Cytotoxicity Evaluation of Hydrogel Sheet Dressings Fabricated by Gamma Irradiation: Extract and Semi-Direct Contact Tests
DOI:
https://doi.org/10.48048/tis.2022.4583Keywords:
Hydrogel sheet dressings, Gamma irradiation, MTT assay, Extract test, Semi-direct contact testAbstract
Cytotoxicity presents one of the required criteria in the biological evaluation of medical devices. In this study, the semi-direct contact test was used to evaluate the potential cytotoxicity of hydrogel wound dressings compared to the conventional extract test. Three types of hydrogel sheets were fabricated from poly(vinyl alcohol) (PVA) by gamma irradiation: Bare sheets, silver (Ag)-coated sheets, and Aloe vera (AV)-coated sheets. In the extract test, L929 cells were cultured in the extract derived from the elution of hydrogel samples. For the semi-direct contact test, the cells were cultured in situ with the hydrogel samples placed inside transwell inserts above the cell monolayers. At the endpoint of both tests, MTT assay was performed, and the cell viability was determined from the absorbance of formazan. Only the bare and AV-coated hydrogel sheets showed cell viability above the 70 % threshold that ensured the non-cytotoxicity by both tests. For Ag-coated sheets, less than 70 % cell viability occurred when Ag coating was ³ 0.2 mg/cm2. Interestingly, the formazan-depleted area underneath the Ag-coated sample could be clearly observed by the semi-direct contact test. The release of Ag in the form of nanoparticles was confirmed by UV-Vis absorption at 420 nm. In conclusion, the semi-direct contact test can serve as a reliable alternative to the conventional extract test in evaluating the potential cytotoxicity of hydrogel wound dressings.
HIGHLIGHTS
- A semi-direct contact test with transwell inserts was investigated as an alternative approach to the conventional extract test in evaluating the cytotoxicity of hydrogel wound dressings
- Leachable substances from the hydrogel dressings permeated through the porous membrane of transwell inserts to the reach the cell monolayer underneath
- Cell viability results based on MTT assay obtained from semi-direct contact test were in agreement with the extract test
- Only the semi-direct contact test provided the visual assessment of localized toxicity caused by the release of silver nanoparticles from hydrogel sheets onto the cell monolayer
GRAPHICAL ABSTRACT
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