Validation of Wavelength-Dispersive X-Ray Fluorescence Technique for Rapid Screening Iron and Zinc Concentration in Rice Seed

Authors

  • Piyanuch Orpong Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Kanokporn Boonsirichai Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Roppon Picha Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Ratchai Funklin Nuclear Technology Service Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Supalak Kongsri Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Chunyapuk Kukusamude Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
  • Sasiwimon Naksuriyawong Nuclear Technology Service Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand

DOI:

https://doi.org/10.48048/tis.2025.9068

Keywords:

Iron, Zinc, Method validation, WD-XRF, Rice, Rapid screening, Calibration

Abstract

This study focuses on the validation of a wavelength dispersive X-ray fluorescence (WD-XRF) spectrometer for the rapid determination of iron (Fe) and zinc (Zn) concentrations in certified reference materials (CRMs). Five CRMs were used to establish calibration curves. The results were analyzed in terms of accuracy, precision, repeatability and linearity to assess the performance of the WD-XRF method. Accuracy was evaluated by comparing measured values to certified values, with relative accuracy calculated for each sample. Precision was determined through repeatability, where samples were measured in at least 7 replicates across different days, and relative standard deviation (RSD) was calculated. Linearity was assessed by plotting measured intensities and concentrations against certified concentrations, with the correlation coefficient (R2) values close to 1, indicating a strong linear relationship. Additionally, the WD-XRF method demonstrated high repeatability, with low RSD values across multiple measurements. The study concluded that WD-XRF is a reliable method for the rapid analysis of elemental composition, providing strong accuracy, precision and linearity for Fe and Zn determination in CRMs and can be applied as a valuable method for rapid and non-destructive analysis in various agricultural and food-related applications.

HIGHLIGHTS

  • The effectiveness of wavelength dispersive X-ray fluorescence (WD-XRF) method was evaluated to confirm its reliability for determining the elemental composition, specifically iron (Fe) and zinc (Zn), in plant-based certified reference materials (CRMs).
  • Five CRMs were used for calibration and 2 known samples were used as the test samples to determine the analytical performance of WD-XRF.
  • The linearity and correlation with the certified values of WD-XRF results revealed strong correlation with R-square being greater than 0.95 and the recovery percentage of 2 known samples were range from 98.75 - 109.66 for Fe and Zn concentration.
  • WD-XRF spectrometer can be used as a rapid method for determination of Fe and Zn concentrations in rice seeds.

GRAPHICAL ABSTRACT

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Published

2024-12-10

Issue

Vol. 22 No. 2 (2025): Trends in Sciences, Volume 22, Number 2, February 2025

Section

Research Articles

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