Modulatory Effects of Brassica rapa Polysaccharides on Metabolic Obesity and Blood Biochemistry in Experimental Models of Diabetes and Hyperlipidemia

Authors

  • Dilnoza Abdulgafurova A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Dilfuza Amanlikova A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Lazizbek Makhmudov A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Nodira Obidova A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Munojat Oripova A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Zulfizar Kuziyeva A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan
  • Yuliya Oshchepkova A. S. Sadykov Institute of Bioorganic Chemistry of the Science Academy of Uzbekistan “Laboratory of Plant Cytoprotectors, Chemistry of Proteins and Peptides Laboratory” and “Pharamacology,” Tashkent, Uzbekistan

DOI:

https://doi.org/10.48048/tis.2026.11320

Keywords:

Brassica rapa, Polysaccharides, Alloxan diabetes, Hyperlipidemia, Body weight, ALT, ACT, Glucosidase, Amylase, Biochemical parameters, Pancreas, Digestive enzymes, Rats

Abstract

Polysaccharides extracted from Brassica rapa (turnip) seeds were investigated for their potential hypolipidemic and hypoglycemic effects in rat models of hyperlipidemia and alloxan-induced diabetes mellitus. The polysaccharides were obtained through hot water extraction and ethanol precipitation, followed by deproteinization, ion-exchange chromatography, and gel filtration. Chemical characterization by GC/MS and IR spectroscopy revealed that the dominant monosaccharides were arabinose and galactose, indicating an arabinogalactan structure. In vivo experiments showed that oral administration of Brassica rapa polysaccharides (BRP) significantly reduced blood glucose levels, total cholesterol, triglycerides, and LDL-C, while increasing HDL-C and total protein. BRP also normalized ALT and AST activities and improved the activity of digestive enzymes such as amylase and glucosidase. Correlation analysis revealed strong positive associations between body weight and triglycerides (r = 0.78), and between glucose and amylase activity (r = 0.69), indicating the polysaccharides’ role in modulating lipid and carbohydrate metabolism. The histological and biochemical improvements observed in treated animals suggest that BRP exerts its beneficial effects through multiple mechanisms, including antioxidant, anti-inflammatory, and enzyme-modulating activities. These findings support the therapeutic potential of Brassica rapa polysaccharides as functional agents for the management of metabolic disorders such as type 2 diabetes and hyperlipidemia.

HIGHLIGHTS

  • Brassica rapa polysaccharides at a dose of 25 mg/kg reduce serum glucose levels in animals with alloxan-induced diabetes.
  • Brassica rapa polysaccharides at a dose of 25 mg/kg normalize body weight and partially restore the lipid profile of animals with hyperlipidemia.
  • Increasedordecreasedlevels of enzymes(glucosidaseandalpha-amylase)under the action of Brassica rapa at a dose of 25 mg /kg affect the course of biochemical metabolic processes.
  • Brassica rapa polysaccharides at a dose of 25 mg/kg modulate both carbohydrate and lipid metabolism.
  • Against the background of the induction of metabolic disorders, the relationship between the development of hyperglycemia and dyslipidemia is manifested.

GRAPHICAL ABSTRACT

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Published

2025-10-10

Issue

Vol. 23 No. 1 (2026): Trends in Sciences, Volume 23, Number 1, January 2026

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Research Articles

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