Evaluation of Antibacterial Activity of Salvia stepposa Extracts Isolated using Microwave Extraction, Growing Wild in Kazakhstan
DOI:
https://doi.org/10.48048/tis.2022.3217Keywords:
Steppe sage, Extract, Antibacterial activity, Microwave extraction, Disk diffusion methodAbstract
The studies on antibacterial activity of 16 samples of Salvia stepposa (steppe sage) ethanolic extracts, an indigenous plant of Central Kazakhstan, against Staphylococcus aureus (ATCC 6538), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231) were assayed. Leaves and flowers of Salvia stepposa were extracted by ethanol 30, 40, 70 and 90 % using conventional and microwave extraction. Antibacterial screening performed by disc-diffusion method was conducted, the concentration of all extracts was 100 mg/mL. The results revealed that the highest activity against Staphylococcus aureus and Bacillus subtilis have leaves and flowers extract of steppe sage in all solvents except 70 % microwave leaf extract. The only extract that showed antibacterial activity against all strains of microorganisms used is 40 % microwave leaf extract of steppe sage with growth inhibition zones for Staphylococcus aureus 23.5 0.65 mm, for Bacillus subtilis 38 1.3 mm, for Escherichia coli 16.5 1.72 mm, for Candida albicans 16 2.6 mm, respectively. Was found that 70 % of leaf extract of the steppe sage obtained under microwave irradiation has the lowest activity. The MIC values for tested extracts were evaluated too.
HIGHLIGHTS
- Salvia stepposa is a widespread plant on the territory of Kazakhstan the properties of which still remain unexplored
- Antibacterial activity and MIC of 16 examples obtained different methods was evaluated
- MAE is an interesting alternative to CE of extracting plant raw materials, which can reduce extraction time and energy consumption
Downloads
References
M Cioch, P Satora, M Skotniczny D Semik-Szczurak and T Tarko. Characterisation of antimicrobial properties of extracts of selected medicinal plants. Pol. J. Microbiol. 2017; 66, 463-72.
S Nabavi, A di Lorenzo, M Izadi, E Sobarzo-Sánchez, M Daglia and SM Nabavi. Antibacterial effects of cinnamon: From farm to food, cosmetic and pharmaceutical industries. Nutrients 2015; 7, 7729-48.
CSC Garcia, C Menti, APF Lambert, T Barcellos, S Moura, C Calloni, CS Branco, M Salvador, M Roesch-Ely and JAP Henriques. Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): Antioxidant, and antitumor in mammalian cells. An. Acad. Bras. Ciências 2016; 88, 281-92.
RM Harfouch, M Darwish, W Al-Asadi, AF Mohammad, NM Gharib and M Haroun. Antibacterial activity of essential oils of Rosmarinus officinalis, Salvia officinalis and Anthemis nobilis widespread in the Syrian coast. Res. J. Pharm. Tech. 2019; 12, 3410-2.
K Gayathiri, JGS Kumar and S Kavimani. Evaluation of immunomodulatory activity of Salvia hispanica. L seeds. Res. J. Pharm. Tech. 2017; 10, 4255-60.
Vidyasabbani, A Ramesh, Snehalatha, B Rahul, Sriharitha, Sanjayvarma and Aparna. In-vitro and in-vivo anti-inflammatory activities of Salvia hispanica and Linum usitatissium seeds in Swiss Albino rats. Res. J. Pharm. Tech. 2015; 8, 1438-44.
R Sawant, A Baghkar, S Jagtap, L Harad, A Chavan, NA Khan, RP Yevale and MK Kale. A review on - herbs in anticancer. Asian J. Res. Pharm. Sci. 2018; 8, 179-84.
N Muthukumar and NS Veerappa. Powder microscopic analysis on leaf, petiole, stem and root on Thespesia populnea (L.) Soland ex Correa. Res. J. Pharmacognosy Phytochem. 2019; 11, 189-92.
A Nayak, RR Rao, PJ Shenoy, H Sindhu, S Teerthanath and Bhuvaneshwari. Salvia hispanica (Chia) seeds afford hepatoprotection against isoniazid and rifampicin induced toxicity in a murine model. Res. J. Pharm. Tech. 2020; 13, 4805-10.
M Acimovic, B Kiprovski, M Rat and MD Brdar-Jokanovic. Salvia sclarea: Chemical composition and biological activity. J. Agron. Tech. Eng. Manag. 2018; 1, 18-28.
I Sengun, E Yucel, G Kilic and B Ozturk. Antimicrobial activities of Salvia sclarea L. and Salvia aethiopis L. essential oils. In: Proceedings of the International Conference on Agronomy and Food Science & Technology, Istanbul, Turkey. 2019.
H Cui, X Zhang, H Zhou, C Zhao and L Lin. Antimicrobial activity and mechanisms of Salvia sclarea essential oil. Bot Stud. 2015; 56, 16.
The state pharmacopoeia of the Republic of Kazakhstan. Vol III. Zhibek zholy, Almaty, Kazakhstan, 2014, p. 801.
Ya Levaya, G Atazhanova and M Ishmuratova. Macroscopic analysis of Salvia stepposa. In: Proceedings of the 8th International Scientific and Practical Conference, Poltava, Ukraine. 2020, p. 174-7.
B Tyagi, A Dubey, AK Verma and S Tiwari. Antibacterial activity of phenolics compounds against pathogenic bacteria. Int. J. Pharm. Sci. Rev. Res. 2015; 35, 16-8.
L Bouarab-Chibane, V Forquet, P Lantéri, Y Clément, L Léonard-Akkari, N Oulahal, P Degraeve and C Bordes. Antibacterial properties of polyphenols: Characterization and QSAR (quantitative structure - activity relationship). Front. Microbiol. 2019; 10 ,829.
PJ Desai and P Parikh, Extraction of natural products using microwaves as a heat source. Sep. Purif. Rev. 2010; 39, 1-32.
P Tatke and Y Jaiswal. An overview of microwave assisted extraction and its applications in herbal drug research. Res. J. Med. Plant. 2011; 5, 21-31.
DJ Alderman and P Smith. Development of draft protocols of standard reference methods for antimicrobial agent susceptibility testing of bacteria associated with fish diseases. Aquaculture 2001; 232, 511-24.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
