Evaluation of the Phytochemical Profiling, Tyrosinase Inhibitory Activity, and Antioxidant Potential of Various Extracts of Acacia Concinna (Willd.) DC.
DOI:
https://doi.org/10.48048/tis.2024.8334Keywords:
Acacia concinna, Phytochemical profiling, Antioxidant, Tyrosinase inhibitory activityAbstract
The legume Acacia concinna (Willd.) DC. (Fabaceae) is eaten as a food and used for medicinal purposes. This study evaluated the phytochemical profile, total phenolic content and antioxidant and tyrosinase inhibitory activities of leaf, bark, and heartwood extracts of A. concinna using different extraction solvents. Crude extracts were prepared by reflux extraction with dichloromethane, ethanol, and deionized water. The dichloromethane extracts of bark and heartwood provided the highest total phenolic content as determined by the Folin-Ciocalteu reaction. Antioxidant activities were assessed using the DPPH and ABTS assays, with the bark ethanolic extract (BE) giving the highest antioxidant activities at IC50 values of 3.0 ± 0.03 and 5.0 ± 0.01 µg/mL, respectively. The BE antioxidant capacity was similar to ascorbic acid. Tyrosinase inhibitory activity was determined using an in vitro enzymatic spectroscopic model. Highest tyrosinase inhibitory activity was recorded in the heartwood ethanolic extract (HE) with IC50 value 0.7634 ± 0.01 mg/mL. GC-MS analysis revealed long-chain fatty acids as the major phytochemicals in BE and HE. BE had excellent antioxidant abilities, whereas HE showed potential tyrosinase inhibitory activity. Long-chain fatty acids and lupeol were the principal phytoconstituents of BE and HE as important bioactive components in A. concinna extracts.
HIGHLIGHTS
- The bark extracts have the highest total phenolic content and antioxidant activities in comparison to those of heartwood and leaf extracts.
- The heartwood extracts have the highest tyrosinase inhibitory activity.
- 9-octadecanoic acid ethyl ester, hexadecanoic acid and lupeol were the principal phytoconstituents of BE and HE as important bioactive components in the concinna extracts.
GRAPHICAL ABSTRACT
Downloads
References
W Poomanee, W Chaiyana, RR Wickett and P Leelapornpisid. Stability and solubility improvement of Sompoi (Acacia concinna Linn.) pod extract by topical microemulsion. Asian J. Pharm. Sci. 2017; 12, 386-93.
MT Muhammad and MN Khan. Eco-friendly, biodegradable natural surfactant (Acacia concinna): An alternative to the synthetic surfactants. J. Clean. Prod. 2018; 188, 678-85.
IA Shaikh, UM Muddapur, ZK Bagewadi, S Chiniwal, MM Ghoneim, MH Mahnashi, F Alsaikhan, D Yaraguppi and FN Niyonzima. Characterization of bioactive compounds from acacia concinna and citrus limon, silver Nanoparticles’Production by A. concinna extract, and their biological properties. Molecules 2022; 27, 2715.
A Ganogpichayagrai and C Suksaard. Evaluation of proximate composition and biological activities of sompoi (Acacia concinna) leaves in Thailand. J. Adv. Pharm. Tech. Res. 2022; 13, 317-21.
P Jeeno, S Yadoung, P Yana and S Hongsibsong. Phytochemical profiling and antioxidant capacity of traditional plants, Northern Thailand. Plants 2023; 12, 3956.
AM Thawabteh, A Jibreen, D Karaman, A Thawabteh and R Karama. Skin pigmentation types, causes and treatment-a review. Molecules 2023; 28, 4839.
IA Ahmed, MA Mikail, N Zamakshshari and ASH Abdullah. Natural anti-aging skincare: Role and potential. Biogerontology 2020; 21, 293-310.
M Platzer, S Kiese and T Tybussek. Radical scavenging mechanisms of phenolic compounds: Aquantitative structure-property relationship (QSPR) study. Front. Nutr. 2022; 9, 882458.
V Jesumani, H Du, M Aslam, P Pei and N Huang. Potential use of seaweed bioactive compounds in skincare-A review. Mar. Drugs 2019; 17, 688.
L Opperman, MD Kock, J Klaasen and F Rahiman. Tyrosinase and melanogenesis inhibition by indigenous African plants: A review. Cosmetics 2020; 7, 60-73.
AM Muddathir, K Yamauchi, I Batubara, EAM Mohieldin and T Mitsunaga. Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants. S. Afr. J. Bot. 2017; 109, 9-15.
JP Zegarac, S Martinez, LV Zulj, T Stipčević and KK Ganić. A comparison of methods used to define the phenolic content and antioxidant activity of Croatian wines. Food Tech. Biotechnol. 2005; 43, 271-6.
K Likhitwitayawuid, A Sornsute, B Sritularak and P Ploypradith. Chemical transformations of ox resveratrol (trans-2,4,3,5-tetrahydroxystilbene) into a potent tyrosinase inhibitor and a strong cytotoxic agent. Bioorganic. Med. Chem. Lett. 2006; 16, 5650-3.
B Payet, ASC Sing and J Smadja. Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: Determination of their polyphenolic and volatile constituents. J. Agr. Food Chem. 2005; 53, 10074-9.
N Alam, KN Yoon, KR Lee, PG Shin, JC Cheong, YB Yoo, JM Shim, MW Lee, UY Lee and TS Lee. Antioxidant activities and tyrosinase inhibitory effects of different extracts from Pleurotus ostreatus fruiting bodies. Mycobiology 2010; 38, 295-301.
N Suphrom, W Sonyota, K Insumronga, P Sawangsupa, P Sutamuanga and K Ingkaninanb. GC-MS analysis and in vitro anti-androgenic activity of Kaempferia rotunda Linn. extract. Naresuan Univ. J. Sci. Tech. 2017; 4, 34-43.
K Mazumder, A Nabila, A Aktar and A Farahnaky. Bioactive variability and in vitro and in vivo antioxidant activity of unprocessed and processed flour of nine cultivars of Australian lupin Species: A comprehensive substantiation. Antioxidants 2020; 9, 282.
N Kooltheat, A Tedasen, K Yamasaki and M Chatatikun. Melanogenesis inhibitory activity, chemical components and molecular docking studies of Prunus cerasoides Buch.-Ham. D. Don. flowers. J. Evid. Based Integr. Med. 2023; 28, 1-20.
A AlQathama, UF Ezuruike, ALDA Mazzari, A Yonbawi, E Chieli and JM Prieto. Effects of selected Nigerian medicinal plants on the viability, mobility, and multidrug-resistant mechanisms in liver, colon, and skin cancer cell lines. Front. Pharmacol. 2020; 11, 546439.
L Kai, Z Xumin, X Long, D Mao, C Huijuan, S Jiawen, L Jiaying, L Xiaofang and L Jia. Lupeol and its derivatives as anticancer and anti-inflammatory agents: Molecular mechanisms and therapeutic efficacy. Pharmacol. Res. 2021; 164, 105373.
MK Tchimene, C Nwaehujor, MO Ezenwali, CO Okoli and M Iwu. Free radical scavenging activity of lupeol isolated from the methanol leaf extract of Crateva adansonii oliv. (Capparidaceae). Int. J. Pharmacogn. Phytochem. Res. 2016; 8, 419-26.
A Muhammad, M Yau and HM Sirat. Antibacterial and antityrosinase activity of isolated compounds from stem bark of Ficus platyphylla del. Int. J. Adv. Sci. Eng. Tech. 2020; 14, 12-3.
AMO Amoussa, L Lagnika, M Bourjot, C Vonthron-Senecheau and A Sanni. Triterpenoids from Acacia ataxacantha DC: Antimicrobial and antioxidant activities. BMC Complementary Altern. Med. 2016; 16, 284.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Walailak University
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
