Ultrasound-Assisted Extraction of L-Tryptophan from Chamomile Flower: Method Development and Application for Flower Parts Characterization and Varietal Difference
DOI:
https://doi.org/10.48048/tis.2024.7348Keywords:
Tryptophan, Optimization, Edible flower, Box-Behnken design, Ray florets, Disc floretsAbstract
Chamomile has been widely used as a functional tea due to its effects on several neurohormones, some of which are related to tryptophan. An analytical ultrasound-assisted extraction (UAE) technique was successfully optimized and validated to determine tryptophan levels in chamomile flowers. A Box-Behnken design, in conjunction with response surface methodology, was conducted based on 3 factors and 3 levels: Temperature (x1; 30, 50, and 70 °C), solvent composition (x2; 0, 40, and 80% methanol in water), and ultrasonic power (x3; 20, 60, and 100%). The main (x2) and quadratic effect of solvent (x2x2); quadratic of temperature (x1x1) and ultrasonic power (x3x3); also, the interaction of solvent-ultrasonic power (x2x3) and temperature-ultrasonic power (x1x3), significantly affected (p < 0.05) the level of L-tryptophan in the extracts. Optimum extraction conditions were achieved by applying a temperature of 54 °C for 15 min using 17% methanol in water and 69% ultrasonic power. A high recovery (94.26%) was achieved with 2 extraction cycles. The coefficient of variation (CV) demonstrated that the developed method had a satisfactory level of precision (CV < 5%) for repeatability and intermediate precision. To check the applicability of the method, the parts of 2 types of chamomile flowers (German and Roman) were evaluated. This method was successfully applied to characterize chamomile flowers based on L-tryptophan levels.
HIGHLIGHTS
- A novel ultrasound-assisted extraction method was successfully optimized and validated for extracting L-tryptophan from chamomile flowers
- Solvent composition was the most influential factor in extracting L-tryptophan from chamomile flowers
- The level of L-tryptophan in ray and disc florets part of Roman dan German chamomile was revealed for the first time
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DM Richard, MA Dawes, CW Mathias, A Acheson, N Hill-Kapturczak and DM Dougherty. L-tryptophan: Basic metabolic functions, behavioral research and therapeutic indications. Int. J. Tryptophan Res. 2009; 2, 45-60.
EL Gibson. Tryptophan supplementation and serotonin function: Genetic variations in behavioural effects. Proc. Nutr. Soc. 2018; 77, 174-88.
A Gallegos and RR Isseroff. Simultaneous determination of tryptophan, 5-Hydroxytryptophan, tryptamine, serotonin, and 5-HIAA in small volumes of mouse serum using UHPLC-ED. MethodsX 2022; 9, 101624.
MM Essa, H Hamdan, SB Chidambaram, B Al-Balushi, GJ Guillemin, M Ojcius and MW Qoronfleh. Possible role of tryptophan and melatonin in covid-19. Int. J. Tryptophan Res. 2020; 13, 1178646920951832.
LAE Erland and P Saxena. Auxin driven indoleamine biosynthesis and the role of tryptophan as an inductive signal in hypericum perforatum (L.). PLos One 2019; 14, e0223878.
X Li, ZH Zhang, HM Zabed, J Yun, G Zhang and X Qi. An insight into the roles of dietary tryptophan and its metabolites in intestinal inflammation and inflammatory bowel disease. Mol. Nutr. Food Res. 2021; 65, e2000461.
HM Roager and TR Licht. Microbial tryptophan catabolites in health and disease. Nat. Comm. 2018; 9, 3294.
K Prabakaran, PJ Jandas, J Luo, C Fu and Q Wei. Molecularly imprinted poly(Methacrylic Acid) based QCM biosensor for selective determination of L-tryptophan. Colloid. Surface. Physicochem. Eng. Aspect. 2021; 611, 125859.
S Ahmad, A Azhar, P Tikmani, H Rafique, A Khan, H Mesiya and HA Saeed. Randomized clinical trial to test efficacy of chamomile and saffron for neuroprotective and anti-inflammatory responses in depressive patients. Heliyon 2022; 8, e10774.
M Adib-Hajbaghery and SN Mousavi. The effects of chamomile extract on sleep quality among elderly people: A clinical trial. Compl. Ther. Med. 2017; 35, 109-14.
T Herrera, Y Aguilera, M Rebollo-Hernanz, E Bravo, V Benítez, N Martínez-Sáez, SM Arribas, MDD Castillo and MA Martín-Cabrejas. Teas and herbal infusions as sources of melatonin and other bioactive non-nutrient components. LWT 2018; 89, 65-73.
A Parraga, J Gonzales, R Portales, C Ruiz and R Rojas. Proximate analysis and aminoacid profiles of leaves, flowers, pods, and seeds of erythrina edulis from Peru. Int. J. Pharm. Pharmaceut. Sci. 2021; 13, 30-2.
R Sayeed, M Thakur and A Gani. Celosia cristata linn. Flowers as a new source of nutraceuticals- a study on nutritional composition, chemical characterization and in-vitro antioxidant capacity. Heliyon 2020; 6, e05792.
MN Qureshi, G Stecher and GK Bonn. Quality control of herbs: Determination of amino acids in althaea officinalis, matricaria chamomilla and taraxacum officinale. Pakistan J. Pharmaceut. Sci. 2014; 27, 459-62.
H Xia and L Qiu. Method for extracting chamomile flavonoid by continuous counter-current ultrasound wave. Xinjiang Bill Brothers Biotechnology Co Ltd, Zhejiang, , China, 2014.
MR González-Centeno, F Comas-Serra, A Femenia, C Rosselló and S Simal. Effect of power ultrasound application on aqueous extraction of phenolic compounds and antioxidant capacity from grape pomace (Vitis Vinifera L.): Experimental kinetics and modeling. Ultrason. Sonochem. 2015; 22, 506-14.
W Setyaningsih, E Duros, M Palma and CG Barroso. Optimization of the ultrasound-assisted extraction of melatonin from red rice (Oryza sativa) grains through a response surface methodology. Appl. Acoust. 2016; 103, 129-35.
W Setyaningsih, IE Saputro, M Palma and CG Barroso. Optimization of the ultrasound-assisted extraction of tryptophan and its derivatives from rice (Oryza sativa) grains through a response surface methodology. J. Cereal Sci. 2017; 75, 192-7.
F Chemat, N Rombaut, AG Sicaire, A Meullemiestre, AS Fabiano-Tixier and M Abert-Vian. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason. Sonochem. 2017; 34, 540-60.
K Kumar, S Srivastav and VS Sharanagat. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrason. Sonochem. 2021; 70, 105325.
VGP Putra, S Mutiarahma, W Chaniago, P Rahmadi, D Kurnianto, C Hidayat, C Carrera, M Palma and W Setyaningsih. An ultrasound-based technique for the analytical extraction of phenolic compounds in red algae. Arabian J. Chem. 2022; 15, 103597.
SLC Ferreira, RE Bruns, HS Ferreira, GD Matos, JM David, GC Brandão, EGPD Silva, LA Portugal, PSD Reis and AS Souza. Box-Behnken design: An alternative for the optimization of analytical methods. Anal. Chim. Acta 2007; 597, 179-86.
Q Liang, J Cui, H Li, J Liu and G Zhao. Florets of sunflower (Helianthus Annuus L.): Potential new sources of dietary fiber and phenolic acids. J. Agr. Food Chem. 2013; 61, 3435-42.
C Carrera, A Ruiz-Rodríguez, M Palma and CG Barroso. Ultrasound-assisted extraction of amino acids from grapes. Ultrason. Sonochem. 2015; 22, 499-505.
H Murashima and A Fujihara. Wavelength dependence of chiral recognition using ions between photoexcited tryptophan and sugars. Chem. Phys. 2020; 536, 110818.
M Spangenberg, JI Bryant, SJ Gibson, PJ Mousley, Y Ramachers and GR Bell. Ultraviolet absorption of contaminants in water. Sci. Rep. 2021; 11, 3682.
ECA. ICH Q2(R1) validation of analytical procedures: Text and methodology. ECA, Mannheim, Germany, 2005.
AOAC International. Appendix F: Guidelines for standard method performance. AOAC International, Maryland, 2016.
E Diraz-Yildirim, S Karaman, M Kulak and A Ilcim. Tryptophan derivatives, phenolic compounds and antioxidant potential of some wild tanacetum taxa from Turkey. Studia Universitatis Babes Bolyai Chemia 2021; 66, 107-22.
W Zhu, Y Fan, Q Xu, X Liu, B Heng, W Yang and Y Hu. Saturated solubility and thermodynamic evaluation of l -tryptophan in eight pure solvents and three groups of binary mixed solvents by the gravimetric method at T = 278.15 - 333.15 K. J. Chem. Eng. Data 2019; 64, 4154-68.
N Medina-Torres, T Ayora-Talavera, H Espinosa-Andrews, A Sánchez-Contreras and N Pacheco. Ultrasound assisted extraction for the recovery of phenolic compounds from vegetable sources. Agronomy 2017; 7, 47.
L Liu, L Jia, W Yang, Y Xiao, J Dai, P Cui, L Zhou and Q Yin. Measurement and correlation of solubility of L-tryptophan in aqueous solutions with a wide range of PH and different monovalent counterions from 283.15 to 323.15 K. J. Solution Chem. 2022; 52, 1-23.
MV Rao, AS Sengar and CK Sunil and A Rawson. Ultrasonication - a green technology extraction technique for spices: A review. Trends Food Sci. Tech. 2021; 116, 975-91.
A Tomšik, B Pavlić, J Vladić, M Ramić, J Brindza and S Vidović. Optimization of ultrasound-assisted extraction of bioactive compounds from wild garlic (Allium Ursinum L.). Ultrason. Sonochem. 2016; 29, 502-11.
S Bellmaine, A Schnellbaecher and A Zimmer. Reactivity and degradation products of tryptophan in solution and proteins. Free Radic. Biol. Med. 2020; 160, 696-718.
FJ Cui, LS Qian, WJ Sun, JS Zhang, Y Yang, N Li, HN Zhuang and D Wu. Ultrasound-assisted extraction of polysaccharides from volvariella volvacea: process optimization and structural characterization. Molecules 2018; 23, 1706.
BA Krizek. Auxin regulation of arabidopsis flower development involves members of the AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) family. J. Exp. Bot. 2011; 62, 3311-9.
N Zoulias, SHC Duttke, H Garcês, V Spencer and M Kim. The role of auxin in the pattern formation of the asteraceae flower head (Capitulum). Plant Physiol. 2019; 179, 391-401.
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