The Active Fraction of Physalis angulata Linn. Increases PPARγ and GLUT4 mRNA Expression in an In Vitro Insulin-Resistant C2C12 Cells
DOI:
https://doi.org/10.48048/tis.2026.12840Keywords:
P. angulata active fraction, Insulin resistance, GLUT4, PPAR, Good health and well-being, C2C12 myotubes, Palmitate-induced insulin resistanceAbstract
Insulin resistance, a key feature of type 2 diabetes, is characterized by impaired glucose uptake associated with dysregulation of PPARγ and GLUT4. This study investigated the effects of the active fraction of Physalis angulata Linn. (P. angulata), commonly known as ciplukan, on PPARγ and GLUT4 mRNA expression in an in vitro insulin-resistant C2C12 cell model. Insulin resistance was induced using palmitate, followed by treatment with the active fraction of P. angulata (100 μg/mL) for 4, 12, and 24 h. PPARγ and GLUT4 mRNA expression levels were quantified using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The active fraction of P. angulata significantly increased PPARγ mRNA expression at 4, 12, and 24 h and GLUT4 mRNA expression at 12 h compared with the control group (p < 0.05). These results demonstrate a time-dependent upregulation of genes involved in lipid metabolism and glucose transport in insulin-resistant C2C12 cells. The findings suggest that the active fraction of P. angulata may influence the expression of PPARγ and GLUT4 mRNA in insulin-resistant C2C12 cells, warranting further investigation.
HIGHLIGHTS
- Physalis angulata is traditionally known for its antidiabetic potential.
- Fraction Physalis angulata enhanced PPARγ and GLUT4 gene expression in insulin-resistant C2C12 myotubes.
- The study provides preliminary insight into its potential cellular effects.
GRAPHICAL ABSTRACT
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