Antibacterial Hydrogel Films Embedded with Cu–Ag Oxide Nanoparticles Based on Basil Seed Mucilage for Advanced Wound Dressing Applications

Authors

  • Jiyapa Sripirom Department of Chemistry, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand
  • Rungrudee Srisomang Department of Chemistry, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand
  • Sakuntala Siri-Udom Department of Biology, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand
  • Cybelle Morales Futalan Institute of Civil Engineering, University of the Philippines Diliman, Quezon City 1101, Philippines
  • Khongvit Prasitnok Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Orrasa Prasitnok Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Sirilak Kamonwannasit Department of Agro-Industrial Product Development, Faculty of Agricultural Technology, Burapha University, Sakaeo 27160, Thailand
  • Agarat Kamcharoen Department of Agro-Industrial Product Development, Faculty of Agricultural Technology, Burapha University, Sakaeo 27160, Thailand
  • Pongtanawat Khemthong National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand
  • Piaw Phatai Department of Chemistry, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand

DOI:

https://doi.org/10.48048/tis.2025.10418

Keywords:

Alginate, Antibacterial activity, Basil seed mucilage, Composite material, Copper-silver oxide, Gelatin, Hydrogel film, Alginate, Antibacterial activity, Basil seed mucilage, Composite material, Copper-silver oxide, Gelatin, Hydrogel film

Abstract

Hydrogel films with antibacterial properties, swelling capability, and mechanical strength show promise as wound dressings. This study investigates the fabrication of Cu1.0-xAgₓO (x = 0.0, 0.1, 0.5, 0.9, and 1.0) antibacterial nanoparticles loaded into basil seed mucilage (BSM)/hydrogel (BSM/HG) films via a casting method. The crystal phases, surface chemical composition, and antimicrobial efficacy of Cu1.0-xAgₓO particles were characterized using X-ray diffraction (XRD), X-ray photoemission spectroscopy (XPS), and agar well diffusion assays. XRD analysis confirmed that
Cu1.0-xAgₓO (x = 0.1 – 0.7) samples formed composites containing CuO, Ag, and Ag2O phases. Among them, Cu0.7Ag0.3O exhibited superior antibacterial activity, achieving inhibition zone diameters of 8 - 13 mm against Bacillus cereus, Staphylococcus aureus, Streptococcus thermophilus, and Escherichia coli. The hydrogel demonstrated good swelling capacity and mechanical strength suitable for wound dressing applications. The incorporation of Cu0.7Ag0.3O nanoparticles into BSM/HG films reduced both swelling capacity and tensile strength due to particle agglomeration, although the antibacterial properties were significantly enhanced. This research highlights the potential of Cu1.0-xAgₓO-loaded BSM/HG films for advanced wound dressing applications.

HIGHLIGHTS

  • Formation of various phases on Cu0.7Ag0.3O composite including CuO, metallic Ag and Ag2O improved antibacterial activity.
  • Cu0.7Ag0.3O loaded BSM/HG film showed excellent antibacterial activity against Gram-positive and Gram-negative bacteria.
  • Cu0.7Ag0.3O loaded BSM/HG film has the suitability as an antibacterial material in biomedical applications.

GRAPHICAL ABSTRACT

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Published

2025-07-15

Issue

Vol. 22 No. 10 (2025): Trends in Sciences, Volume 22, Number 10, October 2025

Section

Research Articles

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