Effect of Natural Dietary Formulation LekCapp on the Development of Knee Joint Pain in A Rat Model of Monoiodoacetate-Induced Osteoarthritis
DOI:
https://doi.org/10.48048/tis.2026.11749Keywords:
Herbal supplement, LekCapp, Monoiodoacetate, Knee pain, Osteoarthritis, Herbal supplement, LekCapp, Monoiodoacetate, Knee pain, OsteoarthritisAbstract
In recent years, the use of herbal supplements has risen markedly, with many products readily accessible through online retailers. Despite their growing popularity, scientific evidence supporting their efficacy and safety remains limited. In this study, we investigated the therapeutic potential of a natural product, LekCapp, in alleviating knee pain in a rat model of osteoarthritis (OA). OA knee pain was induced by a unilateral intra-articular injection of 2 mg monoiodoacetate (MIA). LekCapp was administered orally once daily for 14 days following MIA injection, following a preventive treatment protocol. Pain - related behaviors were assessed using weight-bearing distribution and von Frey tests. Histological analysis of knee joints was performed to evaluate potential cartilage-protective effects. Additionally, blood chemical analysis and histological examination of digestive organs were conducted to assess potential systemic toxicity. The results showed that LekCapp significantly reduced knee joint swelling and pain in MIA-treated rats compared with vehicle-treated controls. Although its protective effect against cartilage damage was modest, LekCapp administration preserved normal serum biochemical profiles, and histological evaluation revealed no abnormalities in the livers, kidneys, or stomach. These findings suggest that LekCapp may provide a safe and effective option for relieving OA-related knee pain.
HIGHLIGHTS
- LekCapp reduced knee joint swelling in a rat model of Monoiodoacetate-induced osteoarthritis
- LekCapp produced dose-dependent pain relief in a rat model of MIA-induced knee joint pain.
- LekCapp showed a safety profile after daily administration for 14 days.
GRAPHICAL ABSTRACT
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VP Leifer, JN Katz and E Losina. The burden of OA-health services and economics. Osteoarthritis Cartilage 2022; 30(1), 10-16.
GL Nielsen, HT Sorensen, L Mellemkjoer, WJ Blot, JK McLaughlin, U Tage-Jensen and JH Olsen. Risk of hospitalization resulting from upper gastrointestinal bleeding among patients taking corticosteroids: A register-based cohort study. The American Journal of Medicine 2001; 111(7), 541-545.
Z Zegota, J Gozdzik and J Glogowska-Szelag. Efficacy of herbal and naturally-derived dietary supplements for the management of knee osteoarthritis: A mini-review. Wiadomości Lekarskie 2021; 74(8), 1975-1983.
JM Benson, AK Miller, N Cooper, DN Muanza, JR Smith and DM Shepherd. Anti-inflammatory effects of natural product formulations on murine macrophages. Journal of Dietary Supplements 2010; 7(3), 227-239.
G Honvo, L Lengele, A Charles, JY Reginster and O Bruyere. Role of collagen derivatives in osteoarthritis and cartilage repair: A systematic scoping review with evidence mapping. Rheumatology and Therapy 2020; 7(4), 703-740.
A Prakash and R Baskaran. Acerola, an untapped functional superfruit: A review on latest frontiers. Journal of Food Science and Technology 2018; 55(9), 3373-3384.
B Kulczyński and A Gramza-Michałowska. Goji Berry (Lycium barbarum): Composition and health effects - a Review. Polish Journal of Food and Nutrition Sciences 2016; 66(2), 67-75.
KV Peddada, KV Peddada, SK Shukla, A Mishra and V Verma. Role of curcumin in common musculoskeletal disorders: A review of current laboratory, translational, and clinical data. Orthopaedic Surgery 2015; 7(3), 222-231.
C Feng, M Zhao, L Jiang, Z Hu and X Fan. Mechanism of modified Danggui Sini decoction for knee osteoarthritis based on network pharmacology and molecular docking. Evidence‐Based Complementary and Alternative Medicine 2021; 2021(1), 6680637.
N Sheena, TA Ajith and KK Janardhanan. Anti-inflammatory and anti-nociceptive activities of Ganoderma lucidum occurring in South India. Pharmaceutical Biology 2008; 41(4), 301-304.
SJ Jung, MR Oh, DY Lee, YS Lee, GS Kim, SH Park, SK Han, YO Kim, SJ Yoon and SW Chae. Effect of Ginseng extracts on the improvement of osteopathic and arthritis symptoms in women with osteopenia: A randomized, double-blind, placebo-controlled clinical trial. Nutrients 2021; 13(10), 3352.
HG Jo, CY Baek, Y Hwang, E Baek, C Park, HS Song and D Lee. Investigating the anti-inflammatory, analgesic, and chondroprotective effects of Gynostemma pentaphyllum (Thunb.) Makino in osteoarthritis: An in vitro and in vivo study. International Journal of Molecular Sciences 2024; 25(17), 9594.
D Clemett and KL Goa. Celecoxib: A review of its use in osteoarthritis, rheumatoid arthritis and acute pain. Drugs 2000; 59(4), 957-980.
EA Kockaya, G Selmanoglu, K Kismet and MT Akay. Pathological and biochemical effects of therapeutic and supratherapeutic doses of celecoxib in Wistar albino male rats. Drug and Chemical Toxicology 2010; 33(4), 410-414.
AB Nair and S Jacob. A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy 2016; 7(2), 27-31.
SE Bove, SL Calcaterra, RM Brooker, CM Huber, RE Guzman, PL Juneau, DJ Schrier and KS Kilgore. Weight bearing as a measure of disease progression and efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis. Osteoarthritis and Cartilage 2003; 11(11), 821-830.
SR Chaplan, FW Bach, JW Pogrel, JM Chung and TL Yaksh. Quantitative assessment of tactile allodynia in the rat paw. Journal of Neuroscience Methods 1994; 53(1), 55-63.
IJ Chen, SH Lin and CS Wong. Oral shea nut oil triterpene concentrate supplement ameliorates pain and histological assessment of articular cartilage deterioration in an ACLT injured rat knee osteoarthritis model. PLoS One 2019; 14(4), e0215812.
HJ Mankin, H Dorfman, L Lippiello and A Zarins. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. The Journal of Bone & Joint Surgery 1971; 53(3), 523-537.
S Simoes, R Lopes, MCD Campos, MJ Marruz, MEM da Cruz and L Corvo. Animal models of acute gastric mucosal injury: Macroscopic and microscopic evaluation. Animal Models and Experimental Medicine 2019; 2(2), 121-126.
KE Ibrahim, MG Al-Mutary, AO Bakhiet and HA Khan. Histopathology of the liver, kidney, and spleen of mice exposed to gold nanoparticles. Molecules 2018; 23(8), 1848.
R Wulandari, Sudjadi, S Martono and A Rohman. Liquid chromatography and fourier transform infrared spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extract. Journal of Applied Pharmaceutical Science 2018; 8(9), 107-113.
NK Prabaningdyah, S Riyanto, A Rohman and C Siregar. Application of HPLC and response surface methodology for simultaneous determination of curcumin and desmethoxy curcumin in Curcuma syrup formulation. Journal of Applied Pharmaceutical Science 2017; 7(12), 058-064.
CT Huang, PH Chen, SH Chen, JH Lue and YJ Tsai. Curcumin promotes microglial M2 polarization and suppresses chronic constriction: Injury-induced neuropathic pain in a rat model of peripheral neuropathy. Nutrition 2023; 109, 112004.
X Zhang, Z Guan, X Wang, D Sun, D Wang, Y Li, B Pei, M Ye, J Xu and X Yue. Curcumin alleviates oxaliplatin-induced peripheral neuropathic pain through inhibiting oxidative stress-mediated activation of NF-kappaB and mitigating inflammation. Biological and Pharmaceutical Bulletin 2020; 43(2), 348-355.
S Liu, Q Li, MT Zhang, QL Mao-Ying, LY Hu, GC Wu, WL Mi and YQ Wang. Curcumin ameliorates neuropathic pain by down-regulating spinal IL-1beta via suppressing astroglial NALP1 inflammasome and JAK2-STAT3 signalling. Scientific Reports 2016; 6(1), 28956.
Y Li, Y Zhang, DB Liu, HY Liu, WG Hou and YS Dong. Curcumin attenuates diabetic neuropathic pain by downregulating TNF-alpha in a rat model. International Journal of Medical Sciences 2013; 10(4), 377-381.
Y Jeon, CE Kim, D Jung, K Kwak, S Park, D Lim, S Kim and W Baek. Curcumin could prevent the development of chronic neuropathic pain in rats with peripheral nerve injury. Current Therapeutic Research 2013; 74, 1-4.
YH Kang, HJ Lee, CJ Lee and JS Park. Natural products as sources of novel drug candidates for the pharmacological management of osteoarthritis: A narrative review. Biomolecules & Therapeutics 2019; 27(6), 503-513.
M Thakur, W Rahman, C Hobbs, AH Dickenson and DL Bennett. Characterisation of a peripheral neuropathic component of the rat monoiodoacetate model of osteoarthritis. PLoS One 2012; 7(3), e33730.
K Inomata, K Tsuji, H Onuma, T Hoshino, M Udo, M Akiyama, Y Nakagawa, H Katagiri, K Miyatake, I Sekiya, T Muneta and H Koga. Time course analyses of structural changes in the infrapatellar fat pad and synovial membrane during inflammation-induced persistent pain development in rat knee joint. BMC Musculoskeletal Disorders 2019; 20(1), 8.
Y Zhang and Y Zeng. Curcumin reduces inflammation in knee osteoarthritis rats through blocking TLR4 /MyD88/NF-kappaB signal pathway. Drug Development Research 2019; 80, 353-359.
A Aravinthan, MA Hossain, B Kim, CW Kang, NS Kim, KC Hwang, NS Kim, KC Hwang and JH Kim. Ginsenoside Rb(1) inhibits monoiodoacetate-induced osteoarthritis in postmenopausal rats through prevention of cartilage degradation. Journal of Ginseng Research 2021; 45, 287-294.
RX Zhang, K Ren and R Dubner. Osteoarthritis pain mechanisms: Basic studies in animal models. Osteoarthritis Cartilage 2013; 21(9), 1308-1315.
R Oledzki and J Harasym. Acerola (Malpighia emarginata) anti-inflammatory activity - a review. International Journal of Molecular Science 2024; 25(4), 2089.
H Jiang, L Xu, W Liu, M Xiao, J Ke and X Long. Chronic pain causes peripheral and central responses in MIA-induced TMJOA rats. Cellular and Molecular Neurobiology 2022; 42(5), 1441-1451.
J Fernihough, C Gentry, M Malcangio, A Fox, J Rediske, T Pellas, B Kidd, S Bevan and J Winter. Pain related behaviour in two models of osteoarthritis in the rat knee. Pain 2004; 112(1-2), 83-93.
SM Lockwood, DM Lopes, SB McMahon and AH Dickenson. Characterisation of peripheral and central components of the rat monoiodoacetate model of Osteoarthritis. Osteoarthritis Cartilage 2019; 27(4), 712-722.
D Martinez-Puig, E Costa-Larrion, N Rubio-Rodriguez and P Galvez-Martin. Collagen supplementation for joint health: The link between composition and scientific knowledge. Nutrients 2023; 15(6), 1332.
RL Maynard and N Downes. Liver. In: RL Maynard and N Downes (Eds.). Anatomy and histology of the laboratory rat in toxicology and biomedical research. 1st ed. Academic Press, London, 2019, p. 159-168.
SW Barthhold, SM Griffy and DH Percy. Pathology of laboratory rodents and rabbits. 4th ed. Wiley-Blackwell, New Jersey, United States, 2016. p. 81-106.
JC Seely, GC Hard and B Blankenship. Kidney. In: AW Suttie (Ed.). Boorman’s pathology of the rat. 2nd ed. Academic Press, London, 2018, p.125-166.
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