Quantification of Vanillin, Ethyl Vanillin and Methyl Vanillin Among Thai Rice Cultivars by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
DOI:
https://doi.org/10.48048/tis.2024.7252Keywords:
Rice, Vanillin, Ethyl vanillin, Methyl vanillin, LC-MS/MSAbstract
A method was developed and validated for vanillin, ethyl vanillin, and methyl vanillin determination in Thai rice cultivars by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the selected reaction monitoring mode. In rice samples, the limit of detection was 13.3, 11.2 and 5.0 mg/kg for vanillin, ethyl vanillin, and methyl vanillin. A high correlation coefficient was obtained (R2 = 0.9999, 0.9999 and 0.9998) within the linear range (0 - 300 mg/L) in the MS method. The recoveries ranging from 90.63 % ± 6.25 to 108.7 % ± 2.35 were obtained in different spiking concentrations. The average RSD% of intra-day for vanillin, ethyl vanillin, and methyl vanillin was 6.90, 5.13 and 2.16 %, and the inter-day were 6.72, 5.30 and 5.64 %, respectively. The developed method was applied to 24 commercial rice samples. Vanillin, were found in 24 samples ranging from 95.2 to 773.2 mg/kg. However, ethyl vanillin and methyl vanillin were not detected under LOD. The developed UPLC-MS/MS method for vanillin, ethyl vanillin and methyl vanillin determination include the merits of simple preparation steps and high sensitivity.
HIGHLIGHTS
- Method development for analyzing vanillin, ethyl vanillin, and methyl vanillin in rice grain by UPLC-MS/MS.
- High accuracy and precision method for determining vanillin and its derivatives.
- Quantitative analysis of vanillin, ethyl vanillin, and methyl vanillin in Thai rice cultivars.
GRAPHICAL ABSTRACT
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