Formulation and Phytochemical Profile of a Product Prototype Infused with Cannabis sativa Leaves

Authors

  • Pongsathorn Tongkasee Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Dutsdee Srithat Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Patcharawalai Sriyasak Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Anunkaorn Jitcharerntham Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Teera Piwgern Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Jarinya Khoontawad Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand
  • Wilaiporn Insuwan Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin 32000, Thailand

DOI:

https://doi.org/10.48048/tis.2023.5835

Keywords:

Marijuana, Phytochemical, Antioxidant activity, Cannabis, Physiochemical analysis

Abstract

This study investigated the formulation of a product prototype RMUTI-SKC by using the legal part of cannabis plants (leaves). Five product prototypes were composed of cannabis (leaves), stevia and mint. The herbs were added to improve the taste, smell and physical appearance properties of the product prototype which was yellowish-green.  The analysis of the chemical properties found no heavy metals (Cd, As, and Pb) were observed (based on THAI community product standard, and demonstrated a moderate antioxidant activity, total phenolic content and total flavonoid content. The GC-MS profile of crude extracts analysis revealed the phytochemicals based on pharmacological action of essential oil, flavoring substance, anti-inflammatory, antioxidant, anti-bacterial, antidiabetic, antifungal and anti-cancer, respectively. The experimental results showed the pharmacological properties of the product prototype which can be a guideline for applying cannabis plants to other products. Further study on pathogen testing and product aging should be carried out to satisfy the quality of production.

HIGHLIGHTS

  • The formulation of a product prototype RMUTI-SKC by using the legal part of cannabis plants (leaves). The analysis of the chemical properties found no heavy metals (Cd, As and Pb) were observed (based on THAI community product standard, and demonstrated a moderate antioxidant activity, total phenolic content and total flavonoid content.


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References

D Gloss. An overview of products and bias in research. Neurotherapeutics 2015; 12, 731-4.

United Nations. Single convention on narcotic drugs, 1961, Available at: https://www.unodc. org/pdf/convention_1961 _en.pdf, accessed May 2022.

BK Madras. Update of Cannabis and its medical use. In: Proceedings of the 37th Expert Committee on Drug Dependence, Geneva, Switzerland. 2015, p. 1-41.

R Pertwee. Handbook of Cannabis, Available at: http://www.oxfordscholarship.com/view/ 10.1093/acprof:oso/9780199662685.001.0001/acprof-9780199662685, accessed May 2022.

ND Volkow, WM Compton and SRB Weiss. Adverse health effects of marijuana use. New Engl. J. Med. 2014; 370, 2219-27.

RJM Niesink and MWV Laar. Does cannabidiol protect against adverse psychological effects of THC? Front. Psychiatr. 2013; 4, 130.

G Appendino, S Gibbons, A Giana, A Pagani, G Grassi, M Stavri, E Smith and MM Rahman. Antibacterial cannabinoids from cannabis sativa: A structure-activity study. J. Nat. Prod. 2008; 71, 1427-30.

R Brenneisen. Chemistry and analysis of phytocannabinoids and other cannabis constituents. Marijuana and the cannabinoids. Humana Press, New Jersey, 2007, p. 17-49.

M Fellermeier and MH Zenk. Prenylation of olivetolate by a hemp transferase yields cannabigerolic acid, the precursor of tetrahydrocannabinol. FEBS Lett. 1998; 427, 283-5.

I Nahtigal, A Blake and A Hand. The pharmacological properties of cannabis. J. Pain Symptom Manag. 2016; 9, 481-91.

G Lafaye, L Karila, L Blecha and MDA Benyamina. Cannabis, cannabinoids, and health. Dialogues Clin. Neurosci. 2017; 19, 309-16.

F Brahmi, M Khodir, C Mohamed and D Pierre. Chemical composition and biological activities of Mentha species. In: HA El-Shemy (Ed.). Aromatic and Medicinal Plants. IntechOpen, London, 2017.

TC Silva, SKV Bertolucci, AA Carvalho, WN Tostes, ICA Alvarenga, FV Pacheco, RMAD Assis, ADC Honorato and JEBP Pinto. Macroelement omission in hydroponic systems changes plant growth and chemical composition of Melissa officinalis L. essential oil. J. Appl. Res. Med. Aromat. Plants 2021; 24, 100297.

MVSD Andrade, RDD Castro, DDS Cunha, VG Neto, MGA Carosio, AG Ferreira, LCD Souza-Neta, LG Fernandez and PR Ribeiro. Stevia rebaudiana (Bert.) Bertoni cultivated under different photoperiod conditions: Improving physiological and biochemical traits for industrial applications. Ind. Crop. Prod. 2021; 168, 113595.

A Jarma-Orozco, E Combatt-Caballero and J Jaraba-Navas. Growth and development of Stevia rebaudiana Bert., in high and low levels of radiation. Curr. Plant Biol. 2020; 22, 100144.

E Díaz-Montes, P Gutiérrez-Macías, C Orozco-Álvarez and R Castro-Muñoz. Fractionation of Stevia rebaudiana aqueous extracts via two-step ultrafiltration process: Towards rebaudioside a extraction. Food Bioproducts Process. 2020; 123, 111-22.

M Pakkirisamy, SK Kalakandan and K Ravichandran. Phytochemical screening, GC-MS, FT-IR analysis of methanolic extract of curcuma caesia Roxb (black turmeric). Phcog. J. 2017; 9, 952-6.

C Cabrera, R Artacho and R Gimenez. Beneficial effects of green tea: A review. J. Am. Coll. Nutr. 2006; 25, 79-99.

A Srisopa and K Wongkrajang. Development of antioxidant and anti-α-glucosidase mulberry leaf tea recipes with combination of aroma herbs. Thai J. Sci. Tech. 2019; 9, 219-29.

NA Ibraheem, MM Hasan, RZ Khan and PK Mishra. Understanding color models: A review. ARPN J. Sci. Tech. 2012; 2, 265-75.

C Kornpointner, AS Martinez, S Marinovic, C Haselmair-Gosch, P Jamnik, K Schroder, C Lofke and H Halbwirth. Chemical composition and antioxidant potential of Cannabis sativa L. roots. Ind. Crop. Prod. 2021; 165, 113422.

Z Jemmali, A Chartier and C Elkafir. Development of a gas chromatography-mass spectrometry method to monitor in a single run, mono- to triterpenoid compounds distribution in resinous plant materials. J. Chrom. 2016; 1443, 241-53.

M Tuzen and M Soylak. Evaluation of metal levels of drinking waters from the Tokat-Black Sea region of Turkey. Pol. J. Environ. Stud. 2006; 15, 915-9.

A Turek, K Wieczorek and WM Wolf. Digestion procedure and determination of heavy metals in sewage sludge - an analytical problem. Sustainability 2019; 11, 1753.

F Adamu, M Metto and B Kassie. Determination of heavy metals in soil used for potato cultivation by atomic absorption spectroscopy in awi Zone, Amhara Region, Ethiopia. MOJ Ecol. Environ. Sci. 2021; 6, 28-33.

A Shrivastava and VB Gupta. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles Young Sci. 2011; 2, 21-5.

DP Singh, S Verma and R Prabha. Investigations on antioxidant potential of phenolic acids and flavonoids: The common phytochemical ingredients in plants. J. Plant Biochem. Physiol. 2018; 6, 1000219.

A Oubihi, H Hosni, I Nounah, A Ettouil, H Harhar, K Alaoui, M Ouhssine and Z Guessous. Phenolic content, antioxidant activity, anti-inflammatory potential, and acute toxicity study of Thymus leptobotrys Murb. Extracts. Biochem. Res. Int. 2020; 2020, 8823209.

I Nounah, A Hajib, A Oubihi, H Harhar, S Gharby, B Kartah, Z Charrouf and K Bougrin. Phytochemical screening and biological activity of leaves and stems extract of Hammada Scoparia. Moroccan J. Chem. 2019; 7, 1-9.

XQ Chen, ZH Li, LL Liu, H Wang, SH Yang, JS Zhan and Y Zhang. Green extraction using deep eutectic solvents and antioxidant activities of flavonoids from two fruits of Rubia species. LWT 2021; 148, 111708.

C Kamunda, M Mathuthu and M Madhuku. Health risk assessment of heavy metals in soils from Witwatersrand Gold Mining Basin, South Africa. Int. J. Environ. Res. Publ. Health 2016; 13, 663.

X Zhu, Q Li, J Li, J Luo, W Chen and X Li. Comparative study of volatile compounds in the fruit of two banana cultivars at different ripening stages. Molecules 2018; 23, 2456.

M Adnan, MNU Chy, ATMM Kamal, MOK Azad, A Paul, SB Uddin, JW Barlow, MO Faruque, CH Park and DH Cho. Investigation of the biological activities and characterization of bioactive constituents of Ophiorrhiza rugosa var. prostrata (D.Don) & mondal leaves through in vivo, in vitro, and in silico approaches. Molecules 2019; 24, 1367.

E Wang, X Liu, Z Si, X Li, J Bi, W Dong, M Chen, S Wang, J Zhang, A Song and F Fan. Volatile organic compounds from rice rhizosphere bacteria inhibit growth of the pathogen Rhizoctonia solani. Agriculture 2021; 11, 368.

E Idm’hand, F Msanda and K Cherifi. Medicinal uses, phytochemistry and pharmacology of Ammodaucus leucotrichus. Clin. Phytoscience 2020; 6, 6.

GE Egbung, C Anosike, AB Utu-Baku, I Ogar and VU Nna. Phytochemical evaluation and GC-MS analysis of Hyptis verticillata cultivated in Calabar cross river State, Nigeria. Int. J. Biol. Chem. Sci. 2017; 11, 2548-59.

European Food Safety Authority. Scientific opinion on flavouring group evaluation 3, revision 2 (FGE.03Rev2): Acetals of branched-and straight-chain aliphatic saturated primary alcohols and branched- and straight-chain saturated or unsaturated aldehydes, an ester of a hemiacetal and an orthoester of formic acid, from chemical groups 1, 2 and 4. EFSA J. 2011; 9, 231.

UA Godwin and EF Margaret. Essential oils and fatty acids composition of dry fruits of Tetrapleura tetraptera. J. Appl. Sci. Environ. Manag. 2014; 18, 419-24.

RS Verma, RK Verma, AK Yadav and A Chauhan. Change in the essential oil composition of rose-scented geranium (Pelargonium graveolens L’Herit. ex Ait.) due to date of transplanting under hill conditions of Uttarakhand. Indian J. Nat. Prod. Res. 2010; 1, 367-70.

A Gallardo, MI Picollo, P González-Audino and G Mougabure-Cueto. Insecticidal activity of individual and mixed monoterpenoids of geranium essential oil against Pediculus humanus capitis (Phthiraptera: Pediculidae). J. Med. Entomol. 2012; 49, 332-5.

M Vinutha and KJT Saraswathi. Study on the essential oil of aerial and sub-aerial parts of Cymbopogon flexuosus (Nees ex Steud) Wats. Int. J. Curr. Sci. 2013; 7, 42-7.

Thai Industrial Standards Institute. Thai Community product standard Instant mixed herbs drink, Thailand patent. Ministry of Industry, Thailand, 2013.

MDP Rada-Mendoza, MDM Villamiel-Guerra, OL Hoyos-Saavedra and LF Alvira. Quantification of lead using atomic absorption spectrometry in thermoformed and biodegradable flexible films made from cassava (Manihot esculenta crantz). Dyna 2018; 85, 236-42.

N Eka, Astuti, S Retno and A Rohman. Validation and quantitative analysis of cadmium and lead in snake fruit by flame atomic absorption spectrophotometry. Int. Food Res. J. 2012; 19, 937-40.

AG González and MA Herrador. A practical guide to analytical method validation, including measurement uncertainty and accuracy profiles. Trends Anal. Chem. 2007; 26, 227-38.

S Phongpaichit, J Nikom, N Rungjindamai, J Sakayaroj, N Hutadilok-Towatana, V Rukachaisirikul and K Kirtikara. Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunol. Med. Microbiol. 2007; 51, 517-25.

MA Soobrattee, VS Neergheen, A Luximon-Ramma, OI Aruoma and T Bahorun. Phenolics as potential antioxidant thera-peutic agents: Mechanism and actions. Mutat. Res. Fund. Mol. Mech. Mutagen. 2005; 579, 200-13.

SA Baba and SA Malik. Determination of total phenolic and flavonoid content, antimicrobialand antioxidant activity of a root extract of Arisaema jacquemontii Blume. J. Taibah Univ. Sci. 2015; 9, 449-54.

M Ahmed, M Ji, P Gin, Z Gu, Y Liu, A Sikandar, MF Iqbal and A Javeed. Phytochemical screening, total phenolic and flavonoids contents and antioxidant activities of Citrullus Colocynthis L. and Cannabis Sativa L. Appl. Ecol. Environ. Res. 2019; 17, 6961-79.

S Kamtekar, V Keer and V Patil. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. J. Appl. Pharmaceut. Sci. 2014; 4, 61-5.

Z Drinic, S Vidovic, J Vladic´, A Koren, B Kiprovski and V Sikora. Effect of extraction solvent on total polyphenols content and antioxidant activity of industrial hemp (Cannabis sativa L.). Lekovite Sirovine 2018; 38, 17-21.

PG Mahlberg and ES Kim. Accumulation of cannabinoids in glandular trichomes of cannabis (Cannabaceae). J. Ind. Hemp 2004; 9, 15-36.

M Atanassova, S Georgieva and K Ivancheva. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. J. Univ. Chem. Tech. Metall. 2011; 46, 81-8.

B Havsteen. Flavonoids, a class of natural products of high pharmacological potency. Biochem. Pharmacol. 1983; 32, 1141-8.

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Published

2023-04-29

How to Cite

Tongkasee, P. ., Srithat, D. ., Sriyasak, P. ., Jitcharerntham, A. ., Piwgern, T. ., Khoontawad, J. ., & Insuwan, W. . (2023). Formulation and Phytochemical Profile of a Product Prototype Infused with Cannabis sativa Leaves. Trends in Sciences, 20(9), 5835. https://doi.org/10.48048/tis.2023.5835

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