Evaluation an Aqueous Extract of Irradiated and Non Irradiated of Orthosiphon Aristatus on Zebrafish Embryo (Danio Rerio) through Acute Toxicity Assay
DOI:
https://doi.org/10.48048/tis.2022.4980Keywords:
Orthosiphon aristatus, Irradiation, Zebrafish embryo, Heartbeat, HatchingAbstract
Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irradiated and irradiated Orthosiphon aristatus at different dosage of 3, 6, 9 and 12 kilogray (kGy) on zebrafish embryo. Method: Survival rate, hatching rate, heart beat rate and scoliosis were observed and date were analysed using SPSS 25.00 windows. Result: The lethal dose (LC50) of non-irradiated Orthosiphon aristatus is 381.81 μg/mL compare to irradiated extract at 3 % is (371.27 μg/mL), 6 % (311.03 μg/mL), 9 % (160.72 μg/mL) and 12 % (140.18 μg/mL). Hatchability of zebrafish embryo for Orthosiphon aristatus extract decrease in the higher dosage of irradiated sample compared to non-irradiated sample. No presence of scoliosis was observed in all dosage of irradiated and non-irradiated of Orthosiphon aristatus. The heartbeat of zebrafish embryo treated with irradiated Orthosiphon aristatus specifically 12 % show decrease at 250 μg/mL concentration. Remaining dosage of irradiated and non-irradiated Orthosiphon aristatus show average heartbeat around 120 - 160 bpm. Conclusion: As conclusion, irradiated and non-irradiated of Orthosiphon aristatus is safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo.
HIGHLIGHTS
- Herbal medicine (Orthosiphon aristatus) is widely used for treatment as alternative medicine
- This Orthosiphon aristatus easy can be contaminated by bacteria and fungus. Gamma ray is helpful to eliminated the bacteria and fungus
- Irradiated Orthosiphon aristatus can be toxic due to the exposure in Gamma ray
- Toxicity study using zebra fish show no changes in toxicity of Orthosiphon aristatus
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