Fabrication of Sponges from Amnion Hydrogel to Apply as Wound Dressing
DOI:
https://doi.org/10.48048/tis.2021.36Keywords:
Amnion hydrogel, Wound dressing, Freeze-drying, Glutaraldehyde, SpongeAbstract
A burn is a type of injury to the skin or other tissues. It can give rise to skin defects or even death. In this study, sponges derived from amnion hydrogel were generated to apply as a wound dressing. The sponges were created by combining crosslinking and freeze-drying methods. There were three types of the obtained sponges: MGA-0 (only washed with PBS), MGA-1 (washed in glycine 1 % for one day) and MGA-2 (washed in glycine 1 % for two days). These sponges were evaluated by scanning electron microscope (SEM), mechanical test, swelling, and cytotoxicity. The tensile strength of the sponges was about 1.8 MPa, and the absorption increased during 24 h. The relative growth rates (%RGR) of MGA-0, MGA-1, and MGA-2 were 88.8, 58.2 and 67.2 %, respectively. The obtained results suggested that the MGA-0 sponge has potential for wound dressing application.
HIGHLIGHTS
- The sponges have been manufactured from human amnion hydrogel by combining crosslinking and freeze-drying methods
- Through the scanning electron microscope (SEM), the surface of the obtained sponges showed a fibrous-like structure
- These sponges have good absorbency, the tensile strength of the sponges is about 1.8 MPa like the tensile strength of human skin, and the MGA-0 sponge is not cytotoxic
GRAPHICAL ABSTRACT
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J Alipour, Y Mehdipour and A Karimi. Epidemiology and outcome analysis of 3030 burn patients with an ICD-10 approach. Ann. Burns Fire Disasters 2020; 33, 3-13.
C Malhotra and AK Jain. Human amniotic membrane transplantation: Different modalities of its use in ophthalmology. World J. Transplant. 2014; 4, 111-21.
K Dhaliwal and N Lopez. Hydrogel dressings and their application in burn wound care. Br. J. Community Nurs. 2018; 23, S24-S27.
A Clement and G Pins. Engineering the tissue-wound interface: Harnessing topography to direct wound healing. Wound Healing Biomater. 2016; 1, 253-75.
SV Murphy, A Skardal, RAJ Nelson, K Sunnon, T Reid, C Clouse, ND Kock, J Jackson, S Soker and A Atala. Amnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model. Stem Cells Transl. Med. 2020; 9, 80-92.
M Tenehaus and HO Rennekampff. Topical agents and dressings for local burn wound care. UpToDate, 2018.
S Sharma, A Dua and A Malik. Third generation materials for wound dressings. Int. J. Pharmaceut. Sci. Res. 2014; 5, 2113-24.
EA Kamoun, ERS Kenawy and X Chen. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J. Adv. Res. 2017; 8, 217-33.
D Queena, JH Evansa, JDS Gaylora, JM Courtneya and WH Reid. Burn wound dressings - a review. Burns 1987; 13, 218-28.
S Dhivya, VV Padma and E Santhini. Wound dressings - a review. BioMedicine (Taipei) 2015; 5, 22.
M Madaghiele, C Demitri, A Sannino and L Ambrosio. Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates. Burns Trauma 2014; 2, 153-61.
C Weller and V Team. Interactive dressings and their role in moist wound management. In: S Rajendran (Eds.). Advanced textiles for wound care. 2nd ed. Woodhead Publishing Limited, Sawston, 2019, p. 105-34.
S Tavakoli and AS Klar. Advanced hydrogels as wound dressings. Biomolecules 2020; 10, 1169.
AC Mamede, MJ Carvalho, AM Abrantes, M Laranjo, CJ Maia and MF Botelho. Amniotic membrane: From structure and functions to clinical applications. Cell Tissue Res. 2012; 349, 447-58.
S Parry and JF Strauss 3rd. Premature rupture of the fetal membranes. N. Engl. J. Med. 1998; 338, 663-70.
SV Murphy, A Skardal, RAJ Nelson, K Sunnon, T Reid, C Clouse, ND Kock, J Jackson and S Soker. A Atala. Amnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model. Stem cells Transl. Med. 2020; 9, 80-92.
DE Fetterolf and RJ Snyder. Scientific and clinical support for the use of dehydrated amniotic membrane in wound management. Wounds 2012; 24, 299-307.
J Oba, M Okabe, T Yoshida, C Soko, M Fathy, K Amano, D Kobashi, M Wakasugi and H Okudera. Hyperdry human amniotic membrane application as a wound dressing for a full-thickness skin excision after a third-degree burn injury. Burns Trauma 2020; 8, 014.
S Leal-Marin, T Kern, N Hofmann, O Pogozhykh, C Framme, M Borgel, C Figueiredo, B Glasmacher and O Gryshkov. Human amniotic membrane: A review on tissue engineering, application, and storage. J. Biomed. Mater. Res. B Appl. Biomater. 2020; 109, 1198-215.
SV Murphy, A Skardal, L Song, K Sutton, R Haug, DL Mack, J Jackson, S Soker and A Atala. Solubilized amnion membrane hyaluronic acid hydrogel accelerates full-thickness wound healing. Stem Cells Transl. Med. 2017; 6, 2020-32.
Q Yao, YW Zheng, HL Lin, QH Lan, ZW Huang, LF Wang, R Chen, J Xiao, L Kou, HL Xu and YZ Zhao. Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction. Artif. Cell. Nanomed. Biotechnol. 2020; 48, 218-29.
MT Wolf, KA Daly, EP Brennan-Pierce, SA Johnson, CA Carruthers, A D'Amore, SP Nagarkar, SS Velankar and SF Badylak. A hydrogel derived from decellularized dermal extracellular matrix. Biomaterials 2012; 33, 7028-38.
I Migneault, C Dartiguenave, MJ Bertrand and KC Waldron. Glutaraldehyde: Behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking. Biotechniques 2004; 37, 790-802.
LHHO Damink, PJ Dijkstra, MJAV Luyn, PBV Wachem, P Nieuwenhuis and J Feijen. Glutaraldehyde as a crosslinking agent for collagen-based biomaterials. J. Mater. Sci. Mater. Med. 1995; 6, 460-72.
LM Delgado, K Fuller and DI Zeugolis. Collagen cross-linking: Biophysical, biochemical, and biological response analysis. Tissue Eng. A 2017; 23, 1064-77.
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