Diversity of Colony Morphotypes, Biochemical Characteristics, and Drug Susceptibility Patterns of Burkholderia pseudomallei Isolated from Humans, Animals, and Environmental Sources in Thailand

Authors

  • Warinda Prommachote Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Wanida Mala Center of Excellence Research for Melioidosis and Microorganisms (CERMM), Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Jirarat Songsri Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Jaruwan Khoosuilee Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Sasitorn Wansu Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Jirasaya Srisara Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Jiraporn Kumkrue Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Pakitta Phanubol Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand
  • Wiyada Kwanhian Klangbud Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand https://orcid.org/0000-0002-5247-166X

DOI:

https://doi.org/10.48048/tis.2022.153

Keywords:

Biochemical test, Burkholderia pseudomallei, Ceftazidime, Colony morphology, Drug susceptibility pattern, Melioidosis, Minimum inhibitory concentration

Abstract

Melioidosis is an infection that occurs in humans and a wide range of animals by Burkholderia pseudomallei as a causative pathogen, which inhabits environments of tropical and subtropical climate zones, particularly in Thailand. The standard laboratory diagnosis is by culture and biochemical characteristic differentiation. This study aimed to determine the diversity of colony morphology, biochemical characteristics, and drug susceptibility patterns of a B. pseudomallei collection isolated from humans, animals, soil, and water in Thailand. According to the previous characterization of colony morphotypes from Type I to VII, Type I is predominant with B. pseudomallei from humans and animals with a similar water source. Meanwhile, Type III is predominant from soil sources. The results confirmed the hypothesis that infection might be from water instead of soil exposure. The typical biochemical phenotypes were consistent among different isolate sources, including cytochrome oxidase (positive), triple iron sugar (K/N or A/N), sulfide (negative), indole (negative), non-motile, glucose oxidizing, and DNase (positive). The biochemical characteristic results supported the data for the selection of the prepopulated biochemical tests in clinical and research laboratories. The antimicrobial susceptibility patterns by standard disk diffusion showed mode values of inhibition zones for selected drugs that had lower trends in B. pseudomallei isolated from the soil than other sources. The results were consistent with MIC50 and MIC90 values of ceftazidime, which was highest in B. pseudomallei from the soil than other sources.

HIGHLIGHTS

  • The previous study reported the characterization of Burkholderia pseudomallei colony morphotypes from Type I to VII. However, there were no reports of the diversity of colony morphology and antibiotic susceptibility pattern from diverse sources
  • Type I is predominant with B. pseudomallei from humans and animals with a similar water source, which confirmed the hypothesis that infection might be from water instead of soil exposure
  • The mode of antimicrobial inhibition zones for selected drugs had lower trends in the isolates from the soil than other sources. The results indicated the soil isolates showed a trend in increasing ceftazidime resistance compared with other sources


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References

S Hinjoy, V Hantrakun, S Kongyu, J Kaewrakmuk, T Wangrangsimakul, S Jitsuronk, W Saengchun, S Bhengsri, T Akarachotpong, S Thamthitiwat, O Sangwichian, S Anunnatsiri, RW Sermswan, G Lertmemongkolchai, CS Tharinjaroen, K Preechasuth, R Udpaun, P Chuensombut, N Waranyasirikul, C Anudit, S Narenpitak, Y Jutrakul, P Teparrukkul, N Teerawattanasook, K Thanvisej, A Suphan, P Sukbut, K Ploddi, P Sirichotirat, B Chiewchanyon, K Rukseree, M Hongsuwan, G Wongsuwan, P Sunthornsut, V Wuthiekanun, S Sachaphimukh, P Wannapinij, W Chierakul, C Chewapreecha, J Thaipadungpanit, N Chantratita, S Korbsrisate, A Taunyok, S Dunachie, P Palittapongarnpim, S Sirisinha, R Kitphati, S Iamsirithaworn, W Chaowagul, P Chetchotisak, T Whistler, S Wongratanacheewin and D Limmathurotsakul. Melioidosis in Thailand: Present and future. Trop. Med. Infect. Dis. 2018; 3, 38.

WJ Wiersinga, HS Virk, AG Torres, BJ Currie, SJ Peacock, DAB Dance and D Limmathurotsakul. Melioidosis. Nat. Rev. Dis. Primers 2018; 4, 17107.

W Kwanhian, T Jiranantasak, AT Kessler, BE Tolchinsky, S Parker, J Songsri, S Wisessombat, K Pukanha, VA Testamenti, P Khrongsee, S Sretrirutchai, J Kaewrakmuk, J Tangpong and A Tuanyok. Investigation of melioidosis outbreak in pig farms in southern Thailand. Vet. Sci. 2020; 7, 9.

V Hantrakun, J Thaipadungpanit, P Rongkard, P Srilohasin, P Amornchai, S Langla, M Mukaka, N Chantratita, V Wuthiekanun, DAB Dance, NPJ Day, SJ Peacock and D Limmathurotsakul. Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei. PLoS Negl. Trop. Dis. 2018; 12, e0006193.

N Chantratita, V Wuthiekanun, K Boonbumrung, R Tiyawisutsri, M Vesaratchavest, D Limmathurotsakul, W Chierakul, S Wongratanacheewin, S Pukritiyakamee, NJ White, NPJ Day and SJ Peacock. Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei. J. Bacteriol. 2007; 189, 807-17.

MT Kenfack, M Mazur, T Nualnoi, TL Shaffer, A Ngassimou, Y Blériot, J Marrot, R Marchetti, K Sintiprungrat, N Chantratita, A Silipo, A Molinaro, DP AuCoin, MN Burtnick, PJ Brett and C Gauthier. Deciphering minimal antigenic epitopes associated with Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharide O-antigens. Nature Commun. 2017; 8, 115.

S Mohanty, S Sarkar and B Mishra. Melioidosis of the head and neck: A case series from eastern India. Infect. Dis. Reports 2020; 12, 36-45.

BN Ross, JN Myers, LA Muruato, D Tapia and AG Torres. Evaluating new compounds to treat Burkholderia pseudomallei infections. Front. Cell Infect. Microbiol. 2018; 8, 210.

JV Bugrysheva, C Lascols, HP McLaughlin, JE Gee, MG Elrod and D Sue. Antimicrobial susceptibility of western hemisphere isolates of Burkholderia pseudomallei: Phenotypic and genomic analyses. Microb. Drug Resist. 2021; 27, 1176-85.

SHY Fen, S Tandhavanant, R Phunpang, P Ekchariyawat, N Saiprom, C Chewapreecha, R Seng, E Thiansukhon, C Morakot, N Sangsa, S Chayangsu, S Chuananont, K Tanwisaid, W Silakun, N Buasi, S Chaisuksant, T Hompleum, P Chetchotisakd, NPJ Day, W Chantratita, G Lertmemongkolchai, TE West and N Chantratita. Antibiotic susceptibility of clinical Burkholderia pseudomallei isolates in northeast Thailand during 2015-2018 and the genomic characterization of beta-lactam-resistant isolates. Antimicrob. Agents Chemother. 2021; 65, e02230.

P sakdinun, T Paethaisong, R Naksuwan and N Sriwongsa. Monitoring of Burkholderia pseudomallei in animals from slaughterhouses in Nakhon Pathom and Ratchaburi provinces. KKU Vet. J. 2018; 28, 35-43.

C Edler, H Derschum, M Köhler, H Neubauer, H Frickmann and RM Hagen. Comparison of mast Burkholderia cepacia, Ashdown + Gentamicin, and Burkholderia pseudomallei selective agar for the selective growth of Burkholderia spp. Eur. J. Microbiol. Immunol. 2017; 7, 15-36.

KC Carroll. Manual of clinical microbiology. 12th ed. ASM Press, Washington DC, 2019.

J Songsri, Y Kinoshita, W Kwanhian, S Wisessombat, J Tangpong, MS Rahman-Khan and A Tuanyok. Cross-reactivity of latex agglutination assay complicates the identification of Burkholderia pseudomallei from soil. FEMS Microbiol. Lett. 2018; 365, fny256.

R Martzy, K Bica-Schröder, ÁM Pálvölgyi, C Kolm, S Jakwerth, AKT Kirschner, R Sommer, R Krska, RL Mach, AH Farnleitner and GH Reischer. Simple lysis of bacterial cells for DNA-based diagnostics using hydrophilic ionic liquids. Sci. Rep. 2019; 9, 13994.

Y Lin, Q Wu, X Liu, S Dong, L Wu, H Pei, K Xu and Q Xia. Molecular tracking investigation of melioidosis cases reveals regional endemicity in Hainan, China. Biomed. Rep. 2016; 5, 766-70.

MSM Nassar, WA Hazzah and WMK Bakr. Evaluation of antibiotic susceptibility test results: How guilty a laboratory could be? J. Egypt. Public Health Assoc. 2019; 94, 4.

Wayne. Performance standards for antimicrobial susceptibility testing M100-S25. In: Proceedings of the 25th Informational Supplement. Clinical and Laboratory Standards Institute, 2020.

CLSI. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. 3rd ed. CSLI document M45-A2. PA, Clinical and Laboratory Standards Institute, 2015.

P Panomke, P Wongsana, S Wanram and S Wongratanacheewin. Burkholderia pseudomallei biofilm plays a key role in chronic inflammation in C57BL/6 mice. Southeast Asian J. Trop. Med. Public Health 2017; 48, 73-82.

The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0, 2021, Available at: http://www.eucast.org, accessed May 2021.

DAB Dance, V Wuthiekanun, RW Baird, R Norton, D Limmathurotsakul and BJ Currie. Interpreting Burkholderia pseudomallei disc diffusion susceptibility test results by the EUCAST method. Clin. Microbiol. Infect. 2021; 27, 827-9.

S Dutta, S Haq, MR Hasan and JA Haq. Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh. BMC Res. Notes 2017; 10, 299.

DR Lonsway, MG Elrod, N Kendrick, R Tiller, MM Sullivan, JR Edwards, DD Blaney and M Karlsson. Correlation between Etest and reference broth microdilution for antimicrobial susceptibility testing of Burkholderia pseudomallei. Microbial Drug Resist. 2019; 26, 311-8.

MA Sadiq, L Hassan, SA Aziz, Z Zakaria, HI Musa and MM Amin. The relationship between bacterial sources and genotype to the antimicrobial resistance pattern of Burkholderia pseudomallei. Vet. World 2018; 11, 1404-8.

S Smith and J Hanson. Successful treatment of localized cutaneous melioidosis with oral antibiotic therapy alone. American J. Trop. Med. Hyg. 2019; 100, 1039-40.

RP Samy, BG Stiles, G Sethi and LHK Lim. Melioidosis: Clinical impact and public health threat in the tropics. PLOS Neglected Trop. Dis. 2017; 11, e0004738.

S Bongers, P Hellebrekers, LPH Leenen, L Koenderman and F Hietbrink. Intracellular penetration and effects of antibiotics on Staphylococcus aureus inside human neutrophils: A comprehensive review. Antibiotics (Basel, Switzerland) 2019; 8, 54.

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Published

2022-03-15

How to Cite

Prommachote, W., Mala, W. ., Songsri, J. ., Khoosuilee, J. ., Wansu, S. ., Srisara, J. ., Kumkrue, J. ., Phanubol, P. ., & Klangbud, W. K. . (2022). Diversity of Colony Morphotypes, Biochemical Characteristics, and Drug Susceptibility Patterns of Burkholderia pseudomallei Isolated from Humans, Animals, and Environmental Sources in Thailand . Trends in Sciences, 19(8), 153. https://doi.org/10.48048/tis.2022.153

Issue

Vol. 19 No. 8 (2022): Trends in Sciences, Volume 19, Number 8, 15 April 2022

Section

Research Articles

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