Improving Quality of Sunn Hemp (Crotalaria Juncea L.) Foliage as Roughage Source for Ruminants by using Microorganisms
DOI:
https://doi.org/10.48048/tis.2021.1440Keywords:
Sunn hemp, Bacillus subtilis, Lactic acid bacteriaAbstract
This study aims to investigate the chemical composition, fermentation characteristics, and in vitro ruminal digestibility efficiency of Sunn hemp silage with Fermented juice of epiphytic lactic acid bacteria (FJLAB) and bacillus subtilis. The experiment was designed in a CRD. Five dietary treatments were fresh Sunn hemp (FS, the positive control), Sunn hemp silage (SS, the negative control), Sunn hemp silage with B. subtilis (SSB), Sunn hemp silage with FJLAB (SSL), and SSB plus FJLAB (SSBL). The results showed the OM content of Sunn hemp silage was decreased (p < 0.05), but fiber contents (NDF, ADF, cellulose, and hemicellulose) were increased when compared with fresh Sunn hemp. However, SSL and SSBL could improve nutrition values (higher CP Reduction efficiency; p < 0.01, decrease cellulose; p < 0.01, and hemicellulose content; p < 0.10) and quality grading of Sunn hemp silage when compare with the negative control which did not affect to CP and EE values. Furthermore, FJLAB reduced fiber content and increase CP content of Sunn hemp silage, whereas B. subtilis presented the opposite results. However, the combination of FJLAB and B. subtilis showed the best treatment of Sunn hemp silage of this experiment (the highest CP and EE Reduction efficiency, ruminal gas production, and organic matter degradability; OMD).
HIGHLIGHTS
- Pyrrolizidine Alkaloids, a toxin associated with disease in ruminants, found in Sunn hemp is completely destroyed by anaerobic microorganisms of silage
- The fermentation process of silage was improved by increasing lactic acid production and decreasing pH value to inhibit the growth of undesirable microbes
- Combination of fermented juice of epiphytic lactic acid bacteria (FJLAB) and Bacillus subtilis, lactic acid producer, for Sunn hemp silage treatment yielded the highest lactic acid production and nutritive values
GRAPHICAL ABSTRACT
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R Ramsey, D Doye, C Ward, J McGrann, L Falconer and S Bevers. Factors affecting beef cow her costs production and profits. J. Agric. Appl. Econ. 2005; 37, 91-9.
L Connolly, A Kinsella, G Quinlan and B Moran. National farm survey 2008. Teagasc, Republic of Ireland, 2010, p. 104.
L Shalloo, P Dillon, M Rath and M Wallace. Description and validation of the Moorepark dairy system model. Int. J. Dairy Sci. 2004; 87, 1945-59.
MO Lawal, AZ Aderolu, B Adeyemi and OO Aarode. Dietary effects of Sun-hemp (Crotalaria juncea Linn.) in the diet of African catfish, Clarias gariepinus juveniles. Acta SATECH. 2013; 4, 108-18.
K Janardhanan, V Vadivel and M Pugalenthi. Biodiversity in Indian underexploited/tribal pulses. In: PK Jaiwal and RP Singh (Eds.). Improvement strategies for Leguminosae biotechnology. Kluwer Academic Publishers, Amsterdam, The Netherlands. 2003, p. 353-405.
MO Lawal, AZ Aderolu, B Adeyemi and OO Aarode. Dietary effects of Sun-hemp (Crotalaria juncea Linn.) in the diet of African catfish, Clarias gariepinus juveniles. Acta SATECH. 2013; 4, 108-18.
J Fraser, D McCartney, H Najda and Z Mir. Yield potential and forage quality of annual forage legumes in South Alberta and Northeast Saskatchewan. Can. J. Plant Sci. 2004; 84, 143-55.
Z Mansoer, DW Reeves and CW Wood. Suitability of Sunn hemp as an alternative late-summer legume cover crop. Soil Sci. Soc. Am. J. 1997; 61, 246-53.
PL Sherasia, MR Garg, BT Phondba and SA Hossain. Estimation of metabolizable energy, net energy-lactation and total digestible nutrients of some ruminant feedstuffs using in vitro gas production technique. Indian J. Dairy Sci. 2015; 68, 370-5.
P Dhan, PN Mishra and PS Mishra. Amino acid profile of winged bean (Psophocarpus tetragonolobus (L.). DC: A rich source of vegetable protein. Plant Foods Hum. Nutr. 1987; 37, 261-4.
European Food Safety Authority. Opinion of the scientific panel on contaminants in the food chain on a request from the European commission related to pyrrolizidine alkaloids as undesirable substances in animal feed. EFSA J. 2007; 447, 1-51.
P Lounglawan and W Suksombat. Utilization of sunnhemp meal in beef cattle diet. Research report. Suranaree University of Technology, Thailand. 2015, p. 48.
P Mulder, B Beumer, E Oosterink and JD Jong. Dutch survey pyrrolizidine alkaloids in animal forage. RIKILT-Institute of Food Safety, Wageningen University & Research Centre, The Netherlands. 2009, p. 45.
U Candrian, J Lüthy, P Schmid, C Schlatter and E Gallasz. Stability of pyrrolizidine alkaloids in hay and silage. J. Agric. Food Chem. 1984; 32, 935-7.
E Saarisalo, E Skyttä, A Haikara, T Jalava and S Jaakkola. Screening and selection of lactic acid bacteria strains suitable for ensiling grass. J. Appl. Microbiol. 2007; 102, 327-36.
H Alhaag, X Yuan, A Mala, J Bai and T Shao. Fermentation characteristics of lactobacillus Plantarum and Pediococcus species isolated from sweet sorghum silage and their application as silage inoculants. Appl. Sci. 2019; 9, 1247.
M Kizilsimsek, RJ Schmidt, LJR Kung. Effects of a mixture of lactic acid bacteria applied as a freeze-dried or fresh culture on the fermentation of alfalfa silage. J. Dairy Sci. 2007; 90, 5698-705.
EC Lara, FC Bassoa, FBD Assis, FA Souza, TT Berchielli and RA Reis. Changes in the nutritive value and aerobic stability of corn silages inoculated with Bacillus subtilis alone or combined with Lactobacillus plantarum. Anim. Prod. Sci. 2015; 56, 1867-74.
S Mohd-Setapar, N Abd-Talib and R Aziz. Review on crucial parameters of silage quality. APCBEE Procedia 2012; 3, 99-103.
E Salvucci, JG LeBlanc and G Pérez. Technological properties of lactic acid bacteria isolated from raw cereal material. LWT Food Sci. Technol. 2016; 70, 185-91.
MO Dalheim, NB Arnfinnsdottir, G Widmalm and BE Christensen. The size and shape of three water-soluble, non-ionic polysaccharides produced by lactic acid bacteria: A comparative study. Carbohydr. Polym. 2016; 142, 91-7.
FE Contreras-Govea, RE Muck, GA Broderick and PJ Weimer. Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield. Anim. Feed Sci. Technol. 2013; 179, 61-8.
VL Nsereko, BK Smiley, WM Rutherford, A Spielbauer, KJ Forrester and GH Hettinger. Influence of inoculating forage with lactic acid bacterial strains that produce ferulate esterase on ensilage and ruminal degradation of fiber. Anim. Feed Sci. Technol. 2008; 145, 122-35.
ZG Weinberg, RE Muck and PJ Weimer. The survival of silage inoculant lactic acid bacteria in rumen fluid. J. Appl. Microbiol. 2003; 94, 1066-71.
ZG Weinberg, Y Chen and M Gamburg. The passage of lactic acid bacteria from silage into rumen fluid, in vitro studies. J. Dairy Sci. 2004; 87, 3386-97.
RE Muck, I Filya and FE Contreras-Govea. Inoculant effects on alfalfa silage: In vitro gas and volatile fatty acid production. J. Dairy Sci. 2007; 90, 5115-25.
G Pahlow, RE Muck, F Driehuis, S Elferink and SF Spoelstra. Microbiology of ensiling. Agronomy. 2003; 42, 31-94.
LE Phillip and V Fellner. Effects of bacterial inoculation of high-moisture ear corn on its aerobic stability, digestion, and utilization for growth by beef steers. J. Anim. Sci. 1992; 70, 3178-87.
TA McAllister, LB Selinger, LR McMahon, HD Bae, TJ Lysyk, SJ Oosting and KJ Cheng. Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of Lactobaciollus plantarum and Enterococcus faecium. Can. J. Anim. Sci. 1995; 75, 425-32.
RE Muck. Effects of corn silage inoculants on aerobic stability. Trans ASAE. 2004; 47, 1011-6.
H Zahiroddini, J Baah, W Absalom and TT McAlister. Effect of an inoculant and hydrolytic enzymes on fermentation and nutritive value of whole crop barley silage. Anim. Feed Sci. Technol. 2004; 117, 317-30.
Association of Official Analytical Chemists (AOAC). The association of official analytical chemists. 17th ed. Official Analytical Chemists, Arlington, Virginia, 2005.
PJ Van Soest, JB Robertson and BA Lewis. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 1991; 74, 3583-97.
S Bureenok, W Suksombat and Y Kawamoto. Effects of the fermented juice of epiphytic lactic acid bacteria (FJLB) and molasses on digestibility and rumen fermentation characteristics of ruzigrass (Brachiaria ruziziensis) silages. Livestock Sci. 2011; 138, 266-71.
LJR Kung, RD Shaver, RJ Grant and RJ Schmidt. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. J. Dairy Sci. 2018; 101, 4020-33.
Department of Livestock Development. Standard guide of silage. The agricultural Co-operative fereration of Thailand, LTD., Thailand, 2004, p. 23.
KH Menke and H Steingass. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Anim. Res. Dev. 1988; 28, 7-55.
ER Ørskov and I McDonald. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agri. Sci. 1979; 92, 499-503.
I McDonald. A revised model for the estimation of protein degradability in the rumen. J. Agric. Sci. 1981; 96, 251-2.
S Mathew, S Sagathewan, J Thomas and G Mathen. An HPLC method for estimation of volatile fatty acids of ruminal fluid. Indian J. Anim. Sci. 1997; 67, 805-7.
M Woolford and G Pathow. The silage Fermentation. Microbiology of Fermented Foods, Blackie Academic and Professional, London, UK. 1997, p. 73-102.
RY Leibensperger and RE Pitt. Modelling the effects of formic acid and molasses on ensilage. J. Dairy Sci. 1988; 71, 1220-31.
MD Flythe and JB Russel. The effect of pH and a bacteriocin (bovicin HC5) on Clostridium sporogenes MD1, a bacterium that has the ability to degrade amino acids in ensiled plant materials. FEMS Microbiol. Ecol. 2004; 47, 215-22.
G Borreani, E Tabacco, RJ Schmidt, BJ Holmes and RE Muck. Silage review: Factors affecting dry matter and quality losses in silages. J. Dairy Sci. 2018; 101, 3952-79.
E Tabacco, F Righi, A Quarantelli and G Borreani. Dry matter and nutritional losses during aerobic deterioration of corn and sorghum silages as influenced by different lactic acid bacteria inocula. J. Dairy Sci. 2011; 94, 1409-19.
LJR Kung, AC Sheperd, AM Smagala, KM Endres, CA Bessett, NK Ranjit and JL Glancey. The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. J. Dairy Sci. 1998; 81, 1322-30.
DH Kleinschmit and LJR Kung. A meta-analysis of the effects of Lactobacillus buchneri on the fermentation and aerobic stability of corn and grass and small-grain silages. J. Dairy Sci. 2006; 89, 4005-13.
MR Bedford and GG Partridge. Enzymes in Farm Animal Nutrition. CABI Publishing, New York. 2001, p. 406.
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