Microstructural and Protein Subunit Analysis: The Effect of Protease from Chayote, Pineapple Peel and Biduri Leaves as Natural Tenderizer on Mutton, Beef and Buff
DOI:
https://doi.org/10.48048/tis.2023.6519Keywords:
Enzymatic processes, Meat tenderness, Microstructural, Protease, Protein profilesAbstract
Mutton, buffalo and beef meats have a tough texture and sometime is hard to eat. Naturally, enzymatic processing using plant-based compound, including, chayote (Sechium edule), pineapple (Ananas comosus) and biduri (Calotropis gigantea) contains abundance protease that potential to be developed. This research aims to compare the effect chayote, pineapple peel and biduri leaves to tenderize mutton, beef and buff meat at various doses. The sample meats, mutton, beef and buff were obtained from upper leg (round) part. The chayote, pineapple peel powder and biduri leaves was processed into powder and used to smear meats surface with the concentration of 5, 10 and 15 % of natural tenderizers powder from total meat wight (w/w), for 60 min. After treatment the Barford protein analysis using Bio-Rad protein assay was conducted to measure protein concentration, continued with SDS-PAGE analysis using Laemlli method. The structural observation was performed using Scanning Electron Microscope (SEM). The result performs hydrolysis process using pineapple and biduri leaves on mutton and beef produce light protein bands below 26, 34 and between 55 to 43 kDa. The application of natural tenderizer, including pineapple peel, biduri leaf and chayote peel is effectively tenderize the mutton, beef and buff meat. Furthermore, the pineapple peel and biduri leaves powder are the most potential tenderizer for mutton and beef. However, buff is most least tenderized meat among other and it may need an extra dosage or incubation period to get appropriate result.
HIGHLIGHTS
- Natural protease can be extracted from abundance and economical material that potential used for tenderizing mutton, buffalo and beef meat
- Chayote, pineapple peel and biduri leaf contain high protease for natural tenderizer
- The higher natural tenderizer, the higher short chain peptide released from the meat, but it might result in taste change of the meat
- High proteases found in chayote fruit, pineapple fruit skin, and biduri leaf
- The novelty in this research is waste-based protease sources and its application for tenderizing mutton, buffalo and beef, then microstructural analysis and protein profiles are used to determine the effectiveness of the tenderizing meat process
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YH Hwang, A Bakhsh JG Lee and ST Joo. Differences in muscle fiber characteristics and meat quality by muscle type and age of Korean native black goat. Food. Sci. Anim. Resour. 2019; 39, 988-99.
S Kumar and S Yadav. Male buffalo calf: Potential emerging meat animal - a review. J. Anim. Res. 2021; 11, 223-9.
KW Lee, YH Hwang and ST Joo. Meat tenderness characteristics of ten major muscles from hanwoo steers according to quality grades of carcasses. Korean J. Food Sci. Anim. Resour. 2017; 37, 593-8.
A Listrat, M Gagaoua, D Andueza, D Gruffat, J Normand, G Mairesse, B Picard and JF Hocquette. What are the drivers of beef sensory quality using metadata of intramuscular connective tissue, fatty acids and muscle fiber characteristics? Livest. Sci. 2020; 240, 104209.
I Gómez, R Janardhanan, FC Ibañez and MJ Beriain. The effects of processing and preservation technologies on meat quality: Sensory and nutritional aspects. Foods 2020; 9, 1416.
MF Fortes, FHM Leite, JMF Garzillo and CA Monteiro. Analysis of the impact of the meat supply chain on the Brazilian agri-food system. SSRN Electr. J. 2021. DOI: 10.2139/ssrn.3848965.
C Mosnier, A Jarousse, P Madrange, J Balouzat, M Guillier, G Pirlo, A Mertens, E ORiordan, C Pahmeyer, S Hennart, L Legein, P Crosson, M Kearney, P Dimon, C Bertozzi, E Reding, M Iacurto, J Breen, S Carè and P Veysset. Evaluation of the contribution of 16 European beef production systems to food security. Agric. Syst. 2021; 190, 103088.
DL Michalk, DR Kemp, WB Badgery, J Wu, Y Zhang and PJ Thomassin. Sustainability and future food security - a global perspective for livestock production. Land Degrad. Dev. 2019; 30, 561-73.
EN Ponnampalam, AED Bekhit, H Bruce, ND Scollan, V Muchenje, P Silva and JL Jacobs. Production strategies and processing systems of meat: Current status and future outlook for innovation - a global perspective. In: Sustainable meat production and processing. Academic Press, London, 2019, p. 17-44.
N Terjung, F Witte and V Heinz. The dry aged beef paradox: Why dry aging is sometimes not better than wet aging. Meat Sci. 2021; 172, 108355.
S Ryu, MR Park, BE Maburutse, WJ Lee, DJ Park, S Cho, I Hwang, S Oh and Y Kim. Diversity and characteristics of the meat microbiological community on dry aged beef. J. Microbiol. Biotechnol. 2018; 28, 105-8.
EF Vieira, O Pinho, IMPLVO Ferreira and C Delerue-Matos. Chayote (Sechium edule): A review of nutritional composition, bioactivities and potential applications. Food Chem. 2019; 275, 557-68.
A Ikram, S Ambreen, A Tahseen, A Azhar, K Tariq, T Liaqat, MBB Zahid, MA Rahim, W Khalid and N Nasir. Meat tenderization through plant proteases - a mini review. Int. J. Biosci. 2021; 18, 102-12.
A Kaur, DR Batish, S Kaur and BS Chauhan. An overview of the characteristics and potential of Calotropis procera from botanical, ecological, and economic perspectives. Front. Plant Sci. 2021; 12, 690806.
K Ratnayani, AAAS Juwarni, AAIAM Laksmiwati and IGAKSP Dewi. Test the protease activity of chayote and taro sap and their comparison to papaya latex (in Indonesian). J. Kim. 2015; 9, 147-52.
TA Singh, PK Sarangi and NJ Singh. Tenderisation of meat by bromelain enzyme extracted from pineapple wastes. Int. J. Curr. Microbiol. Appl. Sci. 2018; 7, 3256-64.
J Manohar, R Gayathri and V Vishnupriya. Tenderisation of meat using bromelain from pineapple extract. Int. J. Pharm. Sci. Rev. Res. 2016; 39, 81-5.
YN Fawzya and E Chasanah. Application of local proteases to produce fish protein hydrolysate (FPH) in Indonesia. In: Encyclopedia of marine biotechnology. Wiley-Blackwell, Hoboken, New Jersey, 2020, p. 2797-812.
A Pepe, MG Guevara and FR Tito. Potential use of plant proteolytic enzymes in hemostasis. In: MG Guevara and GR Daleo. (Eds.). Biotechnological applications of plant proteolytic enzymes. Springer Nature, Cham, Switzerland, 2018, p. 1-146.
Y Chen, H Li, Y Shen, C Zhang, X Kong, X Li and Y Hua. Endopeptidases, exopeptidases, and glutamate decarboxylase in soybean water extract and their in vitro activity. Food Chem. 2021; 360, 130026.
Y Chen, H Zhang, C Zhang, X Kong and Y Hua. Characterization of endogenous endopeptidases and exopeptidases and application for the limited hydrolysis of peanut proteins. Food Chem. 2021; 345, 128764.
IM Vikhlyantsev and ZA Podlubnaya. Nuances of electrophoresis study of titin/connectin. Biophys. Rev. 2017; 9, 189-99.
AI Kartika, HS Kusuma, S Darmawati and DS Tanjung. Microstructural and proteomic analysis to investigate the effectiveness of papaya leaf as a tenderizer of beef and goat’s meat. IOP Conf. Ser.: Earth Environ. Sci. 2019; 292, 012010.
HK Mæhr, L Dalheim, GK Edvinsen, EO Elvevoll and IJ Jensen. Protein determination-method matters. Foods 2018; 7, 5.
A Mehata and D Dahari. Bradford assay as a high-throughput bioanalytical screening method for conforming pathophysiological state of the animal. J. Drug Deliv. Ther. 2020; 10, 105-10.
UK Laemmli. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227, 680-5.
R Syahbana. 2017, Pemanfaatan enzim bromelin yang diisolasi dari bonggol nanas sebagai pengempuk daging sapi [Utilization of bromelain enzyme isolated from pineapple hump as beef tenderizer] (in Indonesian). Master Thesis. Universsitas Sumatera Utara, Sumatera Utara, Indonesia.
B Konuklugil and O Kuşdemir. Bromelain. J. Lit. Pharm. Sci. 2018; 7, 1-10.
ER Coscueta, ME Brassesco and M Pintado. Collagen-based bioactive bromelain hydrolysate from salt-cured cod skin. Appl. Sci. 2021; 11, 8538.
IC Renu and BSH Thampi. Studies on collagen hydrolysis by pineapple (Ananas comosus) stem bromelain. Int. J. Pharm. Biol. Sci. 2019; 9, 118-26.
TL Margoutomo, BS Hertanto, W Swastike, M Cahyadi, LR Kartikasari and AMP Nuhriawangsa. The quality of skim milk curd produced using biduri (Calotropis gigantea) latex as rennet replacement. IOP Conf. Ser.: Earth Environ. Sci. 2019; 387, 012046.
Y Witono, I Taruna, M Maryanto, RR Fauziah and WS Wahyuningtyas. Antioxidant activity of protein hydrolysates derived from javanese freshwater fishes. In: Proceedings of the 4th International Conference on Food and Agriculture Resources, Yogyakarta, Indonesia. 2018, p. 214-8.
SY Güven, M Karakaya and AS Babaoğlu. Proximate compositions, fatty acids and mineral contents of chicken and turkey gizzards, and textural properties after tenderization with proteolytic enzymes. J. Food Process. Preserv. 2021; 45, e15775.
GDMP Madhusankha and RCN Thilakarathna. Meat tenderization mechanism and the impact of plant exogenous proteases: A review. Arab. J. Chem. 2021; 14, 102967.
H Rizqiati, A Nugraheni, S Susanti, L Fatmawati, N Nuryanto and F Arifan. Characteristic of isolated crude bromelain extract from cayenne pineapple crown in various drying temperature and its effect on meat texture. Food Res. 2021; 5, 72-9.
S Rawdkuen, M Jaimakreu and S Benjakul. Physicochemical properties and tenderness of meat samples using proteolytic extract from Calotropis procera latex. Food Chem. 2013; 136, 909-16.
N Amini, S Setiasih, S Handayani, S Hudiyono and E Saepudin. Potential antibacterial activity of partial purified bromelain from pineapple core extracts using acetone and ammonium sulphate against dental caries-causing bacteria. AIP Conf. Proc. 2018; 2023, 020071.
ANM Ramli, NHA Manas, AAA Hamid, HA Hamid and RM Illias. Comparative structural analysis of fruit and stem bromelain from Ananas comosus. Food Chem. 2018; 266, 183-91.
MZR Silva, JPB Oliveira, MV Ramos, DF Farias, CAD Sá, JAC Ribeiro, AFB Silva, JSD Sousa, RA Zambelli, ACD Silva, GP Furtado, TB Grangeiro, MS Vasconcelos, SR Silveira and CDT Freitas. Biotechnological potential of a cysteine protease (CpCP3) from Calotropis procera latex for cheesemaking. Food Chem. 2020; 307, 125574.
K Ratnayani and NWL Kusumaningrum. Isolation of protease enzyme from chayote fruit (Sechium Edule (Jacq.) Sw.) with ammonium sulfate fractination method. Int. J. Biosci. Biotechnol. 2015; 2, 1-6.
N Takio, M Yadav, M Barman and HS Yadav. Purification, characterization, immobilization and kinetic studies of catalase from a novel source Sechium edule. Int. J. Chem. Kinet. 2021; 53, 596-610.
M Clemente, MG Corigliano, SA Pariani, EF Sánchez-López, VA Sander and VA Ramos-Duarte. Plant serine protease inhibitors: Biotechnology application in agriculture and molecular farming. Int. J. Mol. Sci. 2019; 20, 1345.
LC Soewarlan. Potensi alergi akibat infeksi Anisakis typica pada daging ikan cakalang [Potential allergies due to infection of Anisakis typica on skipjack tuna meat] (in Indonesian). J. Teknol. Ind. Pangan 2016; 27, 200-7.
PP Purslow and JA Trotter. The morphology and mechanical properties of endomysium in series-fibred muscles: Variations with muscle length. J. Muscle Res. Cell Motil. 1994; 15, 299-308.
SR Kanatt, SP Chawla and A Sharma. Effect of radiation processing on meat tenderisation. Radiat Phys. Chem. 2015; 111, 1-8.
I Guerrero-Legarreta, F Napolitano, R Cruz-Monterrosa, D Mota-Rojas, P Mora-Medina, E Ramírez-Bribiesca, A Bertoni, J Berdugo-Gutiérrez and A Braghieri. River buffalo meat production and quality: Sustainability, productivity, nutritional and sensory properties. J. Buffalo Sci. 2020; 9, 159-69.
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