Variability of Morphological and Agronomical Characteristics of Centella asiatica in Thailand
DOI:
https://doi.org/10.48048/tis.2021.502Keywords:
Asiatic pennywort, Morphology, Diversity, Correlation, PhenotypeAbstract
The phenotypic variability of 30 accessions of Asiatic pennywort (Centella asiatica (L.) Urb.) were collected in different regions in Thailand were evaluated. The growth habit among accessions were identified into 2 types including with 20 accessions of erect from and 10 accessions of flatten from. The leaf arrangement divided into 2 types, including with rosette (20 accessions) and spreading leaf (10 accessions). The variability of morphological and agronomical characteristics experiment was derived by Complete Randomized Design (CRD) with 5 replications. The 11 morphological and agronomical character data were collected at 2 mounts after transplant. The phylogenetic tree manifesting the diversity among 30 accessions based on Euclidean distance coefficient identified by 11 morphological and agronomical characteristics. Analysis of variance revealed high and highly significant difference among accessions for all characteristics, shown a wide range of morphological and agronomical characteristics variability of the Asiatic pennywort germplasm. The phylogenetic tree was classified Asiatic pennywort accessions into 2 different clusters with the distance coefficient ranged from 1.00 - 25.00. The 1st cluster consisted of 8 accessions and the 2nd cluster consisted of 22 accessions. The 2nd cluster was divided into 2 sub- clusters, including with sub-cluster 2.1 consisted of 21 accessions and sub-cluster 2.2 consisted of 1 accession. Mean value of the 2nd cluster was higher than the 1st group all characters except SPAD Index. The result suggested that could be used accession members in the 2nd cluster can be used for future breeding program of Asiatic pennywort in Thailand.
HIGHLIGHTS
- The morphology and actives ingredient components of Asiatic pennywort were depended on the genetic variability and geographical conditions
- The variability based on morphological and agronomic characteristics of Asiatic pennywort was useful for breeding perspective
- The Asiatic pennywort accessions in cluster 2 could be used for breeding program in the future
GRAPHICAL ABSTRACT
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K Padmalatha and MNV Prasad. Genetic diversity in Centella asiatica (L.) Urb., a memory-enhancing neutraceutical herb, using RAPD markers. Med. Aromat. Plant Sci. Biotechnol. 2008; 2, 90-5.
P Yasurin, M Sriariyanun and T Phusantisampan. Review: The bioavailability activity of Centella asiatica. KMUTNB Int. J. Appl. Sci. Tech. 2016; 9, 1-9.
P Hashim. Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int. Food Res. J. 2011; 18, 1215-22.
European Medicines Agency. Assessment report on Centella asiatica (L.) Urban, herba. United Kingdom, 2010, p. 1-44.
NE Gray, AA Magana, P Lak, KM Wright, J Quinn, JF Stevens, CS Maier and A Soumyanath. Centella asiatica – phytochemistry and mechanisms of neuroprotection and cognitive enhancement. Phytochem Rev. 2018; 17, 161-94
P Puttarak, P Dilokthornsakul, S Saokaew, T Dhippayom, C Kongkaew, R Sruamsiri, A Chuthaputti and N Chaiyakunapruk. Effects of Centella asiatica (L.) urb. on cognitive function and mood related outcomes: A systematic review and meta-analysis. Sci. Rep. 2017; 7, 10646.
IE Orhan. Centella asiatica (L.) urban: From traditional medicine to modern medicine with neuroprotective potential. Evid.based Complement. Altern. Med. 2012; 2012, 946259.
R Hamidpour, S Hamidpour, M Hamidpour, M Zarabi, M Sohraby and R Hamidpour. Medicinal property of Gotu kola (Centella asiatica) from the selection of traditional applications to the novel phytotherapy. Arch. Cancer Res. 2015; 3, 1-7.
MV Sudhakaran. Botanical pharmacognosy of Centella asiatica (Linn.) urban. Pharmacogn. J. 2017; 9, 546-58.
S Srivastava, S Tiwari, N Srivastava, S Verma and AKS Rawat. Chemotaxonomic studies on Centella asiatica (L.) urb. from varied phytogeographical conditions of India for its industrial prospection. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 2019; 89, 1057-66.
A Prasad, SS Dhawan, AK Mathur, O Prakash, MM Gupta, RK Verma, RK Lal and A Mathur. Morphological, chemical and molecular characterization of Centella asiatica germplasms for commercial cultivation in the Indo-Gangetic plains. Nat. Prod. Commun. 2014; 9, 779-84.
J Srithongkul, S Kanlayanarat, V Srilaong, A Uthairatanakij and P Chalermglin. Effects of light intensity on growth and accumulation of triterpenoids in three accessions of Asiatic pennywort (Centella asiatica (L.) urb.). J. Food Agric. Environ. 2011; 9, 360-3.
XG Zhang, T Han, ZG He, QY Zhang, L Zhang, K Rahman and LP Qin. Genetic diversity of Centella asiatica in China analyzed by inter-simple sequence repeat (ISSR) markers: Combination analysis with chemical diversity. J. Nat. Med. 2012; 66, 241-7.
BW Gbolahan, AI Abiola, J Kamaldin, MA Ahmad and MS Atanassova. Accession in Centella asiatica; current understanding and future knowledge. J. Pure Appl. Microbio. 2016; 10, 2485-94.
HM Mumtazah, Supriyono, Y Widyastuti and A Yunus. The diversity of leaves and asiaticoside content on three accessions of Centella asiatica with the addition of chicken manure fertilizer. Biodiversitas. 2020; 21, 1035-40.
A Alqahtani, JL Cho, KH Wong, KM Li, V Razmovski-Naumovski and GQ Li. Differentiation of three Centella species in Australia as inferred from morphological characteristics, ISSR molecular fingerprinting and phytochemical composition. Front. Plant Sci. 2017; 8, 1980.
CHWMRB Chandrasekara, RAPI Sumanarathne and PCG Bandaranayake. Centellaasiatica morphotypes differ genetically as well as macronutrients content, total phenolic content and chemical fingerprints of leaves. J. Agric. Sci. Sri Lanka. 2020; 15, 75.
M Sakthipriya, SS Vishnu, S Sujith, PR Kumar and KK Sabu. Analysis of genetic diversity of Centella asiatica using SSR markers. Int. J. Appl. Sci. Biotechnol. 2018; 6, 103-9.
P Puttarak and P Panichayupakaranant. Factors affecting the content of pentacyclic triterpenes in Centella asiatica raw materials. Pharm. Biol. 2012; 50, 1508-12.
F Zavinon, H Adoukonou-Sagbadja, A Bossikponnon, H Dossa and C Ahanhanzo. Phenotypic diversity for agro-morphological traits in pigeon pea landraces (Cajanus cajan L.) millsp. cultivated in southern Benin. Open Agric. 2019; 4, 487-99.
NL Hien, WA Sarhadi, Y Oikawa and Y Hirata. Genetic diversity of morphological responses and the relationships among Asia aromatic rice (Oryza sativa L.) cultivars. Tropics 2007; 16, 343-55.
S Kachare, S Tiwari, N Tripathi and VV Thakur. Assessment of genetic diversity of soybean (Glycine max) genotypes using qualitative traits and microsatellite markers. Agr. Res. 2020; 9, 23-34.
CS Ravi1, K Umesha, K HimaBindu, GR Shetty and GSA Kumar. Collection and morphological variability in ecotypes of Indian pennywort (Centella asiatica L.) of hill zone of Karnataka, India. Int. J. Curr. Microbiol. App. Sci. 2019; 8, 994-1008.
SOR Rakotondralambo, M Rodier-Goud, R Rivallan, A Lussert, P Danthu, F Lamotte, E Ralambofetra, P Ramavovololona, JL Noyer and FC Baurens. Insight into the biology, genetics and evolution of the Centella asiatica polyploidy complex in Madagascar. Ind. Crop Prod. 2013; 47, 118-25.
G Chomicki, YM Staedler, LPR Bidel, C Jay-Allemand, J Schönenberger and SS Renner. Deciphering the complex architecture of an herb using micro-computed X-ray tomography, with an illustrated discussion on architectural diversity of herbs. Bot. J. Linn. Soc. 2018; 186, 145-57.
ZA Aziz, MR Davey, JB Power, P Anthony, RM Smith and KC Lowe. Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo. Biol. Plant. 2007; 51, 34-42.
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