Assessment of Bacterial Community Profile in the Rearing Pond Environment and the Intestinal Tract of Pacific White Shrimp Litopenaeus vannamei in Lampung Province, Indonesia using 16S rRNA Gene Amplicon Sequencing: A Short Research Investigation
DOI:
https://doi.org/10.48048/tis.2023.3418Keywords:
Aquaculture environment, Bacterial diversity, Intestinal tract, Metagenomic, Whiteleg shrimpAbstract
Along with the high demand for shrimp, the production challenges faced by practitioners are increasing in Indonesia, one of which is in Lampung region. Various basic monitoring techniques regarding the environment and farmed shrimp are needed to control production sustainability. This present study aimed to identify the bacterial community profile in the rearing pond water and intestinal tract of Pacific white shrimp Litopenaeus vannamei. Illumina-based sequencing was chosen to determine the bacterial community using the V3-V4 region of the bacterial 16S rRNA gene. Sequence data revealed the differences in bacterial community structure between the rearing water and shrimp intestines. Proteobacteria was the most prevalent phylum in the rearing water (W.B), accounting for 45.29 %, followed by Cyanobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria. In the shrimp intestinal tract (S.B), Cyanobacteria (35.15 %) dominated the microbiota, followed by Proteobacteria, Saccharibacteria, Actinobacteria, Bacteroidetes, Verrucomicrobia, TM6 (Dependentiae), and Firmicutes. Cyanobacteria were higher in the shrimp intestines (35.15 %) than in the rearing water (26.63 %). In addition, Escherichia-Shigella was the most common genera in the rearing water and shrimp intestines with different relative abundance. Cyanobacteria and Escherichia-Shigella highly detected in the rearing water and shrimp’s intestines might indicate that pond water had been polluted. Further investigation is necessary on the correlation of Cyanobacteria in the shrimp intestines with water pollution, proven by the dominance of Cyanobacteria and Escherichia-Shigella. These findings provide basic information to enhance our understanding of the microbial community and their roles in the shrimp culture environment to improve the quantity and quality of the yield and support its sustainability.
HIGHLIGHTS
- Bacterial community profile in the rearing water and the intestinal tract of Pacific whiteleg shrimp play important roles in shrimp production
- Bacterial community structure differed between the rearing water and shrimp intestines
- Proteobacteria was the most abundant phylum in the rearing water (W.B), while Cyanobacteria dominated the microbiota in the intestinal tract of Pacific whiteleg shrimp
- Cyanobacteria and Escherichia-Shigella highly detected in the rearing water and shrimp’s intestines might indicate that pond water had been polluted
GRAPHICAL ABSTRACT 
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