Exposure of Copper Nano-Particles (CuNPs) Impairs Developmental Stages and Hatchability in the Fresh Water Snail Indoplanorbis exustus (Planorbidae: Pulmonate)
DOI:
https://doi.org/10.48048/tis.2022.2674Keywords:
Copper nanoparticles, Veliger, genotoxic, Hatchability, Survival, Developmental stressAbstract
Pollution of water bodies by humans, in our day-to-day activities impairs the quality of water, thereby making the water sources unsuitable for aquatic fauna and also for human health. Various sources of pollutants in excess may negatively affect the diversity of living organisms. The evolving growth of nanotechnology has attracted a great deal of attention due to its concern in assessing their environmental and health safety. The present study was conducted to investigate toxicity of copper nanoparticles (CuNP) on the development of fresh water snail Indoplanorbis exustus. Acclimatized snails were exposed to increasing concentration of CuNP for 96 h and acute toxicity experiment was carried out to determine LC50, followed by exposure of snails to sublethal concentrations of CuNP to observe developmental deformities and to estimate percentage hatchability and survivability of young ones. The projected LC50 value for 96h of CuNPs was found to be 5.8mg/L, followed by exposure to sub-lethal concentrations of 1/6th(0.96mg/L), 1/5th(1.16mg/L), 1/4th(1.45mg/L), and 1/3th (1.93mg/L). The highest concentration of (1.93mg/L;1/3thof LC50) retarded hatching and caused morphological deformities in the larvae. However, no significant difference was observed in the weight of embryos in 7 days. These results revealed that CuNP exposure at sub lethal concentrations exerted developmental stress.
HIGHLIGHTS
- Most of the invertebrate models used in aquatic toxicity are from phylum Mollusca, the gastropods are considered as the best model organisms for NPs toxicity because they are adapted to various environmental disturbances due to their dual habitat, easy availability, high fecundity, small size, short generation time and adaptability.
- Snails, Indoplanorbis exustus were exposed to increasing concentration of copper nanoparticles (CuNPs) for 96 h and acute toxicity experiment was carried out to determine LC50, followed by exposure of snails to sublethal concentrations of CuNPs to observe developmental deformities and to estimate percentage hatchability and survivability of young ones
- The highest concentration of (1.93 mg/L; 1/3th of LC50) retarded hatching and caused morphological deformities in the larvae. However, no significant difference was observed in the weight of embryos in 7 days. These results revealed that CuNP exposure at sub lethal concentrations exerted developmental stress on snails
Downloads
References
G Borkow and J Gabbay. Copper, an ancient remedy returning to fight microbial, fungal and viral infections. Curr. Chem. Biol. 2009; 3, 272-8.
AA Bazrafshan, M Ghaedi, S Hajati, R Naghiha and A Asfaram. Synthesis of ZnO-nanorod-based materials for antibacterial, antifungal activities, DNA cleavage and efficient ultrasound-assisted dyes adsorption. Ecotoxicol. Environ. Saf. 2017; 142, 330-7.
I Corsi, M Winther-Nielsen, R Sethi, C Punta, CD Torre, G Libralato, G Lofrano, L Sabatini, M Aiello, L Fiordi, F Cinuzzi, A Caneschi, D Pellegrini and I Buttino. Ecofriendly nanotechnologies and nanomaterials for environmental applications: Key issue and consensus recommendations for sustainable and ecosafenano remediation. Ecotoxicol. Environ. Saf. 2018; 154, 237-44.
T Gomes, JP Pinheiro, I Cancio, CG Pereira, C Cardosoand MJ Bebianno. Effects of copper nanoparticles exposure in the Mussel Mytilusgalloprovincialis. Environ. Sci. Technol. 2011, 45, 9356-62.
S Chaturvedi and PN Dave. Nanomaterials: Environmental human health risk. In: CM Hussain (Ed.). Handbook of nanomaterials for industrial applications. Elsevier, Amsterdam, 2018, p. 1055-62.
B Fadeel, L Farcal, BA Hardy, S Azquez-Campos, D Hristozov, A Marcomini, I Lynch, E Valsami-Jones, H Alenius and K Savolainen. Advanced tools for the safety assessment of nanomaterials. Nat. Nanotechnol. 2018; 13, 537-43.
R Varshney, S Bhadauria and MS Gaur. A review: Biological synthesis of silver and copper nanoparticles. Nano Biomed. Eng. 2012; 2012, 4.
F Gilavand, R Saki, S Mirzaei, H Lashgarian, M Karkhane and A Marzban. Green synthesis of zinc nanoparticles using aqueous extract of Magnoliaeofficinalis and assessment of its bioactivity potentials. Biointerface Res. Appl. Chem. 2020; 2020, 11.
M Gondwal and GJN Pant.Synthesis and catalytic and biological activities of silver and copper nanoparticles using Cassia occidentalis. Int. J. Biomate. 2018; 10, 1-10.
S Bansod and M Rai. Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and A. niger. World J. Medical Sci. 2008; 3, 81-8.
EC Oliveira-Filho, E Nakano and L Tallarico. Bioassays with freshwater snails Biomphalariasp.: From control of hosts in public health to alternative tools in ecotoxicology. Invertebr. Reprod. Dev. 2017; 78, 983-1002.
A Salmi, R Rouabhi, N Toualbia, C Taib, H Chenikher, S Gasmi and S Boussekine. Subcellular study of the cadmium selenide nanoparticles (CdSe-NPs) toxicity on a terrestrial bioindicator model Helix aspersa. Toxicol. Environ. Health Sci. 2017; 9, 135-40.
J Amorim, I Abreu, P Rodrigues, D Peixoto, C Pinheiro, A Saraiva, AP Carvalho, L Guimaraes, LO Teles. Lymnaeastagnalis as a freshwater model invertebrate for ecotoxicological studies. Sci. Total. Environ. 2019; 699, 11-28.
M Kaloyianni, A Dimitriadi, M Ovezik, D Stamkopoulou, K Feidantsis, G Kastrinaki, G Gallios, I Tsiaoussis, G Koumoundouros and D Bobori. Magnetite nanoparticles effects on adverse responses of aquatic and terrestrial animal models. J. Hazard Mater. 2020; 383, 121204.
BS Khangarot and S Das. Effects of copper on the egg development and hatching of a freshwater pulmonate snail Lymnaealuteola L. J. Hazard Mater. 2010; 179, 665-75.
Z Pirger, Z Zrinyi, G Maasz, E Molnar and T Kiss. Pond snail reproduction as model in the environmental risk assessment: Reality and doubts. In: S Ray (Ed.). Biological resources of water. Intech Open, London, 2018.
M Schirling, A Bohlen, R Triebskorn and HR Kohler. An invertebrate embryo test with the apple snail Marisa cornuarietis to assess effects of potential developmental and endocrine disruptors. Chemosphere 2006; 64, 1730-8.
PS Araújo, MC Caixeta, RS Brito, BB Gonçalves, SM Silva, ECO Lima, LD Silva, JC Bezerra and TL Rocha. Molluscicidal activity of polyvinylpyrrolidone (PVP)-functionalized silver nanoparticles to Biomphalariaglabrata: Implications for control of intermediate host snail of schistosomiasis. Acta Tropica 2020; 211, 105644.
DJ Finney. Probit analysis. 3rd eds. 1971, p. 333.
R Jeyaraman, J Kadarkaraithangam, M Arumugam and R Govindasamy. Copper nanoparticles synthesized by polyol process used to control hematophagous parasites. Parasitol. Res. 2011; 109, 1403-15.
European Commission. Commission Recommendation of 18 October 2011 on the definition of nanomaterial, 2011/696/EU. Official Journal of the EU L275, European Commission, p. 38-40.
K Ruppert, C Geib, C Askem, R Benstead, R Brown, M Coke, V Ducrot, P Egeler, H Holbech and TH Hutchinson. Development and validation of an OECD reproductive toxicity test guideline with the mud snail Potamopyrgusantipodarum (Mollusca, Gastropoda). Chemosphere 2017; 181, 589-99.
T Sarraude. Transgenerational endocrine disruption: Does elemental pollution affect egg or nestling thyroid hormone levels in a wild songbird? Environ. Pollut. 2019; 247, 725-35.
S Besnaci, S Bensoltane, F Moulka and H Braia. Embryotoxicity evaluation of iron oxide Fe2O3 on land snails: Helix aspersa. J. Entomol. Zool. Stud. 2016; 4, 317-23.
BSR Khangarot. Sensitivity of a freshwater pulmonate snails, Lymnaea luteola L., to heavy metals. Bull. Environ. Contam Toxicol. 1988; 41, 208-13.
ME Omobhude, OA Morenikeji and OT Oyeyemi. Molluscicidal activities of curcumin-nisin polylactic acid nanoparticle on Biomphalaria pfeifferi. PLoS Neglected Trop. Dis. 2017; 23, e0005855.
W Bai, W Tian, Z Zhang, X He, Y Ma, N Liu and Z Chai. Effects of copper nanoparticles on the development of zebrafish embryos. J. Nanosci. Nanotechnol. 2010; 10, 8670-6.
M Vasconcelos-Lima, MIA Pereira, PEC Filho, WN de Siqueira, HAMF Silva, EJ França, BS Santos, M de Albuquerque, AM Melo and A Fontes. Studies on toxicity of suspensions of CdTe quantum dots to Biomphalaria glabrata mollusks. Environ. Toxicol. Chem. 2019; 38, 2128-36.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
