Lomthong, S Hanphakphoom, P Kongsaeree, N Srisuk, M Guicherd, G Cioci, A Marty and V Kitpreechavanich. Enhancement of poly (L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly (L-lactide) polymer. Polym. Degrad. Stabil. 2017; 143, 64 -73.
T Lomthong, A Areesirisuk, S Suphan, T Panyachanakul, S Krajangsang and V Kitpreechavanich. Solid state fermentation for poly (L-lactide)-degrading enzyme production by Laceyella sacchari LP175 in aerated tray reactor and its hydrolysis of poly (lactide) polymer. Agr. Nat. Resour. 2021; 55, 147-52.
T Lomthong, S Chotineeranat and V Kitpreechavanich. Production and characterization of raw starch degrading enzyme from a newly isolated thermophilic filamentous bacterium, Laceyella sacchari LP175. Starch/Stärke 2015; 67, 255-66.
A Jarerat, Y Tokiwa and H Tanaka. Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly (L-lactide). Appl. Microbiol. Biotechnol. 2006; 72, 726-31.
T Lomthong, S Hanphakphoom, R Yoksan and V Kitpreechavanich. Co-production of poly(L-lactide)-degrading enzyme and raw starch-degrading enzyme by Laceyella sacchari LP175 using agricultural products as substrate, and their efficiency on biodegradation of poly(L-lactide)/thermoplastic starch blend film. Int. Biodeterior. Biodegrad. 2015; 104, 401-10.
M Nadeem, JI Qazi and S Baig. Effect of aeration and agitation rates on alkaline protease production by Bacillus licheniformis UV-9 mutant. Turk. J. Biochem. 2009; 34, 89-96.
DA Goda, AR Bassiouny, NMA Monem, NA Soliman and YRA Fattah. Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH). J. Genet. Eng. Biotechnol. 2020; 18, 23.
AK Urbanek, AM Mironczuk, A Garcia-Martin, A Saborido, IDL Mata and M Arroyo. Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics. Biochim. Biophys. Acta Protein Proteonomics 2020; 1868, 140315.
HA Lim, T Raku and Y Tokiwa. Hydrolysis of polyesters by serine proteases. Biotechnol. Lett. 2005; 27, 459-64.
T Panyachanakul, B Sorachart, S Lumyong, W Lorliam, V Kitpreechavanich and S Krajangsang. Development of biodegradation process for poly (DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1. Electron. J. Biotechnol. 2019; 40, 52-7.
T Lomthong, R Yoksan, S Lumyong and V Kitpreechavanich. Poly(l-lactide)-degrading enzyme production by Laceyella sacchari LP175 under solid state fermentation using low cost agricultural crops and its hydrolysis of poly(l-lactide) film. Waste Biomass Valori. 2020; 11, 1961-70.
A Jarerat and Y Tokiwa. Degradation of poly(l-lactide) by a Fungus. Macromol. Biosci. 2001; 1, 136-40.
SS Umare, AS Chandure and RA Pandey. Synthesis, characterization and biodegradable studies of 1, 3-propanediol based polyesters. Polymer Degrad. Stabil. 2007; 92, 464-79.
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