Antifungal Activity of Streptomyces spectabilis SP-O2 against Aflatoxin Producing Mold, Aspergillus flavus
DOI:
https://doi.org/10.48048/tis.2022.375Keywords:
Streptomyces spectabilis, Aspergillus flavus, Aflatoxin, Antifungal activity, BiocontrolAbstract
Among soil isolated species of actinomycetes bacteria that affected the growth of aflatoxin producing mold; Aspergillus flavus TISTR 3041, the isolate SP-O2 showed a promising inhibitory activity on the dual culture assay. This isolated bacterium was characterized on various International Streptomyces Project media. The macroscopic morphological analysis showed mycelium, sporogenesis, and orangish pink of colonial pigment. The scanning electron microscope (SEM) photograph showed that the sporogenesis has occurred by shrinking of both sides of aerial mycelium. After the sporogenesis has been completed, the fragmentation of altered mycelium started from the tips of aerial mycelium to produce 0.8-1 micrometer fragmented spores. The 16S rRNA sequences analysis revealed that isolate SP-O2 shared a 99.93 % (1 mismatch/1436 nt) similarity with Streptomyces spectabilis NBRC 13424 AB184393. Co-culture liquid assay was analyzed for the aflatoxin produced by A. flavus in the presence of Streptomyces spectabilis SP-O2. The result showed that UV-visualized band of aflatoxin B1 was not detected by co-cultivation of Streptomyces spectabilis SP-O2 with A. flavus by thin layer chromatography compared to the control. Indeed, fungal growth of A. flavus was destroyed by the antifungal substance produced by Streptomyces spectabilis SP-O2 in the liquid culture. Furthermore, biocontrol of contaminated A. flavus was conducted on peanut kernels and the results showed that Streptomyces spectabilis SP-O2 has antifungal activity against the presence of A. flavus. Together, these results suggested that further investigation on the development of biocontrol use of Streptomyces spectabilis SP-O2 to overcome the contamination of aflatoxin producing mold in the feedstock and agricultural-based industry is needed.
HIGHLIGHTS
- The isolated actinobacteria in this study was identified as Streptomyces spectabilis isolate SP-O2 based on its morphological characteristics, physiological properties and molecular identification
- SEM showed the sporogenesis of 0.8-1 micrometer fragmented spores
- Streptomyces spectabilis isolate SP-O2 exhibited the promising fungicidal activity against flavus in the dual culture agar assay, co-culture liquid assay and peanut kernels assay with consistency results
- The secreted metabolites play a crucial role on the fungal killing against Aspergillus flavus rather than the aflatoxin degradation
GRAPHICAL ABSTRACT
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HPV Egmond, RC Schothorst and MA Jonker. Regulations relating to mycotoxins in food: Perspectives in a global and European context. Anal. Bioanal. Chem. 2007; 389, 147-57.
GA Payne and MP Brown.Genetics and physiology of aflatoxin biosynthesis. Annu. Rev. Phytopathol. 1998; 36, 329-62.
CP Wild and YY Gong. Mycotoxins and human disease: A largely ignored global health issue. Carcinogenesis 2010; 31, 71-82.
M Carvajal-Moreno. Metabolic changes of aflatoxin B1 to become an active carcinogen and the control of this toxin. Immunome Res. 2015; 11, 104.
IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monogr.Eval.Carcinog.Risks Hum. 2002; 82, 1-556.
G Perrone, A Susca, G Cozzi, K Ehrlich, J Varga, JC Frisvad, M Meijer, P Noonim, W Mahakarnchanakul and RA Samson. Biodiversity of Aspergillus species in some important agricultural products. Stud. Mycol. 2007; 59, 53-66.
EA Emmert and J Handelsman. Biocontrol of plant disease: A (gram-) positive perspective. FEMS Microbiol. Lett. 1999; 171, 1-9.
T Kaur, R Rani and RK Manhas.Biocontrol and plant growth promoting potential of phylogenetically new Streptomyces sp. MR14 of rhizospheric origin. AMB Express2019; 9, 125.
PE Verweij, E Snelders, GHJ Kema, E Mellado and WJG Melchers. Azole resistance in Aspergillus fumigatus: A side-effect of environmental fungicide use? Lancet Infect. Dis. 2009; 9, 789-95.
SN Kumar, B Nambisan and C Mohandas. Purification and identification of two antifungal cyclic dipeptides from Bacillus cereus subsp. thuringiensis associated with a rhabditid entomopathogenic nematode especially against Fusarium oxysporum. J. Enzyme Inhib. Med. Chem. 2014; 29, 190-7.
SD Bentley, KF Chater, AM Cerdeño-Tárraga, GL Challis, NR Thomson, KD James, DE Harris, MA Quail, H Kieser, D Harper, A Bateman, S Brown, G Chandra, CW Chen, M Collins, A Cronin, A Fraser, A Goble, J Hidalgo, T Hornsby, … and DA Hopwood. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 2002; 417, 141-7.
J Abraham and R Chauhan. Profiling of red pigment produced by Streptomyces sp. JAR6 and its bioactivity. 3 Biotech2018; 8, 22.
I Caceres, SP Snini, O Puel and F Mathieu. Streptomyces roseolus, a promising biocontrol agent against Aspergillus flavus, the main aflatoxin B₁ producer. Toxins2018; 10, 442.
HM Kemung, LTH Tan, TM Khan, KG Chan, P Pusparajah, BH Gohand LH Lee. Streptomyces as a prominent resource of future anti-MRSA drugs. Front. Microbiol. 2018; 9, 2221.
D Qi, L Zou, D Zhou, Y Chen, Z Gao, R Feng, M Zhang, K Li, J Xieand W Wang. Taxonomy and broad-spectrum antifungal activity of Streptomyces sp. SCA3-4 isolated from rhizosphere soil of Opuntia stricta. Front. Microbiol. 2019; 10, 1390.
JN Selvakumar, SD Chandrasekaran and M Vaithilingam. Bio prospecting of marine-derived Streptomycesspectabilis VITJS10 and exploring its cytotoxicity against human liver cancer cell lines. Pharmacogn. Mag. 2015; 11, S469-S473.
Q Shakeel, A Lyu, J Zhang, M Wu, G Li, T Hsiang and L Yang. Biocontrol of Aspergillus flavus on peanut kernels using Streptomyces yanglinensis 3-10. Front. Microbiol. 2018; 9, 1049.
B Zhang, G Cai, H Wang, D Li, X Yang, X An, X Zheng, Y Tian, W Zheng and T Zheng. Streptomyces alboflavus RPS and its novel and high algicidal activity against harmful algal bloom species Phaeocystisglobosa. PLoS One 2014; 9, e92907.
S Boukaew and P Prasertsan. Efficacy of volatile compounds from Streptomyces philanthi RL-1-178 as a biofumigant for controlling growth and aflatoxin production of the two aflatoxin-producing fungi on stored soybean seeds. J. Appl. Microbiol. 2020; 129, 652-64.
M Yang, L Lu, J Pang, Y Hu, Q Guo, Z Li, S Wu, H Liu and C Wang. Biocontrol activity of volatile organic compounds from Streptomyces alboflavus TD-1 against Aspergillus flavus growth and aflatoxin production. J. Microbiol. 2019; 57, 396-404.
A Janardhan, AP Kumar, B Viswanath, DVR Saigopal and G Narasimha. Production of bioactive compounds by actinomycetes and their antioxidant properties. Biotechnol. Res. Int. 2014; 2014, 217030.
P Sangmanee and T Hongpattarakere. Inhibitory of multiple antifungal components produced by Lactobacillus plantarum K35 on growth, aflatoxin production and ultrastructure alterations of Aspergillus flavus and Aspergillus parasiticus. Food Control 2014; 40, 224-33.
SK Augustine, SP Bhavsar, M Baserisalehi and BP Kapadnis. Isolation, characterization and optimization of antifungal activity of an actinomycete of soil origin. Indian J. Exp. Biol. 2004; 42, 928-32.
EB Shirling and D Gottlieb. Methods for characterization of Streptomyces species. Int. J. Syst.Evol. Microbiol. 1966; 16, 313-40.
MC Taylor, CJ Jackson, DB Tattersall, N French, TS Peat, J Newman, LJ Briggs, GV Lapalikar, PM Campbell, C Scott, RJ Russell and JG Oakeshott. Identification and characterization of two families of F420 H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Mol. Microbiol. 2010; 78, 561-75.
SF Altschul, TL Madden, AA Schäffer, J Zhang, Z Zhang, W Miller and DJ Lipman. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 1997; 25, 3389-402.
EW Myers and W Miller. Optimal alignments in linear space. Comput. Appl. Biosci. 1988; 4, 11-7.
S Kumar, G Stecher, M Li, C Knyaz and K Tamura. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 2018; 35, 1547-9.
P Kumar, DK Mahato, M Kamle, TK Mohanta and SG Kang. Aflatoxins: A global concern for food safety, human health and their management. Front. Microbiol. 2016; 7, 2170.
K Kurosawa, K Takahashi and E Tsuda. SNF4435C and D, novel immunosuppressants produced by a strain of Streptomyces spectabilis. I. Taxonomy, fermentation, isolation and biological activities. J. Antibiot. 2001; 54, 541-7.
S Guan, C Ji, T Zhou, J Li, Q Ma and T Niu. Aflatoxin B1 degradation by Stenotrophomonas maltophilia and other microbes selected using coumarin medium. Int. J. Mol. Sci. 2008; 9, 1489-503.
OD Teniola, PA Addo, IM Brost, P Färber, KD Jany, JF Alberts, WHV Zyl, PS Steyn and WH Holzapfel. Degradation of aflatoxin B(1) by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556(T). Int. J. Food Microbiol. 2005; 105, 111-7.
S Alinezhad, M Tolouee, A Kamalzadeh, AA Motalebi, M Nazeri, M Yasemi, M Shams-Ghahfarokhi, R Tolouei and M Razzaghi-Abyaneh. Mycobiota and aflatoxin B1 contamination of rainbow trout (Oncorhinchus mykiss) feed with emphasis to Aspergillus section Flavi. Iranian J. Fish. 2011; 10, 363-74.
KR Kim, TJ Kim and JW Suh. The gene cluster for spectinomycin biosynthesis and the aminoglycoside-resistance function of spcM in Streptomyces spectabilis. Curr. Microbiol. 2008; 57, 371-4.
D Spasova, N Vesselinova and R Gesheva. Comparative investigation of a streptovaricin-producing strain of Streptomyces spectabilis and its selectant. Folia Microbiol. 1997; 42, 35-8.
Y Chen, D Zhou, D Qi, Z Gao, J Xie and Y Luo. Growth promotion and disease suppression ability of a Streptomyces sp. CB-75 from banana rhizosphere soil. Front. Microbiol. 2017; 8, 2704.
EB Shirling and D Gottlieb. Cooperative description of type strains of Streptomyces. V. additional descriptions. Int. J. Syst. Bacteriol. 1972; 22, 265-394.
M Kataoka, K Ueda, T Kudo, T Seki and T Yoshida. Application of the variable region in 16S rDNA to create an index for rapid species identification in the genus Streptomyces. FEMS Microbiol. Lett. 1997; 151, 249-55.
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