Spasmolytic Effect of Papaya (Carica papaya L.) Leave Alkaloid on Isolated Rat Myometrial Contraction in vitro
DOI:
https://doi.org/10.48048/tis.2022.2684Keywords:
Carica papaya, Papaya alkaloids, Uterine contraction, Uterine relaxationAbstract
This study aimed to investigate the action of crude alkaloid extract from papaya leaves (PA) on the isolated rat myometrium in vitro. The alkaloids were extracted from papaya leaves using conventional acid-base method. The myometrium strips were taken from female Wistar rats pretreated with diethylstilbestrol (100 mg) for 24 h before the experiment. A preliminary experiment has shown that PA had no effect on myometrial contraction. However, PA (10-6 - 10-3 gm/mL) significantly inhibited myometrium contraction pre-contracted with a depolarizing solution in a concentration-dependent fashion. PA (10-5, 3×10-5 or 10-4 gm/mL) or verapamil (10-9, 10-8 and 10-7 M) were able to inhibit the myometrial contractile response to CaCl2 (10-5 - 3×10-2 M) in Ca2+-free solution and cause a rightward shift of log concentration-response curve. PA also significantly inhibited myometrium contraction produced by oxytocin (1 mU/mL), ACh (3×10-5 M) and PGF2a (10-5 M). Furthermore, propranolol (10-7 M) caused no effect on uterine relaxation induced by PA (10-6 - 10-3 gm/mL), although it inhibited uterine relaxation induced by isoproterenol (10-10 - 10-5 M). These results indicated that PA was a uterine relaxant. Its mechanism of action may be unrelated to stimulation of b2-adrenergic receptors of the uterus. This action may be contributed to mainly the Ca2+ influx, possibly through voltage-operated Ca2+ channel on the plasma membrane, and to a lesser extent by a rise in Ca2+ release from internal storage. However, the action of PA at intracellular sites, downstream of Ca2+ influx such as the contractile machinery of the myometrium, cannot be excluded.
HIGHLIGHTS
- Alkaloid extract from Carica papayaleaves has shown to be a uterine relaxant on isolated rat uterus in vitro
- The alkaloid extract was able to inhibit uterine contraction stimulated by various uterine stimulants such as acetylcholine, oxytocinand prostaglandin F2alpha
- The inhibition on uterine contraction may be due to the inhibition on calcium influx into the uterine muscle cells
GRAPHICAL ABSTRACT
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