The Potential of West Sumatran Dadiah as The Novel to Alleviate Hyperglycemia, Hypercholesterolemia, and Reducing NF-kB Expression in Nephropathy Diabetes Rat Model
DOI:
https://doi.org/10.48048/tis.2023.6812Keywords:
Dadiah, hyperglycemia, Hypercholesterolemia, NF-kB, Diabetes neuropathy, ProbioticAbstract
Diabetic nephropathy (DN) is the most common microvascular complication in diabetes mellitus (DM) patients. The main mechanism for the development of DN is an inflammatory reaction as indicated by increased expression of NF-kB in kidney tissue due to chronic hyperglycemia and hypercholesterolemia. Hyperglycemia is related to changes in the composition of the microbiota which can cause dysbiosis. The therapeutic approach in DM sufferers using probiotics needs attention. Dadiah is a naturally fermented buffalo milk product in bamboo tubes. Even though this probiotic has health benefits, its mechanism as an antidiabetic is not widely known. This study aims to reduce blood sugar, cholesterol, and inflammatory marker NF-kB levels after Dadiah treatment. This study was an experimental animal study using 7 groups, each group of 6 rats, 8 weeks of intervention, and evaluation of serum glucose and cholesterol every 2 weeks. NF-kB expression in kidney tissue was examined after 8 weeks of the termination phase. We examined their effect on blood glucose levels, serum cholesterol, and NF-kB antibody expression in kidney tissue using immunohistochemical assays. probiotic metabolites in intraperitoneal Alloxan-induced diabetic rats 100 mg/kg b. w. The results showed the potential of Dadiah and its metabolites to significantly (one-way ANOVA test) reduce hyperglycemia and serum cholesterol and inhibit oxidative stress by reducing NF-kB expression in kidney tissue after treatment. Dadiah probiotics should be considered as a nutritional companion in DN and as a future therapeutic target for DM patients to prevent the development of microvascular complications and hypercholesterolemia.
HIGHLIGHTS
Dadiah is a naturally fermented buffalo milk product in bamboo tubes. Dadiah (Probiotic) originating from West Sumatra, Indonesia acts as an antidiabetic. Dadiah and its metabolites significantly reduced hyperglycemia and serum cholesterol and inhibited oxidative stress by reducing NF-kB expression in kidney tissue after treatment. Dadiah probiotics should be considered as a nutritional companion in diabetic nephropathy and as a future therapeutic target for DM patients to prevent the development of microvascular complications and hypercholesterolemia.
GRAPHICAL ABSTRACT
Downloads
Metrics
References
RE Pérez-Morales, MDD Pino, JM Valdivielso, A Ortiz, C Mora-Fernández and JF Navarro-González. Inflammation in diabetic kidney disease. Nephron 2019; 143, 12-6.
EC Westman. Type 2 diabetes mellitus: A pathophysiologic perspective. Front. Nutr. 2021; 8, 707371.
M Li, Q Guo, H Cai, H Wang, Z Ma and X Zhang. miR218 regulates diabetic nephropathy via targeting IKK‐β and modulating NKκB‐mediated inflammation. J. Cell. Physiol. 2020; 235, 3362-71.
W Yu, J Shang, R Guo, F Zhang, W Zhang, Y Zhang, F Wu, H Ren, C Liu, J Xiao and Z Zhaoa. The gut microbiome in differential diagnosis of diabetic kidney disease and membranous nephropathy. Ren. Fail. 2020; 42, 1100-10.
H Sawaf, G Thomas, JJ Taliercio, G Nakhoul, TJ Vachharajani and A Mehdi. Therapeutic advances in diabetic nephropathy. J. Clin. Med. 2022; 11, 378.
M Ruiz-Ortega, RR Rodrigues-Diez, C Lavoz and S Rayego-Mateos. Special issue ‘diabetic nephropathy: Diagnosis, prevention and treatment. J. Clin. Med. 2020; 9, 813.
T Kocher, J König, WS Borgnakke, C Pink and P Meisel. Periodontal complications of hyperglycemia/diabetes mellitus: Epidemiologic complexity and clinical challenge. Periodontology 2000 2018; 78, 59-97.
RJ Galindo, M Fayfman and GE Umpierrez. Perioperative management of hyperglycemia and diabetes in cardiac surgery patients. Endocrinol. Metabol. Clin. N. Am. 2018; 47, 203-22.
G Davı̀, P Gresele, F Violi, S Basili, M Catalano, C Giammarresi, R Volpato, GG Nenci, G Ciabattoni and C Patrono. Diabetes mellitus, hypercholesterolemia, and hypertension but not vascular disease Per Se Are associated with persistent platelet activation in vivo: Evidence derived from the study of peripheral arterial disease. Circulation 1997; 96, 69-75.
EBM Daliri, Y Kim, Y Do, R Chelliah and DH Oh. In vitro and in vivo cholesterol reducing ability and safety of probiotic candidates isolated from korean fermented soya beans. Probiotics Antimicrob. Protein. 2022; 14, 87-98.
T Rittiphairoj, K Pongpirul, K Janchot, NT Mueller and T Li. Probiotics contribute to glycemic control in patients with type 2 diabetes mellitus: A systematic review and meta-analysis. Adv. Nutr. 2021; 12, 722-34.
D Xavier-Santos, R Bedani, ED Lima and SMI Saad. Impact of probiotics and prebiotics targeting metabolic syndrome. J. Funct. Foods 2020; 64, 103666.
M Shimizu, M Hashiguchi, T Shiga, H Tamura and M Mochizuki. Meta-analysis: Effects of probiotic supplementation on lipid profiles in normal to mildly hypercholesterolemic individuals. PLos One 2015; 10, e0139795.
Z Guo, XM Liu, QX Zhang, Z Shen, FW Tian, H Zhang, ZH Sun, HP Zhang and W Chen.Influence of consumption of probiotics on the plasma lipid profile: A meta-analysis of randomised controlled trials. Nutr. Metabol. Cardiovasc. Dis. 2011; 21, 844-50.
A Ataie-Jafari, B Larijani, HA Majd and F Tahbaz. Cholesterol-lowering effect of probiotic yogurt in comparison with ordinary yogurt in mildly to moderately hypercholesterolemic subjects. Ann. Nutr. Metabol. 2009; 54, 22-7.
S Usmiati and R Risfaheri. Pengembangan dadih sebagai pangan fungsional probiotik asli Sumatera Barat. Jurnal Penelitian Pengembangan Pertanian 2013; 32, 20-9.
E Purwati, SN Aritonang, S Melia, I Juliyarsi and H Purwanto. Manfaat probiotik bakteri asam laktat dadiah menunjang kesehatan masyarakat. Lembaga Pengembangan Teknologi Informasi dan Komunikasi, Padang, Indonesia, 2017.
A Kundu, P Dey, P Sarkar, S Karmakar, IH Tae, KS Kim, JH Park, SH Lee, BM Lee, L Renthlei, Z Puia and HS Kim. Protective effects of Croton hookeri on streptozotocin-induced diabetic nephropathy. Food Chem. Toxicol. 2020; 135, 110873.
R Amelia, K Philip, YE Pratama and E Purwati. Characterization and probiotic potential of lactic acid bacteria isolated from dadiah sampled in West Sumatra. Food Sci. Tech. 2021; 41, 746-52.
Y Wang, D Dilidaxi, Y Wu, J Sailike, X Sun and X Nabi. Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice. Biomed. Pharmacother. 2020; 125, 109914.
H Harun, Y Yanwirasti, B Purwanto and EP Rahayuningsih. The effect of giving dadih on malondialdehyde levels and renal interstitial fibrosis at aging kidney. Open Access Macedonian J. Med. Sci. 2020; 8, 293-6.
X Guo, S Hong, H He, Y Zheng, Y Chen, X Mo, J Li, L Li, R Steinmetz and Q Liu. NFκB promotes oxidative stress-induced necrosis and ischemia/reperfusion injury by inhibiting Nrf2-ARE pathway. Free Radic. Biol. Med. 2020; 159, 125-35.
M Miraghajani, N Zaghian, M Mirlohi, A Feizi and R Ghiasvand. The impact of probiotic soy milk consumption on oxidative stress among type 2 diabetic kidney disease patients: A randomized controlled clinical trial. J. Ren. Nutr. 2017; 27, 317-24.
Y Zheng, SH Ley and FB Hu. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat. Rev. Endocrinol. 2018; 14, 88-98.
L Kong, H Wu, W Zhou, M Luo, Y Tan, L Miao and L Cai. Sirtuin 1: A target for kidney diseases. Mol. Med. 2015; 21, 87-97.
A Guilbaud, M Howsam, C Niquet-Leridon, F Delguste, M Fremont, S Lestavel, P Maboudou, A Garat, S Schraen, B Onraed, B Foligné, É Boulanger and FJ Tessier. The effect of Lactobacillus fermentum ME‐3 treatment on glycation and diabetes complications. Mol. Nutr. Food Res. 2020; 64, 1901018.
AA Dumas, A Lapointe, M Dugrenier, V Provencher, B Lamarche and S Desroches. A systematic review of the effect of yogurt consumption on chronic diseases risk markers in adults. Eur. J. Nutr. 2017; 56, 1375-92.
H Zhong, Abdullah, L Deng, M Zhao, J Tang, T Liu, H Zhang and F Feng. Probiotic-fermented blueberry juice prevents obesity and hyperglycemia in high fat diet-fed mice in association with modulating the gut microbiota. Food Funct. 2020; 11, 9192-207.
E Amirani, Z Asemi and M Taghizadeh. The effects of selenium plus probiotics supplementation on glycemic status and serum lipoproteins in patients with gestational diabetes mellitus: A randomized, double-blind, placebo-controlled trial. Clin. Nutr. ESPEN 2022; 48, 56-62.
L Wang, M Guo, Q Gao, J Yang, L Yang and X Juang. The effects of probiotics on total cholesterol: A meta-analysis of randomized controlled trials. Medicine 2018; 97, e9679.
SM Lim, JJ Jeong, KH Woo, MJ Han and DH Kim. Lactobacillus sakei OK67 ameliorates high-fat diet-induced blood glucose intolerance and obesity in mice by inhibiting gut microbiota lipopolysaccharide production and inducing colon tight junction protein expression. Nutr. Res. 2016; 36, 337-48.
L Wu, Q Wang, F Guo, X Ma, J Wang, Y Zhao, Y Yan and G Qin. Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress. J. Cell. Physiol. 2021; 236, 1454-68.
A Khare and S Gaur. Cholesterol-lowering effects of lactobacillus species. Curr. Microbiol. 2020; 77, 638-44.
HJ Zheng, J Guo, Q Jia, YS Huang, H Wei-Jun, W Zhang, F Zhang, WJ Liu and Y Wang. The effect of probiotic and synbiotic supplementation on biomarkers of inflammation and oxidative stress in diabetic patients: A systematic review and meta-analysis of randomized controlled trials. Pharmacol. Res. 2019; 142, 303-13.
B Bhat and BK Bajaj. Multifarious cholesterol lowering potential of lactic acid bacteria equipped with desired probiotic functional attributes. 3 Biotech 2020; 10, 200.
L Merone and R McDermott. Nutritional anti-inflammatories in the treatment and prevention of type 2 diabetes mellitus and the metabolic syndrome. Diabetes Res. Clin. Pract. 2017; 127, 238-53.
B Xue, J Yu, J Zhang, F Hao, X Zhang, J Dong, J Sun and J Xue. Microbial diversity analysis of vineyard son the eastern foothills of the Helan Mountain region using high-throughput sequencing. Food Sci. Tech. 2022; 42, e66320.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Walailak University
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
