Evaluation of Pre-ingestive Citronella Residues using Ruminal In Vitro Techniques

Authors

  • Elihasridas Elihasridas Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia
  • Mardiati Zain Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia
  • Rusmana Wijaya Setia Ningrat Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia
  • Erpomen Erpomen Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia
  • Malik Makmur Post-Doctoral Researcher, Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia
  • Ezi Masdia Putri Graduate Student, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia
  • Halimatuddini Halimatuddini Graduate Student, Faculty of Animal Science, Andalas University, Kampus Limau Manis, Padang, Indonesia

DOI:

https://doi.org/10.48048/tis.2022.5096

Keywords:

Ammonia, Citronella residues, Evaluation, Fermentation, In vitro rumen, Methane

Abstract

Citronella residues (CR) have the potential to be used as an alternative to the fiber diet of ruminants. This study reports on the effects of pre-ingestive CR using ammonia (4 % dry matter (DM)) and fermentation (6 % DM) on in vitro rumen fermentation, metabolism, and methane (CH4) production. Four CR levels of 0, 25, 75, and 100 % DM were used. Each level was repeated 3 times and a complete randomized design method was used. The results showed that pre-ingestive CR significantly increased the pH rumen fluid but decreased ammonia-nitrogen concentration, total iso-volatile fatty acid (iso-VFA) production, and protozoa population (p < 0.01). The pre-ingestive CR significantly decreased the acetic acid composition and rumen microbial protein synthesis (p < 0.05), and significantly increased the proportion of propionic acid, n-butyric acid, and iso-valeric acid (p < 0.05). The total VFA production and rumen CH4 production did not significantly change (p > 0.05). In conclusion, pre-ingestive CR was compatible as a basal diet for ruminants.

HIGHLIGHTS

  • Pre-ingestive treated citronella residues used as substitute in ruminant fiber diet
  • 100 % pre-ingestive citronella residues increased rumen fluid pH compared with napier grass
  • 100 % pre-ingestive citronella residues reduced NH3, VFA, and protozoa
  • 100 % pre-ingestive citronella residues had no effect on methane production
  • Rumen fermentation profile of pre-ingestive residue treatments are suitable alternative diets


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References

WD Clayton, MS Vorontsova, KT Harman and H Williamson. Grassbase - the online world grass flora, Available at: http://www.kew.org/data/grasses-db.html, accessed September 2020.

NA Saputra, HS Wibisono, S Darmawan and G Pari. Chemical composition of Cymbopogon nardus essential oil and its broad spectrum benefit. IOP Conf. Ser. Earth Environ. Sci. 2020; 415, 012017.

A Sulaswatty, MS Rusli, H Abimanyu and S Tursiloadi. Quo vadis of citronella oil and its derivatives. LIPI Press, Jakarta, Indonesia, 2019.

Gusmaini and M Syakir. Response of citronella grass on several phosphate levels application at andosol. Indian J. Agr. Res. 2020; 54, 355-60.

AF Sari, W Manguwardoyo and I Sugoro. Degradation of waste and fresh citronella (Cymbopogon nardus L) using in sacco method in rumen fistulated buffalo. In: Proceedings of the Seminar Nasional Teknologi Peternakan dan Veteriner, West Java, Indonesia. 2017, p. 118-24.

F Gabashe, AO Aremu, J. Gruz, JF Finnie and JV Standen. Phytochemical profiles and antioxidant activity of grasses used in south african traditional medicine. Plants 2020; 9, 371.

NA Abdel-Aziz, AZM Salem, MM El-Adawy, LM Camacho, AE Kholif, MMY Elghandour and BE Borhami. Biological treatments as a mean to improve feed utilization in agriculture animals - an overview. J. Integr. Agr. 2015, 14; 534-43.

AT Adesogan, KG Arriola, Y Jiang, A Oyebade, EM Paula, AA Pech-Cervantes, JJ Romero, LF Ferraretto and D Vyas. Symposium review: Technologies for improving fiber utilization. J. Dairy Sci. 2019; 102, 5726-55.

M Wanapat, S Kang, P Khejornsrat and S Wanapat. Effects of plant herb combination supplementation on rumen fermentation and nutrient digestibility in beef cattle. Asian Australas. J. Anim. Sci. 2013; 26, 1127-36.

MF Vázquez-Carrillo, HD Montelongo-Pérez, M González-Ronquillo, E Castillo-Gallegos and OA Castelán-Ortega. Effects of three herbs on methane emissions from beef cattle. Animals 2020; 10, 1671.

Elihasridas, M Zain, RWS Ningrat, Erpomen, M Makmur and EM Putri. Ammonia and fermentation treatment of Cymbopogon nardus L. waste as a substitution of grass: Effect on nutritional profile and ruminal in vitro digestibility. J. Anim. Health Prod. 2021; 9, 27-32.

Riswandi, AIM Ali, S Sandi and Muhakka. Application of ammoniation-fermentation technology based on palm plantation waste for increasing productivity of pampangan buffalo. APCBEE Proc. 2014; 8, 93-8.

A Noersidiq, Y Marlida, M Zain, A Kasim, F Agustin and N Huda. The effect of urea levels on in-vitro digestibility and rumen fermentation characteristic of ammoniated oil palm trunk. Int. J. Adv. Sci. Eng. Inform. Tech. 2020; 10, 1258-62.

Association of Official Analytical Chemists. Official methods of analysis. 18th ed. Association of Official Analytical Chemists, Maryland, 2005.

T Sutardi. Principles of animal nutrition (in Indonesian). Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia, 1980.

HK Goering and PJ Van Soest. Forage fiber analyses (apparatus, reagents, procedures, and some applications). Agricultural Research Service, United States Department of Agriculture, Washington DC, 1970.

JM Tilley and RA Terry. A two-stage technique for in vitro digestion of forage crops. Grass Forage Sci. 1963; 18, 104-11.

EJ Conway and E O’Malley. Microdiffusion methods: Ammonia and urea using buffered absorbents (revised methods for ranges greater than 10 μg. N). Biochem. J. 1942; 36, 655-61.

R Pazla, N Jamarun, M Zain and Arief. Microbial protein synthesis and in vitro fermentability of fermented oil palm fronds by Phanerochaete chrysosporium in combination with tithonia (Tithonia diversifolia) and elephant grass (Pennisetum purpureum). Pakistan J. Nutr. 2018; 17, 462-70.

K Ogimoto and S Imai. Atlas of rumen microbiology. Japan Scientific Societies Press, Tokyo, Japan, 1981.

A Moss, JP Jouany and J Newbold. Methane production by ruminants: Its contribution to global warming. Annales de Zootechnie 2000; 49, 231-53.

MA Goss-Sampson. Statistical analysis in JASP: A guide for students. 2nd ed. Department of Psychological Methods, University of Amsterdam, Amsterdam, The Netherlands, 2019.

R Manurung, R Melinda, MY Abduh, A Widiana, I Sugoro and D Suheryadi. Potential use of lemongrass (Cymbopogon winterianus) residue as dairy cow feed. Pakistan J. Nutr. 2015; 14, 919-23.

M Ebrahimi, MA Rajion, GY Meng, P Shokryzadan, AQ Sazili and MF Jahromi. Feeding oil palm (Elaeis guineensis, Jacq.) fronds alters rumen protozoal population and ruminal fermentation pattern in goats. Ital. J. Anim. Sci. 2015; 14, 403-9.

P Chanjula, V Petcharat and A Cherdthong. Rumen characteristics and feed utilization in goats fed with biologically treated oil palm fronds as roughage in a total mixed ration. S. Afr. J. Anim. Sci. 2018; 48, 1049-56.

R Mukherjee, R Chakraborty and A Dutta. Role of fermentation in improving nutritional quality of soybean meal-a review. Asian Australas. J. Anim. Sci. 2016; 29, 1523-9.

A Fitri, T Obitsu, T Sugino and A Jayanegara. Ensiling of total mixed ration containing persimmon peel: Evaluation of chemical composition and in vitro rumen fermentation profiles. Anim. Sci. J. 2020; 91, e13403.

AP Dewi, M Ridla, EB Laconi and A Jayanegara. Increasing the quality of agricultural and plantation residues using combination of fiber cracking technology and urea for ruminant feeds. Trop. Anim. Sci. J. 2018; 4, 137-46.

S Bureenok, C Yuangklang, K Vasupen, JT Schonewille and Y Kawamoto. The effects of additives in napier grass silages on chemical composition, feed intake, nutrient digestibility and rumen fermentation. Asian Australas. J. Anim. Sci. 2012; 25, 1248-54.

M Wanapat, A Cherdthong, P Pakdee and S Wanapat. Manipulation of rumen ecology by dietary lemongrass (Cymbopogon citratus Stapf.) powder supplementation. J. Anim. Sci. 2008; 86, 3497-503.

RWS Ningrat, M Zain, Erpomen, EM Putri and M Makmur. Effects of Leucaena leucocephala supplementation to total mixed ration based on ammoniated rice straw on fiber digestibility and rumen fermentation characteristics in vitro. Int. J. Adv. Sci. Eng. Inform. Tech. 2019; 9, 916-21.

M Zain, EM Putri, RWS Ningrat, Erpomen and M Makmur. Effects of supplementing Gliricidia sepium on ration based ammoniated rice straw in ruminant feed to decrease methane gas production and to improve nutrient digestibility (in vitro). Int. J. Adv. Sci. Eng. Inform. Tech. 2020; 10, 724-9.

S Chumpawadee, K Sommart, T Vongpralub and V Pattarajinda. In sacco degradation characteristics of protein feed sources in Brahman-Thai native crossbred steers. Walailak J. Sci. Tech. 2005; 2, 219-29.

J Riyanto, E Baliarti, T Hartatik, DT Widayati and LM Yusiati. Evaluation of protein protected in the cow beef cattle rations base-on the fermentation and microbia activities ruments by in vitro. In: Proceedings of the 7th International Seminar on Tropical Animal Production, Yogyakarta, Indonesia. 2017, p. 824-9.

SD Widyawati, WPS Suprayogi, R Utomo and A Ratriyanto. An in vitro study to reduce methane emission in cattle through rumen fermentability modification. AIP Conf. Proceed. 2018; 2014, 020143.

P Mc Donald, RA Edwards, JFD Greenhalgh and CA Moran. Animal Nutrition. 7th ed. Pearson, London, 2010.

L Monzote, O Alarcon and WN Setzer. Antiprotozoal activity of essential oils. Agr. Conspect. Sci. 2012; 77, 167-75.

O Avoseh, O Oyedeji, P Rungqu, B Nkeh-Chungag and A Oyedeji. Cymbopogon species; ethnopharmacology, phytochemistry and the pharmacological importance. Molecules 2015; 20, 7438-53.

RAP Purba, C Yuangklang and P Paengkoum. Enhanced conjugated linoleic acid and biogas production after ruminal fermentation with Piper betle L. supplementation. Ciênc. Rural 2020; 50, e20191001.

RAP Purba, S Paengkoum, C Yuangklang and P Paengkoum. Flavanoids and their aromatic derivatives in Piper betle powder promote in vitro methane mitigation in a variety of diets. Ciênc. Agrotech. 2020; 44, e012420.

RAP Purba, C Yuangklang, S Paengkoum and P Paengkoum. Piper oil decreases in vitro methane production with shifting ruminal fermentation in a variety of diets. Int. J. Agr. Biol. 2021; 25, 231-40.

A Patra, T Park, M Kim and Z Yu. Rumen methanogens and mitigation of methane emission by anti-methanogenic compounds and substances. J. Anim. Sci. Biotechnol. 2017; 8, 13.

P Yuliana, EB Laconi, A Jayanegara, SS Achmadi and AA Samsudin. Effect of napier grass supplemented with Gliricidia sepium, Sapindus rarak or Hibiscus rosa-sinensis on in vitro rumen fermentation profiles and methanogenesis. J. Indonesian Trop. Anim. Agr. 2019; 44, 167-76.

C Suriyapha, T Ampapon, B Viennasay, M Matra, C Wann and M Wanapat. Manipulating rumen fermentation, microbial protein synthesis, and mitigating methane production using bamboo grass pellet in swamp buffaloes. Trop. Anim. Health Prod. 2019; 52, 1609-15.

Samadi, SM Pratama, S Wajizah and A Jayanegara. Evaluation of agro-industrial by products as potential local feed for ruminant animals: volatile fatty acid and NH3 concentration, gas production and methane emission. IOP Conf. Ser. Earth Environ. Sci. 2020; 425, 012010.

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Published

2022-07-05

How to Cite

Elihasridas, E. ., Zain, M. ., Ningrat, R. W. S. ., Erpomen, E. ., Makmur, M. ., Putri, E. M. ., & Halimatuddini, H. . (2022). Evaluation of Pre-ingestive Citronella Residues using Ruminal In Vitro Techniques. Trends in Sciences, 19(14), 5096. https://doi.org/10.48048/tis.2022.5096