Comparison of Essential Amino Acids, Bioactive Compounds and Antioxidants on Fruit Ripening Stage of Lepisanthes rubiginosa (Roxb.) Leenh

Authors

  • Issaraporn Somboonwatthanakul Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Chadaporn Senakun Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
  • Jurairat Kurukod Department of Environmental Education, Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150, Thailand

DOI:

https://doi.org/10.48048/tis.2023.6927

Keywords:

Essential amino acids, Antioxidants, Bioactive compounds, Lepisanthes rubiginosa

Abstract

Essential amino acids, bioactive compounds and antioxidants of Lepisanthes rubiginosa (Roxb.) Leenh were determined on the 3 stages of fruit ripening including unripe, half-ripe and ripe. Eight essential amino acids, identified by LCMS/MS, were found in most samples ranging from 5.63 ± 0.08 to 689.89 ± 25.12 µg/g DW. Glucose, fructose and caffeic acid contents ranged 49.86 ± 0.04 to 258.96 ± 0.86 mg/g, 61.41 ± 0.01 to 259.23 ± 2.71 mg/g and 114.67 ± 0.35 μg/g to 124.65 ± 0.33 μg/g, respectively. Total phenolic content (TPC) and total flavonoid content (TFC) ranged from 9.06 ± 0.22 to 124.65 ± 0.33 17 mg gallic acid equivalent (GAE)/100 g dry weight (DW) and 9.73 ± 0.02 to 105.83 ± 6.63 μg/g DW, respectively. These results showed the first to compare essential amino acids, bioactive compounds and antioxidants in the fruit ripening stages of Lepisanthes rubiginosa. The information of this study indicated promise for further development of functional ingredients in food or cosmetic products.

HIGHLIGHTS

  • These results showed the first to compare essential amino acids, bioactive compounds and antioxidants in the fruit ripening stages of Lepisanthes rubiginosa
  • The highest TFC was found in half-ripe fruits (46.82 ± 1.24 mg RE/g DW), followed by ripe (19.62 ± 0.94 mg RE/g DW) and unripe (6.63 ± 0.02 mg RE/g DW) as shown in Table 1
  • Both qualitative and quantitative LCMS/MS results of 10 amino acids were identified for the first time, including 9 essential amino acids in rubiginosa


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References

JD Dhar, VK Bajpai, BS Setty and VP Kamboj. Morphological changes in human spermatozoa as examined under scanning electron microscope after in vitro exposure to saponins isolated from Sapindus mukorossi. Contraception 1989; 39, 563-8.

SC Chhabra, RLA Mahunnah and EN Mshiu. Plants used in traditional medicine in eastern tanzania. V. Angiosperms (passifloraceae to sapindaceae). J. Ethnopharmacol. 1991; 33, 143-57.

AJ Pirzada, W Shaikh, K Usmanghani and E Mohiuddin. Antifungal activity of Dodonaea viscosa Jacq extract on pathogenic fungi isolated from superficial skin infection. Pakistan J. Pharmaceut. Sci. 2010; 23, 337-40.

R Parganiha, J Mishra, VS Deshmukh, BP Muley, HA Sawarkar and P Kashyap. In vitro anti-asthmatic activity of fruit extract of Sapindus mukorossi. Int. J. Herbs Drug Res. 2011; 1, 11-4.

GHJ Rao and P Lakshmi. Sapindus trifoliates: A review. Int. J. Pharm. Tech. Res. 2012; 4, 2201-14.

TO Adeyemi and OT Ogundipe. Biodiversity of sapindaceae in west africa: A checklist. Int. J. Biodiversity Conservat. 2012; 4, 358-63.

H Kuspradini, D Susanto, Ritmaleni and T Mitsunaga. Phytochemical and comparative study of antimicrobial activity of Lepisanthes amoena leaves extract. J. Biol. Agr. Healthc. 2012; 2, 80-6.

EWM Verheij and RE Coronel. Plant resources of South-East Asia. No. 2: Edible fruits and nuts. Wageningen (Netherlands) Pudoc, Wageningen, Netherlands, 1991.

AA Saburi, MT Martin, B Hill, V Dumontet, MV Tri, T Sevenet and M Pais. Chemical investigation of the methanolic fraction of Lepisanthes rubiginosa bark has led to the isolation and characterisation of a new tetrasaccharide derivative of farnesol named rubiginoside along with known triterpenoid saponins. Phytochemistry 1999; 51, 1039-41.

SA Adesanya, MT Martin, B Hill, V Dumontet, TM Van, T Sévenet and M Païs. Rubiginoside, a farnesyl glycoside from Lepisanthes rubiginosa. Phytochemistry 1999; 51, 1039-41.

J Chuangbunyat, A Teerawutkulrag, SG Pyne, S Liawruangrath and B Liawruangrath. A comparative study of the essential oil from flowers and fruits of Lepisanthes rubiginosa (Roxb.) Leenh. Acta Pharmaceut. Sci. 2011; 53, 535-42.

MM Hasan, A Hossain, A Shamim and RM Mustafizur. Phytochemical and pharmacological evaluation of ethanolic extract of Lepisanthes rubiginosa L. leaves. BMC Compl. Alternative Med. 2017; 17, 496.

KA Wadkar, CS Magdum, SS Patil and NS Naikwade. Anti-diabetic potential and Indian medicinal plants. J. Herbal Med. Toxicol. 2008; 2, 45-50.

V Babu, T Gangadevi and A Subramanian. Antihyperglycemic effect of Cassia keinii leaf extract in glucose feed normal rats and alloxam induced diabetic rats. Indian J. Pharmacol. 2002; 34, 409-15.

R Parthasarathy, R Ilavarasan and CM Karrunakaran. Antidiabetic activity of Thespesia populnea bark and leaf extract against streptozotocin induced diabetic rats. Int. J. PharmTech Res. 2009; 1, 1069-72.

S Das and B De. In vitro inhibition of key enzymes related to diabetes by the aqueous extracts of some fruits of West Bengal, India. Curr. Nutr. Food Sci. 2012; 8, 19-24.

R Sangeetha and N Vedasree. In vitro α-amylase inhibitory activity of the leaves of thespesia populnea. ISRN Pharmacol. 2012; 2012, 515634.

M Telagari and K Hullatti. In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. Extracts and fractions. Indian J. Pharmacol. 2015; 47, 425-9.

OA Fawole and UL Opara. Effects of maturity status on biochemical content, polyphenol composition and antioxidant capacity of pomegranate fruit arils (cv.‘Bhagwa’). S. Afr. J. Bot. 2013; 85, 23-31.

SBI Assanga, LML Luján, EG Rivera-Castañeda, AA Gil-Salido, AL Acosta-Silva, CY Meza-Cueto and JL Rubio-Pino. Effect of maturity and harvest season on antioxidant activity, phenolic compounds and ascorbic acid of Morinda citrifolia L. (noni) grown in Mexico (with track change). Afr. J. Biotechnol. 2013; 12, 4630-9.

R Haruenkit, S Poovarodom, S Vearasilp, J Namiesnik, M Sliwka-Kaszynska, Y Park, B Heo, J Cho, HG Jang and S Gorinstein. Comparison of bioactive compounds, antioxidant and antiproliferative activities of Mon Thong durian during ripening. Food Chem. 2010; 118, 540-7.

B Parikh and VH Patel. Quantification of phenolic compounds and antioxidant capacity of an underutilized Indian fruit: Rayan [Manilkara hexandra (Roxb.) Dubard]. Food Sci. Hum. Wellness 2017; 6, 10-9.

L Butkhup and S Samappito. Changes in physic-chemical properties polyphenol compounds and antiradical activity during development and ripening of MAOLUANG (Antidesma bunius L. Spreng) fruits. J. Fruit Ornamental Plant Res. 2011; 19, 85-99.

L Min, A Huaming and W Daoping. Characterization of amino acid composition in fruits of three Rosa roxburghii genotypes. Horticultural Plant J. 2017; 3, 232-6.

R Caidan, L Cairang, B Liu and Y Suo. Amino acid, fatty acid, and mineral compositions of fruit, stem, leaf and root of Rubus amabilis from the Qinghai-Tibetan Plateau. J. Food Compos. Anal. 2014; 33, 26-31.

R Mandrioli, L Mercolini and RM Augusta. Recent trends in the analysis of amino acids in fruits and derived foodstuffs. Anal. Bioanalytical Chem. 2013; 405, 7941-56.

WW Fish. A reliable methodology for quantitative extraction of fruit and vegetable physiological amino acids and their subsequent analysis with commonly available HPLC systems. Food Nutr. Sci. 2012; 3, 863-71.

F Zeng, J Ou, Y Huang, Q Li, G Xu, Z Liu and S Yang. Determination of 21 free amino acids in fruit juices by HPLC using a modification of the 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) method. Food Anal. Meth. 2015; 8, 428-37.

A Jelled, Â Fernandes, L Barros, H Chahdoura, L Achour, ICFR Ferreira and HB Cheikh. Chemical and antioxidant parameters of dried forms of ginger rhizomes. Ind. Crop. Prod. 2015; 77, 30-5.

J Kubola, S Siriamornpun and N Meeso. Phytochemicals, vitamin C and sugar content of Thai wild fruits. Food Chem. 2011; 126, 972-81.

O Kaisoon, S Siriamornpun, N Weerapreeyakul and N Meeso. Phenolic compounds and antioxidant activities of edible flowers from Thailand. J. Funct. Foods 2011; 3, 88-99.

S Siriamornpun, E Tangkhawanit and N Kaewseejan. Reducing retrogradation and lipid oxidation of normal and glutinous rice flours by adding mango peel powder. Food Chem. 2016; 201, 160-7.

Y Asikin, H Fukunaga, Y Yamano, DX Hou, G Maeda and K Wada. Effect of cultivation line and peeling on food composition, taste characteristic, aroma profile, and antioxidant activity of Shiikuwasha (Citrus depressa Hayata) juice. J. Sci. Food Agr. 2014; 94, 2384-92.

T Chumroenphat, I Somboonwatthanakul, S Saensouk and S Siriamornpun. Changes in curcuminoids and chemical components of turmeric (Curcuma longa L.) under freeze-drying and low-temperature drying methods. Food Chem. 2021; 339, 128121.

MS Nimbalkar, SR Pai, NV Pawar, D Oulkar and GB Dixit. Free amino acid profiling in grain Amaranthus using LC-MS/MS. Food Chem. 2012; 134, 2565-9.

S Siriamornpun, N Weerapreeyakul and S Barusrux. Bioactive compounds and health implications are better for green jujube fruit than for ripe fruit. J. Funct. Foods 2015; 12, 246-55.

N Kumar and N Goel. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol. Rep. 2019; 24, e00370.

A Ullah, S Munir, SL Badshah, N Khan, L Ghani, BG Poulson, AH Emwas and M Jaremko. Important flavonoids and their role as a therapeutic agent. Molecules 2020; 25, 5243.

L Butkhup, W Samappito and S Samappito. Phenolic composition and antioxidant activity of white mulberry (Morus alba L.) fruits. Int J. Food Sci. Tech. 2013; 48, 934-40.

ZFR Ahmed, SSNA Alblooshi, N Kaur, S Maqsood and G Schmeda-Hirschmann. Synergistic effect of preharvest spray application of natural elicitors on storage life and bioactive compounds of date palm (Phoenix dactylifera L., cv. Khesab). Horticulturae 2021; 7, 145.

D Anton, I Bender, T Kaart, M Roasto, M Heinonen, A Luik and T Püssa. Changes in polyphenols contents and antioxidant capacities of organically and conventionally cultivated tomato (Solanum lycopersicum L.) fruits during ripening. Int. J. Anal. Chem. 2017; 2017, 2367453.

MD Vithana, Z Singh, SK Johnson and R Gupta. Concentrations of health-promoting phytochemicals in ripe mango fruit triggered by postharvest application of elicitors. J. Sci. Food Agr. 2019; 99, 1126-34.

RHA Aldhanhani, ZFR Ahmed, N Tzortzakis and Z Singh. Maturity stage at harvest influences antioxidant phytochemicals and antibacterial activity of jujube fruit (Ziziphus mauritiana Lamk. and Ziziphus spina-christi L.). Ann. Agr. Sci. 2022; 67, 196-203.

S Saensouk, R Senavongse, C Papayrata and T Chumroenphat. Evaluation of color, phytochemical compounds and antioxidant activities of mulberry fruit (Morus alba L.) during ripening. Horticulturae 2022; 8, 1146.

M Castrillo, NJ Kruger and FR Whatley. Sucrose metabolism in mango fruit during ripening. Plant Sci. 1992; 84, 45-51.

T Ivancic, J Jakopic, R Veberic, V Vesel and M Hudina. Effect of ripening on the phenolic and sugar contents in the meso- and epicarp of olive fruits (Olea europaea L.) cultivar ‘Leccino’. Agriculture 2022; 12, 1347.

H Manninen, M Rotola-Pukkila, H Aisala, A Hopia and T Laaksonen. Free amino acids and 5′- nucleotides in Finnish forest mushrooms. Food Chem. 2018; 247, 23-8.

A Pęksa, J Miedzianka and A Nemś. Amino acid composition of flesh-coloured potatoes as affected by storage conditions. Food Chem. 2018; 266, 335-42.

A Sorrequieta, G Ferraro, SB Boggio and EM Valle. Free amino acid production during tomato fruit ripening: A focus on l-glutamate. Amino Acids 2010; 38, 1523-32.

SB Boggio, JF Palatnik, HW Heldt and EM Valle. Changes in amino acid composition and nitrogen metabolizing enzymes in ripening fruits of Lycopersicon Esculentum Mill. Plant Sci. 2000; 159, 125-33.

J Mann and S Truswell. Essentials of human nutrition. Oxford University Press, Oxford, 2071.

H Zhang, J Pu, Y Tang, M Wang, K Tian, Y Wang, X Luo and Q Deng. Changes in phenolic compounds and antioxidant activity during development of ‘Qiangcuili’ and ‘Cuihongli’ Fruit. Foods 2022; 11, 3198.

W Kim and WJ Wilbur. Amino acid residue environments and predictions of residue type. Comput. Chem. 2017; 25, 411-22.

GL Cowie and JI Hedges. Sources and reactivities of amino acids in a coastal marine environment. Limnol. Oceanogr. 1992; 37, 703-24.

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Published

2023-09-01

How to Cite

Somboonwatthanakul, I. ., Senakun, C. ., & Kurukod, J. . (2023). Comparison of Essential Amino Acids, Bioactive Compounds and Antioxidants on Fruit Ripening Stage of Lepisanthes rubiginosa (Roxb.) Leenh. Trends in Sciences, 20(11), 6927. https://doi.org/10.48048/tis.2023.6927