Molecular Integrity and Antioxidants Status of Nile Tilapia (O. niloticus) Exposed to Indigofera tinctoria
DOI:
https://doi.org/10.48048/tis.2023.5775Keywords:
Indigofera tinctoria, Oxidative stress, Oreochromis niloticus, SDS-PAGE, DNA fragmentationAbstract
Pollution in aquatic ecosystems by various pollutants has been a concern in recent decades because of the devastating effect posed on the stability between the receiving water bodies and the biodiversity therein. Indigofera tinctoria has been used in a variety of fields, including health and medicine, as well as industrial applications such as textiles. The impacts of I. tinctoria on freshwater fishes, as well as the mechanisms underlying the biological effects, are, however, sparse. The goal of the study was to see how I. tinctoria affected Nile Tilapia (O. niloticus) juveniles' oxidative stress response, biochemical indicators, DNA disintegration, and protein profile. For 21 days, 6 varying concentrations were applied to the fish, including a control. The biochemical parameters in the test fish revealed a considerable variation in a dose-response manner. In I. tinctoria treated fish, the enzyme activity, when compared with control treatment, lipid peroxidation, DNA fragmentation, and oxidative stress all increased relative to the toxicant doses used. The protein profiling revealed a modification in the protein credentials in I. tinctoria-treated fish when compared to the control. As per the study, I. tinctoria is toxic to O. niloticus, particularly at higher doses. The results of this research could be applied to biomonitoring studies of I. tinctorial toxicity, particularly in industrial applications.
HIGHLIGHTS
- Pollution in aquatic ecosystems is a concern due to its impact on biodiversity
- Indigofera tinctoria, locally used as fabric dye was studied for its effects on Nile Tilapia juveniles
- Study finds I. tinctoria toxic, causing oxidative stress and DNA fragmentation, potentially affecting niloticus' muscle protein expression
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JL Gray, T Borch, ET Furlong, JG Davis, TJ Yager, YY Yang and DW Kolpin. Rainfall-runoff of anthropogenic waste indicators from agricultural fields applied with municipal biosolids. Sci. Total Environ. 2017; 580, 83-9.
EO Ayotunde, OA Fagbenro and OT Adebayo. Toxicity of aqueous extract of Moringa oleifera seed powder to Nile tilapia Oreochromis niloticus (LINNE I779), fingerlings. Int. Res. J. Agr. Sci. Soil Sci. 2011; 1, 142-50.
AF Samuel, V Mohan and LJ Rebecca. Physicochemical and heavy metal analysis of sugar mill effluent. J. Chem. Pharm. Res. 2014; 6, 585-7.
M Javed and N Usmani. Accumulation of heavy metals and human health risk assessment via the consumption of freshwater fish Mastacembelus armatus inhabiting, thermal power plant effluent loaded canal. SpringerPlus 2016; 5, 776.
C Zhao, S Yang, J Liu, C Liu, F Hao, Z Wang, H Zhang, J Song, SM Mitrovic and RP Lim. Linking fishes’ tolerance to water quality criteria for the assessment of environmental flows: A practical method for streamflow regulation and pollution control. Water Res. 2018; 141, 96-108.
IE Ekpo, MA Essien-Ibok and OI Obot. Assessing the environmental impact of aquatic macrophyte species structure in a near coastal tropical River, South Eastern Nigeria. J. Aquat. Sci. 2014; 29, 317-28.
JO Alagbe, JO Alagbe and TD Tijani. Effects of dried Centella Asiatica leaf meal as an herbal feed additive on the growth performance, haematology and serum biochemistry of broiler chicken. Pac. Int. J. 2018; 1, 172-80.
K Fern. Useful tropical plants database, Available at: http://tropical.theferns.info/viewtropical.php ?id=Shorea%20 stenoptera, accessed November 2021.
RHMJ Lemmens and D Cardon. Indigofera arrecta. In: PCME Jans and D Cardon (Eds.). Ressources végétales de l’Afrique tropicale, colorants et tannins: 100 - 105 (in French). Vol III. Fondation PROTA, Backhuys, CTA, Wageningen, The Netherlands, 2005.
AA Okoya and D Diisu. Adsorption of indigo-dye from textile wastewater onto activated carbon prepared from sawdust and periwinkle shell. Trends Appl. Sci. Res. 2021; 16, 1-9.
M Javed, N Usmani, I Ahmad and M Ahmad. Studies on the oxidative stress and gill histopathology in Channa punctatus of the canal receiving heavy metal-loaded effluent of Kasimpur Thermal Power Plant. Environ. Monit. Assess. 2015; 187, 4179.
I Ahmad and M Ahmad. Dacarbazine as a minor groove binder of DNA: Spectroscopic, biophysical and molecular docking studies. Int. J. Biol. Macromol. 2015; 79, 193-200.
HA Soliman, M Hamed, JS Lee and AEDH Sayed. Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate-induced oxidative stress and DNA damage in Clarias gariepinus. Environ. Pollut. 2019; 247, 678-84.
CE Grue, PL Gibert and ME Seeley. Neurophysiological and behavioral changes in non-target wildlife exposed to organophosphate and carbamate pesticides: Thermoregulation, food consumption, and reproduction. Am. Zool. 1997; 37, 369-88.
A Trang and PB Khandhar. Physiology, acetylcholinesterase. StatPearls Publishing, Florida, 2021.
S Bretaud, JP Toutant and P Saglio. Effects of carbofuran, diuron, and nicosulfuron on acetylcholinesterase activity in goldfish (Carassius auratus). Ecotoxicol. Environ. Saf. 2000; 47, 117-24.
A Karami, N Romano, T Galloway and H Hamzah. Virgin microplastics cause toxicity and modulate the impacts of phenanthrene on biomarker responses in African catfish (Clarias gariepinus). Environ. Res. 2016; 151, 58-70.
OI Muhammad, UM Mahmoud, F Fazio and AEDH Sayed. SDS-PAGE technique as a biomarker for fish toxicological studies. Toxicol. Rep. 2018; 5, 905-9.
G Frenzilli, M Nigro and BP Lyons. The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutat. Res. 2009; 681, 80-92.
CG Avio, S Gorbi, M Milan, M Benedetti, D Fattorini, G d’Errico, M Pauletto, L Bargelloni and F Regoli. Pollutants bioavailability and toxicological risk from microplastics to marine mussels. Environ. Pollut. 2015; 198, 211-22.
M Revel, F Lagarde, H Perrein-Ettajani, M Bruneau, F Akcha, R Sussarellu, J Rouxel, K Costil, P Decottignies, B Cognie, A Châtel, C Mouneyrac and C Mouneyrac. Tissue-specific biomarker responses in the blue mussel Mytilus spp. exposed to a mixture of microplastics at environmentally relevant concentrations. Front. Environ. Sci. 2019; 7, 33.
F Ribeiro, AR Garcia, BP Pereira, M Fonseca, NC Mestre, TG Fonseca, LM Ilharco and MJ Bebianno. Microplastics effects in Scrobicularia plana. Mar. Pollut. Bull. 2017; 122, 379-91.
AA Berber. Genotoxic evaluation of polystyrene microplastic. Sakarya Univ. J. Sci. 2019; 23, 358-67.
D Bussolaro, SL Wright, S Schnell, K Schirmer, NR Bury and VM Arlt. Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss). Environ. Pollut. 2019; 248, 706-14.
HM Burkill. The useful plants of West Tropical Africa. 2nd eds. Vol II. Royal Botanic Gardens, Richmond, 1985, p. 19-21.
DM Broom. Animal welfare: Concepts and measurement. J. Anim. Sci. 1991; 69, 4167-75.
RFD Maestro and W McDonald. Oxidative enzymes in tissue homogenates. In: RA Greenwald (Ed.). CRC handbook of methods for oxygen radical research. CRC Press, Florida, 2018, p. 291-6.
H Lück. Catalase. In: HU Bergmeyer (Ed.). Methods of enzymatic analysis. Academic Press, Massachusetts, 1965, p. 885-94.
MS Moron, JW Depierre and B Mannervik. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim. Biophys. Acta 1979; 582, 67-78.
DE Paglia and WN Valentine. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967; 70, 158-69.
GL Ellman, KD Courtney, V Andres Jr and RM Feather-stone. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 1961; 7, 88-95.
HU Bergmeyer, GN Bowes Jr, M Hörder and DW Moss. Provisional recommendations on IFCC methods for the measurement of catalytic concentrations of enzymes Part 2. IFCC method for aspartate aminotransferase. Clin. Chim. Acta 1976; 70, F19-F42.
H Ohkawa, N Ohishi and K Yagi. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979; 95, 351-8.
OM Popoola and FE Udoh. Preliminary study on genetic variation of Sudanonautes africanus in South-West, Nigeria using SDS-PAGE technique. Niger. J. Fish. 2020; 17, 1868-75.
D Boraschi and G Maurizi. Quantitation of DNA fragmentation with diphenylamine. In: A Cossarizza and D Boraschi (Eds.). Apoptosis-a laboratory manual of experimental methods. Purdue University Cytometry Laboratories, West Lafayette, India, 1998, p. 153-61.
P Spaak and M Bauchrowitz. Environmental influences and plankton dynamics. Eawag
News 2010; 69, 25-7.
DH Ogbuagu, ET Adebayo, AA Ayoade, OB Ugwu and DO Mba. Lead
accumulation in and its haematological effects on African catfish Clarias gariepinus. Afr. J. Aquat. Sci. 2015; 40, 201-4.
TD Katzenberger and KL Thorpe. Assessing the impact of exposure to microplastics in fish: Evidence Report - SC120056. Environment Agency, Bristol, 2015.
SE Hook, EP Gallagher and GE Batley. The role of biomarkers in the assessment of aquatic ecosystem health. Integr. Environ. Assess. Manag. 2014; 10, 327-41.
VI Lushchak, LP Lushchak, AA Mota and M Hermes-Lima. Oxidative stress and antioxidant defenses in goldfish Carassius auratus during anoxia and reoxygenation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2001; 280, R100-R107.
J Zhang, H Shen, X Wang, J Wu and Y Xue. Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 2004; 55, 167-74.
I Moreno, S Pichardo, A Jos, L Gómez-Amores, A Mate, CM Vazquez and AM Camean. Antioxidant enzyme activity and lipid peroxidation in the liver and kidney of rats exposed to microcystin-LR administered intraperitoneally. Toxicon 2005; 45, 395-402.
CM Ozoagudike and KA Bawa-Allah. Comparative assessment of the acute and sub-acute effects of some plant-based Ichthyotoxins on aquatic organisms: A case study of Clarias gariepinus. Proc. Niger. Acad. Sci. 2020; 13, 35-45.
FO Mowuogwu and BO George. Comparative antioxidant activity of hibiscus sabdariffa and ascorbic acid on ferrous sulphate-induced oxidative stress in Clarias gariepinus. East Cent. Afr. J. Pharm. Sci. 2008; 11, 39-42.
AM EL-Gazzar, KE Ashry and YS El-Sayed. Physiological and oxidative stress biomarkers in the freshwater Nile tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. Alex. J. Vet. Sci. 2014; 40, 29-43.
S Guilherme, I Gaivão, MA Santos and M PAChEco. DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide-elucidation of organ-specificity and the role of oxidative stress. Mutat. Res. 2012; 743, 1-9.
UEA Warraich, F Hussain and HUR Kayani. Aging-oxidative stress, antioxidants and computational modeling. Heliyon 2020; 6, e04107.
VI Lushchak, TV Bagnyukova, VV Husak, LI Luzhna, OV Lushchak and KB Storey. Hyperoxia results in transient oxidative stress and an adaptive response by antioxidant enzymes in goldfish tissues. Int. J. Biochem. Cell Biol. 2005; 37, 1670-80.
M Hasanuzzaman, K Nahar, TI Anee and M Fujita. Glutathione in plants: Biosynthesis and physiological role in environmental stress tolerance. Phys. Mol. Biol. Plants 2017; 23, 249-68.
A Gehin, C Guyon and L Nicod. Glyphosate-induced antioxidant imbalance in HaCaT: The protective effect of Vitamins C and E. Environ. Toxicol. Pharmacol. 2006; 22, 27-34.
PH Dussault, AD George and TK Trullinger. Peroxides as oxidative enzyme inhibitors: Mechanism-based inhibition of a cysteine protease by an amino acid ozonide. Bioorg. Med. Chem. Lett. 1999; 9, 3255-8.
CD Nwani, NS Nagpure, R Kumar, B Kushwaha and WS Lakra. DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environ. Toxicol. Pharmacol. 2013; 36, 539-47.
CD Nwani, CT Ifo, HO Nwamba, VC Ejere, GC Onyishi, SN Oluah, SN Oluah, OE Ikwuagwu and GE Odo. Oxidative stress and biochemical responses in the tissues of African catfish Clarias gariepinus juvenile following exposure to primextra herbicide. Drug Chem. Toxicol. 2015; 38, 278-85.
PS Paruruckumani, A Maharajan, V Ganapiriya, Y Narayanaswamy and RR Jeyasekar. Surface ultrastructural changes in the gill and liver tissue of Asian sea bass Lates calcarifer (Bloch) exposed to copper. Biol. Trace Elem. Res. 2015; 168, 500-7.
DSP Patterson. Structure, metabolism and toxicity of aflatoxins: A review. Cahiers de Nutrit et de dietet 1976; 11, 71-6.
K Sharaf-Eldeen and NA Abdel-Hamid. Sublethal effects of copper sulfate, malathion and paraquat on protein pattern of Oreochromis niloticus. Egypt. J. Aquat. Biol. Fish. 2002; 6, 167-82.
G Tripathi and SP Shukla. Malate and lactate dehydrogenases of a freshwater catfish: Impact of endosulfan. Biomed. Environ. Sci. 1990; 3, 52-64.
EA Badaway and SS El-Serafy. Comparative biochemical genetic studies on Clarias gariepinus from different polluted localities. Menoufya J. Agric. Res. 1998; 23, 1705-15.
OM Popoola, OR Balogun and MA Oyelade. Toxicological effect of N-(phosphonomethyl) glycine (Glyphosate) on protein expression and oxidative stress biomarker in African catfish (Clarias gariepinus, Burchell 1822) juvenile. Unios. J. Agr. Renew. Resour. 2021; 5, 55-67.
A Manke, L Wang and Y Rojanasakul. Mechanisms of nanoparticle-induced oxidative stress and toxicity. BioMed Res. Int. 2013; 2013, 942916.
H Cui, Y Kong and H Zhang. Oxidative stress, mitochondrial dysfunction, and aging. J. Signal Transduct. 2012; 2012, 646354.
K Srikanth, E Pereira, AC Duarte, I Ahmad and JV Rao. Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells. Environ. Sci. Pollut. Res. Int. 2015; 22, 15571-8.
OM Popoola. Toxicity effect of atrazine on histology, haematology, and biochemical indices of Clarias gariepinus. Int. J. Fish. Aquat. Stud. 2018; 6, 87-92.
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