The Infectivity Survival and Transmissibility of Rice ragged stunt virus from the Frozen-Infected Rice Leaves by the Brown Planthopper, Nilaparvata lugens Stål
DOI:
https://doi.org/10.48048/tis.2022.5097Keywords:
Rice ragged stunt virus, Brown planthopper, Infectivity survival, Indirect NCM-ELISA, Minimal latent periodAbstract
Rice ragged stunt virus, which was transmitted by the brown planthopper (Nilaparvata lugens Stål), was an economically important rice plant pathogen, and caused the yield losses of 10 - 100 % in Thailand and Asian rice cultivation areas. The purpose of this research was to assess the infectivity survival and the transmissibility of Rice ragged stunt virus from the frozen infected rice leaf tissues to the hosts, and the efficiency detection by the indirect enzyme-linked immunosorbent assay on nitrocellulose membranes. The result was shown that rice virus was successfully acquired from the infected rice leaf frozen samples at –20 °C (7 days and 10 months) by the insect vectors, which the reactions can be clearly detected on the nitrocellulose membranes. The efficiencies of the insect vector inoculated, and the rice plant transmitted were 60 - 100 % and 20 - 100 %, and the direct variations with increasing of the viral-minimal latent period at the 3 and 10 days after inoculation, respectively. The maximum efficiency detection of crude sap sample dilutions of both viral hosts in the insect vector and the rice plant were the 1:16 to 1:64, and 1:64 to 1:256, respectively. These new idea transmission methods can be applied for the long-term stability, viability and infectivity of the infected samples, the laboratory protocol development of the cryopreservation techniques for the future study purpose of a plant virus, the viral-resistance breeding process, and the impose rice plant protection policies and strategies in Thailand.
HIGHLIGHTS
- Rice ragged stunt virus (RRSV) causes the most destructive rice plant viral disease in Thailand irrigational rice cultivation
- The brown planthopper (BPH) is an economically important vector and a serious management problem for Thai farmers
- The infected rice leaf samples under the frozen and refrigeration conditions were successful as active reservoirs for the viral cycle and transmission
- The RRSV-minimal latent periods (MLP) of the BPH vector and the rice plant were 3 and 10 days after inoculation
- Understanding of plant-virus-vector interactions can take to improve the ability to insect and rice viral disease control policies and strategies in the agro-ecosystem
GRAPHICAL ABSTRACT
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