Wound Healing Activity of Transdermal Patches of Carica Papaya, Chromolaena Odorata, and Averrhoa Bilimbi Leaves on Incision Wounds of Hyperglycemic Rat
DOI:
https://doi.org/10.48048/tis.2023.6944Keywords:
Leaves extract, Wistar rats, Transdermal patches, Wound healingAbstract
Transdermal patches have been used for drug delivery to accelerate the wound healing process with minimum negative effect. This study evaluated the wound healing potential of transdermal patches containing Carica papaya, Chromolaena odorata, and Averrhoa bilimbi leaves extract on hyperglycemic rat as a diabetic wound model. For this purpose, a total of 40 Wistar rats aged 2 - 3 months were randomly distributed into 10 groups. The first 5 groups (P1, P2, P3, P4 and P5) consisted of normal rats which received normal dressing, TP Dermafix, TP of C. papaya, TP of C. odorata, and TP of A. bilimbi, respectively. The second 5 groups (P6, P7, P8, P9 and P10) were hyperglycemic rats that received normal dressing, TP Dermafix, TP of C. papaya, TP of C. odorata, and TP of A. bilimbi, respectively. Skin incisions were made perpendicular to the spine in the thickest part of the skin with an incision length of 2 cm and a depth of 0.5 cm. Patches were applied to the incisions according to the test group and replaced every 2 days for a period of 13 days. Wound healing activity was determined by evaluating the Clinical Sign of Inflammation (CSI) score, wound closure, TGF-β1 concentration, and histology of skin tissue. Data were analyzed using oneway analysis of variance (ANOVA). The results showed that for each parameter observed, the rats intervened with transdermal patches loaded with extract leaves had higher healing potential and different from the control group (normal dressing without intervene) both in normal and hyperglycemic rats. This made us concluded that the TP loaded with C. papaya, C. odorata, and A. bilimbi extract leaves have potential as wound healing agents and could be considered as alternate to the synthetic transdermal patches.
HIGHLIGHTS
- The use of drugs-loaded transdermal patches has been widely studied for the treatment of diabetic wounds, however, the potency of one’s containing Carica papaya, Chromolaena odorata, and Averrhoa bilimbi leaf extracts have not been studied. The present study reports the effectiveness of Carica papaya, Chromolaena odorata, and Averrhoa bilimbi impregnated transdermal patches in treating diabetic rat wounds based on CSI score, histological feature of rat skin and TGF- β concentration.
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