Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil
DOI:
https://doi.org/10.48048/tis.2024.7917Keywords:
Acclimatization, Auxin, Cytokinin, In vitro culture, Micropropagation, Multiple shoot regeneration, Plant growth regulators (PGRs), Petiole, Plant regeneration, Plant tissue cultureAbstract
Petiole segments of an economically ornamental plant Oxalis triangularis A.st.-Hil have been used in the present study for rapid, reliable and systematic in vitro propagation. The morphogenic effect of α-Naphthalene acetic acid (NAA) at various concentrations (0.5 - 3.0 mg/L) was studied individually or in combination with 6-benzylaminopurine (BAP). Superior multiplication rates were achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP. Of the tested combinations, maximum shoot regeneration (100.00 %), mean shoot number (14.60 ± 0.37 shoots per segment), and shoot length (18.11 ± 0.30 mm) were recorded on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BAP after 6 weeks of incubation. Half-strength MS medium augmented with 1.0 mg/L indole-3-butyric acid (IBA) was the optimal medium for in vitro root induction, (24.60 ± 0.37 roots per shoot with 9.10 ± 0.32 mm in length) after 6 weeks of incubation. The regenerated plantlets showed 100 % survival success during acclimatization and exhibited normal growth characteristics and morphology. This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil.
HIGHLIGHTS
- The tissue culture technology was successfully employed for developing a practicable simple, efficient, and amenable protocol for mass multiplication of Oxalis triangularis A.st.-Hil, an economically ornamental plant
- Our studies based on interaction and combinations of auxins with BAP provided better shoot proliferation
- The regenerated plantlets survived well under field conditions
- This optimized protocol is cost-effective and highly efficient for the rapid mass propagation of Oxalis triangularis A.st.-Hil
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C Catalano, MD Guardo, G Distefano, M Caruso, E Nicolosi, Z Deng, A Gentile and SGL Malfa. Biotechnological approaches for genetic improvement of lemon (Citrus limon (L.) Burm. f.) against Mal Secco Disease. Plants 2021; 10, 1002.
R Yarra, L Jin, Z Zhao and H Cao. Progress in tissue culture and genetic transformation of oil palm: An overview. Int. J. Mol. Sci. 2019; 20, 5353.
S Weckx, D Inzé and L Maene. Tissue culture of oil palm: Finding the balance between mass propagation and somaclonal variation. Front. Plant Sci. 2019; 10, 722.
S Rittirat, S Klaocheed and K Thammasiri. Large scale in vitro micropropagation of an ornamental plant, Oxalis triangularis A.st.-Hil, for commercial application. Acta Horticulturae 2022; 1339, 245-55.
T Murashige and F Skoog. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 1962; 15, 473-97.
V Verma, G Zinta and K Kanwar. Optimization of efficient direct organogenesis protocol for Punica granatum L. cv. Kandhari Kabuli from mature leaf explants. Vitro Cell. Dev. Biol. Plant 2021; 57, 48-59.
AV Deepa and TD Thomas. High-frequency direct shoot induction from leaf explants of Pogostemon quadrifolius (Benth.) F. Muell.: An ethnomedicinal herb. Vitro Cell. Dev. Biol. Plant 2022; 58, 321-9.
D Miroshnichenko, I Chaban, M Chernobrovkina and S Dolgov. Protocol for efficient regulation of in vitro morphogenesis in einkorn (Triticum monococcum L.), a recalcitrant diploid wheat species. PLos One 2017; 12, e0173533.
TD Salla, CS Silva, KLG Machado, LV Astarita and ER Santarém. Non-aerated liquid culture promotes shoot organogenesis in Eucalyptus globulus Labill. J. Forest. Res. 2018; 29, 623-9.
M Manokari, MC Raj, A Dey, M Faisal, AA Alatar, A Alok and MS Shekhawat. Micro‑morpho‑anatomical changes in leaf structure of plantlets during in vitro propagation (micropropagation) of Gardenia jasminoides J. Ellis. Int. J. Plant Res. 2023, https://doi.org/10.1007/s42535-023-00577-6
M Ghorbanpour, M Omidi, A Etminan, M Hatami and L Shooshtari. In vitro hyoscyamine and scopolamine production of black henbane (Hyoscyamus niger) from shoot tip culture under various plant growth regulators and culture media. Trakia J. Sci. 2013; 2, 125-34.
P Glocke, G Collins and M Sedgley. 6-Benzylamino purine stimulates in vitro shoot organogenesis in Eucalyptus erythronema, E. stricklandii and their interspecifc hybrids. Sci. Horticulturae 2006; 109, 339-44.
S Kumar. A review of ethano-medicinal properties and plant tissue culture of high value solanaceous plants. J. Phytological Res. 2017; 30, 63-75.
C Coenen and TL Lomax. Auxin-cytokinin interactions in higher plants: Old problems and new tools. Trends Plant Sci. 1997; 2, 351-6.
AO Aremu, L Plačková, MW Bairu, O Novák, L Plíhalová, K Doležal, JF Finnie and JV Staden. How does exogenously applied cytokinin type afect growth and endogenous cytokinins in micropropagated Merwilla plumbea? Plant Cell Tissue Organ Cult. 2014; 118, 245-56.
CA Auer, M Laloue, JD Cohen and TJ Cooke. Uptake and metabolism of benzyladenine during shoot organogenesis in Petunia leaf explants. Plant Growth Regul. 1992; 11, 105-14.
Y Cui, Y Deng, K Zheng, X Hu, M Zhu, X Deng and R Xi. An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Sci. Rep. 2019; 9, 9634.
N Kannan, M Manokari and MS Shekhawat. Induction of adventitious roots from leaf explants of Morinda coreia Buch. and ham.: An important dye yielding plant. Plant Cell Tissue Organ Cult. 2021; 145, 457-60.
M Manokari, S Priyadharshini and MS Shekhawat. Infuence of physio‑chemical factors on high throughput plant regeneration and micro‑morpho‑anatomy of shoots of Ormocarpum sennoides (Willd.) DC. Acta Physiologiae Plantarum 2021; 43, 5.
SA Hussain, N Ahmad and M Anis. Synergetic efect of TDZ and BA on minimizing the post‑exposure efects on axillary shoot proliferation and assessment of genetic fidelity in Rauvolfia tetraphylla (L.). Rendiconti Lincei Scienze Fisiche E Naturali 2018; 29, 109-15.
M Mani, CR Mathiyazhagan, P Selvam, M Phulwaria and MS Shekhawat. Foliar micro‑morphology: A promising tool to improve survival percentage of tissue culture raised plantlets with special reference to in vitro propagation of Vitex negundo L. Vegetos 2020; 33, 504-15.
MS Shekhawat and M Manokari. In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): A multipurpose threatened species. Physiol. Mol. Biol. Plants 2016; 22, 131-42.
JATD Silva, A Gulyás, K Magyar‑Tábori, MR Wang, QC Wang and J Dobránszki. In vitro tissue culture of apple and other Malus species: Recent advances and applications. Planta 2019; 249, 975-1006.
A Jamal, G Ayub, A Rahman, A Rashid, J Ali and M Shahab. 08. Effect of IBA (Indole Butyric Acid) levels on the growth and rooting of different cutting types of Clerodendrum splendens. Pure Appl. Biol. 2016; 5, 64-71.
MA Elhaak, MZ Matter, MA Zayed and DA Gad. Propagation principles in using Indole-3-butyric acid for rooting rosemary stem cuttings. J. Horticulture 2015; 2, 1000121.
A Aygun and H Dumanoglu. In vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallas. Front. Plant Sci. 2015; 6, 225.
MN Khanam and M Anis. Organogenesis and efficient in vitro plantlet regeneration from nodal segments of Allamanda cathartica L. using TDZ and ultrasound assisted extraction of quercetin. Plant Cell Tissue Organ Cult. 2018; 134, 241-50.
WT Zhu, JZ Du, HB Sun, SY Jiang, XJ Chen, H Sun, Y Zhou and HL Wang. In vitro propagation of an endemic and endangered medicinal plant Notopterygium incisum. Plant Cell Tissue Organ Cult. 2018; 135, 559-63.
S Rittirat. A reliable protocol for micropropagation of an ornamental aquatic plant, Staurogyne repen. Acta Horticulturae 2022; 1339, 227-36.
S Rittirat, S Klaocheed and K Thammasiri. In vitro shoot organogenesis in Anthurium andraeanum ‘HC 028’ - a highly valued cut-flower and potted ornamental plant. Acta Horticulturae 2022, https://doi.org/10.17660/ActaHortic.2022.1339.36.
SK Choudhary, AK Patel, Harish, S Shekhawat and NS Shekhawat. An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: An underutilized nutraceutical vegetable crop. Physiol. Mol. Biol. Plants 2017; 23, 713-22.
T Kiba and A Krapp. Plant nitrogen acquisition under low availability: Regulation of uptake and root architecture. Plant Cell Physiol. 2016; 57, 707-14.
EM Frick and LC Strader. Roles for IBA-derived auxin in plant development. J. Exp. Bot. 2018; 69, 169-77.
J Pang, Y Xiong, Y Zeng, X Chen, X Zhang, Y Li, K Wu, S Zeng, JATD Silva and G Ma. Shoot organogenesis and plant regeneration from leaf and petiole explants of Corydalis saxicola Bunting. Vitro Cell. Dev. Biol. Plant 2023; 59, 121-8.
A Bishnoi, A Bishnoi and S Sehrawat. Efect of growth regulators on in vitro root formation in strawberry. Res. Environ. Life Sci. 2016; 9, 1316-8.
T Ugandhar, M Venkateshwarlu, G Odelu, T Srilatha and UA Devi. In vitro propagation of Solanum surattense Burm. F by high frequency multiple shoot induction from foral bud explants. Int. J. Curr. Adv. Res. 2017; 6, 8264-8.
M Faisal, N Ahmad, M Anis, AA Alatar and AA Qahtan. Auxin-cytokinin synergism in vitro for producing genetically stable plants of Ruta graveolens using shoot tip meristems. Saudi J. Biol. Sci. 2018; 25, 273-7.
CM Dalavi, J Naravula, PBK Kishor and S Patil. Rapid, reliable plantlet regeneration, hairy root induction and in vitro potential for solasodine alkaloid accumulation in an important medicinal plant Solanum virginianum. Plant Cell Tissue Organ Cult. 2023; 153, 191-204.
J Yu,W Liu, J Liu, P Qin and L Xu. Auxin control of root organogenesis from callus in tissue culture. Front Plant Sci. 2017; 8, 1385.
MS Shekhawat, N Kannan, M Manokari and S Priyadharshini. Regeneration of shoots via direct somatic embryogenesis from the leaf surface of Scaevola taccada (Gaertn.) roxb. - a climate resilient species of coastal areas. S. Afr. J. Bot. 2021; 140, 276-83.
H Slesak, G Goralski, D Kwolek, K Dziedzic and A Grabowska-Joachimiak. Male adventitious roots of Rumex thyrsiflorus Fingerh. as a source of genetically stable micropropagated plantlets. Plant Cell Tissue Organ Cult. 2015; 123, 193-203.
KG Abdelaleem. In vitro organogenesis of (Solanum tuberosum L.) plant cultivar alpha through tuber segment explants callus. Int. J. Curr. Microbiol. App. Sci. 2015; 4, 267-76.
RA Najar, MD Fayaz, MH Bhat, M Bashir, A Kumar and AK Jain. An efcient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica. Biosci. Biotechnol. Res. Comm. 2018; 11, 144-53.
J Revathi, M Manokari and MS Shekhawat. Optimization of factors affecting in vitro regeneration, flowering, ex vitro rooting and foliar micromorphological studies of Oldenlandia corymbosa L.: A multipotent herb. Plant Cell Tissue Organ Cult. 2018; 134, 1-13.
P Silpa and TD Thomas. High‑frequency shoot regeneration from fower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats. Plant Cell Tissue Organ Cult. 2021; 147, 221-8.
J Hou, S Ding, P Su, D Wang and L Wu. Efficient plant regeneration mediated by root segment-derived juvenile shoots of Rhus chinensis Mill. Trees 2023; 37, 1055-67.
J Hou, Y Mao, P Su, D Wang, X Chen, S Huang, J Ni, W Zhao and L Wu. A high throughput plant regeneration system from shoot stems of Sapium sebiferum Roxb., a potential multipurpose bioenergy tree. Ind. Crop. Prod. 2020; 154, 112653.
Q Sun, H Sun, RL Bell and Q Guan. Plant regeneration from in vitro leaf explants of Prunus pseudocerasus Lindl. Propag. Ornamental Plants 2018; 18, 3-11.
Q Sun, M Sun, H Sun, RL Bell, L Li, W Zhang and J Tao. Comparative organogenic response of six clonal apple rootstock cultivars. HortScience 2016; 51, 272-8.
SA Hussain, N Ahmad, M Anis and AA Alatar. Infuence of meta‑topolin on in vitro organogenesis in Tecoma stans L., assessment of genetic fidelity and phytochemical profling of wild and regenerated plants. Plant Cell Tissue Organ Cult. 2019; 138, 339-51.
A Naaz, SA Hussain, M Anis and AA Alatar. Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions. Biol. Plantarum 2019; 63, 174-82.
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