Endophytic Fungi from Mangrove Plant Acanthus ilicifolius L.: Antimicrobial, Anticancer, and Species Determination
DOI:
https://doi.org/10.48048/tis.2023.5089Keywords:
MTT assay, Internal transcribed spacer (ITS), Pestalotiopsis sp, Schizopyllum commune, Trichoderma yunnanenseAbstract
The current study aimed to screen, identify and evaluate the biological activity of fungi strains from the mangrove plant Acanthus ilicifolius L, which has proven to be a rich source of fungal species. Thirteen fungi were screened from the leaves, stem bark, and roots. Each fungal strain was cultivated in rice as media, and then the secondary metabolites of the fungus were extracted with ethyl acetate. The extracts were tested for antimicrobial and cytotoxic activities. Next, a phylogenetic tree was reconstructed based on the sequence similarities of each strain. The fungal extract AIA1 displayed excellent antimicrobial activity in a concentration of 5 % against some pathogenic bacterial strains with an inhibition zone in the range of 11.87 ± 0.87 to 15.42 ± 0.51 mm. The brine shrimp lethality test (BSLT) showed that strains AIA1 and AIKB3 were toxic against Artemisia salina L with an LC50 value of less than 80 ppm. The MTT assay determined the cytotoxicity against the human ductal breast epithelial tumor (T47D) cell line. Based on molecular identification, AIA1, AIKB3, and AID1 showed maximum similarity (> 95 %) with Pestalotiopsis sp. AIA1, Trichoderma yunnanense AIKB3, and Schizopyllum commune AID1, respectively. The ethyl acetate extracts of fungi strains from the mangrove plant A. ilicifolius showed strong antibacterial and moderate cytotoxic activities, which make them good candidates for secondary metabolite isolation.
HIGHLIGHTS
- This is the first report on exploring the endophytic fungi from the mangrove plant Acanthus ilicifolius collected from Mangrove Forest Tourism Park, West Sumatera, Indonesia
- Endophytic fungi strains from mangrove plant Acanthus ilicifolius showed good activity on the antimicrobial and cytotoxic assay
- Three potential endophytic fungi were identified asPestalotiopsis AIA1, Trichoderma yunnanense AIKB3, and Schizopyllum commune AID1
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H Thahoi, BC Behera, RR Mishra and SK Dutta. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: A review. Ann. Microbiol. 2013; 63, 1-19.
D Singh and V Aeri. Phytochemical and pharmacological potential of Acanthus ilicifolius. J. Pharm. Bioallied Sci. 2013; 5, 17-20.
HW Zhang, YC Song, and RX Tan. Biology and chemistry of endophytes. Nat. Prod. Rep. 2006; 23, 753-71.
A Debbab, AH Aly and P Proksch. Mangrove-derived fungal endophytes - a chemical and biological perception. Fungal Divers. 2013; 61, 1-27.
BH Babu, BS Shylesh and J Padikkala. Tumour reducing and anticarcinogenic activity of Acanthus ilicifolius in mice. J. Ethnopharmacol. 2002; 79, 27-33.
KTMS Kumar, B Gorain, DK Roy, Zothanpuia, SK Samanta, M Pal, P Biswas, A Roy, D Adhikari, S Karmakar and T Sen. Anti-inflammatory activity of Acanthus ilicifolius. J. Ethnopharmacol. 2008; 120, 7-12.
P Khajure and J Rathod. Antimicrobial activity of extracts of Acanthus ilicifolius extracted from the mangroves of Karwar coast Karnataka. Recent Res. Sci. Tech. 2010; 2, 98-9.
D Handayani, MA Artasasta, D Mutia, N Atikah, Rustini and TE Tallei. Antimicrobial and cytotoxic activities screening of fungal secondary metabolites straind from marine sponge Callyspongia sp. AACL Bioflux 2021; 14, 249-58.
D Handayani, N Sandrawati, R Ruslan, O Nestianda, A Fajrina and TE Tallei. Cytotoxic and antimicrobial activities of ethyl acetate extract of mangrove plant Scyphiphorahydrophyllacea C. F. Gaertn-Associated fungi. J. Appl. Pharmaceut. Sci. 2019; 9, 75-9.
N Sandrawati, R Pariatno, N Suharti and D Handayani. In vitro, cytotoxic activity assay of bacteria extracts derived marine sponge Haliclona fascigera toward Hela, WiDr, T47D, and Vero cell line. J. Appl. Pharmaceut. Sci. 2019; 9, 66-70.
J Kjer, A Debbab, AH Aly and P Proksch. Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nat. Protocol. 2010; 5, 479-90.
D Handayani, W Rasyid, Rustini, EN Zainudin and T Hertiani. Cytotoxic activity screening of fungal extracts derived from the West Sumatran marine sponge Haliclona fascigera to several human cell lines: Hela, WiDr, T47D and Vero. J. Appl. Pharmaceut. Sci. 2018; 8, 55-8.
N Sandrawati, SP Hati, F Yunita, AE Putra, F Ismed, TE Tallei, T Hertiani and D Handayani. Antimicrobial and cytotoxic activities of marine sponge-derived fungal extracts straind from Dactylospongia sp. J. Appl. Pharmaceut. Sci. 2020; 10, 28-33.
Fatimawali, B Kepel and TE Tallei. Potential of organic mercury-resistant bacteria straind from mercury contaminated sites for organic mercury remediation. J. Biol. Sci. 2019; 22, 45-50.
C Ferrer, F Colom, S Frasés, E Mulet, JL Abad and JL Alió. Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J. Clin. Microbiol. 2001; 39, 2873-9.
S Kumar, G Stecher and K Tamura. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 2016; 33, 1870-4.
WW Davis and TR Stout. Disc plate method of microbiological antibiotic assay. II. Novel procedure offering improved accuracy. Appl. Microbiol. 1971; 22, 666-70.
BN Meyer, RN Ferrign, JE Putnam, JE Jacobson, DE Nicholas and JL Mclaughlin. Brineshrimp: A convenient general bioassay for active plant constituents. Planta Med. 1982; 45, 31-4.
M Qiao, X Du, Z Zhang, JP Xu and ZF Yu. Three new species of soil-inhabiting trichoderma from southwest China. MycoKeys 2018; 44, 63-80.
LÁ Castro, MI Álvarez and E Martínez. Case report of schizophyllumcommunesinusitis in an immunocompetent patient. Colombia Med. 2010; 41, 71-5.
S Chandra. Endophytic fungi: Novel sources of anticancer lead molecules. Appl. Microbiol. Biotechnol. 2012; 95, 47-59.
J Rokach, Y Girard and JG Atkinson. ChemInform Abstract: first successful substitution of 5H-dibenzo(A,D)cyclohepten-5-one. Chemischer Informationsdienst 1980, https://doi.org/10.1002/chin.198008220.
PK Minocha and KP Tiwari. A triterpenoidal saponin from roots of Acanthus illicifolius. Phytochemistry 1981; 20, 135-7.
PV Kiem, TH Quang, TT Huong, LTH Nhung, NX Cuong, CV Minh, EM Choi and YH Kim. Chemical constituents of Acanthus ilicifolius L. and effect on osteoblastic MC3T3E1 cells. Arch. Pharm. Res. 2008; 31, 823-9.
C Wei-Chiung, W Chen, H Chih-Chiao, G Sheng-Yu, C Hyo-Jung, LM Tsang, TW Ho and P Ka-Lai. A highly diverse fungal community associated with leaves of the mangrove plant Acanthus ilicifolius var. xiamenensis revealed by isolation and metabarcoding analyses. PeerJ 2019; 7, e7293.
B Zhi-Qiang, X Lin, Y Wang, J Wang, X Zhou, B Yang, J Liu, X Yang, Y Wang and Y Liu. New phenyl derivatives from endophytic fungus Aspergillus flavipes AIL8 derived of mangrove plant Acanthus ilicifolius. Fitoterapia 2014; 95, 194-202.
K Tarman, U Lindequist, K Wende, A Porzel, N Arnold and LA Wessjohann. Isolation of a new natural product and cytotoxic and antimicrobial activities of extracts from fungi of Indonesian marine habitats. Mar. Drugs 2011; 9, 294-306.
VK Mishra, AK Passari, P Chandra, VV Leo, B Kumar, S Uthandi, S Thankappan, VK Gupta and BP Singh. Determination and production of antimicrobial compounds by Aspergillus clavatonanicus strain MJ31, an endophytic fungus from Mirabilis jalapa L. using UPLC-ESI-MS/MS and TD-GC-MS analysis. PLos One 2017; 12, 0186234.
K Bockstael and AV Aerschot. Antimicrobial resistance in bacteria. Cent. Eur. J. Med. 2009; 4, 141-55.
GL Maria, KR Sridhar and NS Raviraja. Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India. J. Agr. Tech. 2005; 1, 67-80.
C Wei-Chiung, P Ka-Lai, C Wei-Ling, W Guei-Jane and L Tzong-Huei. Antimicrobial and iNOS inhibitory activities of the endophytic fungi straind from the mangrove plant Acanthus ilicifolius var. xiamenensis. Bot. Stud. 2019; 60, 4.
J Xu, AH Aly, V Wray and P Proksch. Chromones from the endophytic fungus Pestalotiopsis sp. straind from the Chinese mangrove plant Rhizophora mucronata. Tetrahedron Lett. 2011; 52, 21-5.
J Wang, H Zhu, Z Hu and Y Zhang. Cytotoxic butenolides and diphenyl ethers from the endophytic fungus Pestalotiopsis sp. Phytochemistry Lett. 2019; 29, 186-9.
RY Song, XB Wang, GP Yin, RH Liu, LY Kong and MH Yang. Isocoumarin derivatives from the endophytic fungus, Pestalotiopsis sp. Fitoterapia 2017; 122, 115-8.
C Yuan, G Ding, W Hai-Ying, G Yu-Hua, H Shang, M Xiao-Jun and Z Zhong-Mei. Polyketide-terpene hybrid metabolites from an endolichenic fungus Pestalotiopsis sp. Biomed Res. Int. 2017; 2017, 6961928.
YB Zheng, DX Chen, JJ Li and JZ Huang. New sesquiterpenoid from Pestalotiopsis sp. Chin. J. Nat. Med. 2011; 9, 421-4.
KD Beattie, N Ellwood, R Kumar, X Yang, PC Healy, V Choomuenwai, RJ Quinn, AG Elliott, JX Huang, JL Chitty, JA Fraser, MA Cooper and RA Davis. Antibacterial and antifungal screening of natural products sourced from Australian fungi and characterisation of pestalactams D-F. Phytochemistry 2016; 124, 79-85.
IH Hwang, DT Wicklow and JB Gloer. New punctaporonins from two fungicolous strains of Pestalotiopsis sp. Phytochemistry Lett. 2016; 16, 257-62.
Y Liu, MH Yang, XB Wang, TX Li and LY Kong. Caryophyllene sesquiterpenoids from the endophytic fungus, Pestalotiopsis sp. Fitoterapia 2016; 109, 119-24.
A Tapwal. Antimycotic activity and phytochemical screenng of fungal endophytes associated with Santalum album. Nusantara Biosci. 2016; 8, 14-7.
K Sowparthani. In-vitro phytochemical analysis, high-performance liquid chromatography, and antibacterial activity of endophytic fungi Pestalotiopsis sp. straind from Acalypha indica (Linn). Asian J. Pharmaceut. Clin. Res. 2016; 9, 101-3
JK Harper, DH Barich, JZ Hu, GA Strobel and DM Grant. Stereochemical analysis by solid-state NMR: Structural predictions in ambuic acid. J. Org. Chem. 2003; 68, 4609-14.
GA Strobel. Rainforest endophytes and bioactive products. Crit. Rev. Biotechnol. 2002; 22, 315-33.
K Saravanakumar, R Vivek, NS Boopathy, L Yaqian, K Kathiresan and J Chen. Anticancer potential of bioactive 16-methylheptadecanoic acid methyl ester derived from marine tricoderma. J. Appl. Biomed. 2015; 13, 199-12.
GC Jayakumar, SV Kanth, B Chandrasekaran, JR Rao and BU Nair. Preparation and antimicrobial activity of scleraldehyde from Schizophyllum commune. Carbohydr. Res. 2010; 345, 2213-9.
K Zhong, L Tong, L Liu, X Zhous, X Liu, Q Zhang and S Zhou. Immunoregulatory and antitumor activity of schizophyllan under ultrasonic treatment. Int. J. Biol. Macromol. 2015; 80, 302-8.
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