Trends in Sciences

Secretome of Hypoxia-Preconditioned Mesenchymal Stem Cells Ameliorates Hyperglycemia in Type 2 Diabetes Mellitus Rats

Wita Widyaningsih — 2024-03-01

Introduction: Type 2 diabetes mellitus (T2DM) is a prevalent form of diabetes that affects 90 - 95 % of all diabetic patients. Insulin sensitizers and insulin exogenous supply could temporarily ameliorate hyperglycaemia; however, they are accompanied by side effects. As a result, new approaches are required to address insulin resistance and regenerate beta cells simultaneously. The secretome of hypoxic mesenchymal stem cells (SH-MSCs) contains various growth factors and anti-inflammatory cytokines that could potentially enhance insulin resistance and improve pancreatic function. Objectives: In this study, we performed SH-MSCs infusion to ameliorate HFD-induced hyperglycaemia in T2DM rats. Methods: We created a T2DM rat model using a combination of a high-fat diet (HFD) and streptozotocin (STZ) administration. Then, we administered SH-MSCs injection at doses of 250 and 500 µL and assessed the therapeutic effects of SH-MSCs. We also investigated the potential underlying mechanisms involved. Results: The administration of SH-MSCs improved hyperglycemia in rats with T2DM. Infusion of SH-MSCs at 500 µL dose decreased homeostatic model assessment for insulin resistance (HOMA-IR). Histological analysis revealed that injection of SH-MSCs alleviated morphological damage of pancreas. SH-MSCs administration also inhibit the level of IL-6 and promote the expression of CD163 type 2 macrophage. Conclusion: The results of our study indicate that SH-MSCs have the potential to improve hyperglycemia and exert a protective effect on T2DM rats.

HIGHLIGHTS

Administration of SH-MSCs effectively improved hyperglycemia and decreased insulin resistance in TD2M rats through modulation of IL-6 levels and promotes the expression of CD163 type 2 macrophage
Histological analysis demonstrated the protective effect of SH-MSCs on pancreatic morphology
SH-MSCs hold promise for improving hyperglycemia, insulin resistance, and providing a protective effect in TD2M

GRAPHICAL ABSTRACT

The Potential of Purple Sweet Potato-based Food Bar as an Immunomodulator and Hepatoprotector Against Diethylnitrosamine-induced Mice

Tri Dewanti Widyaningsih — 2024-03-10

The food bar’s effect as an immunomodulator and hepatoprotector in vivo was tested on male BALB/c mice induced with diethylnitrosamine (DEN). The experimental animals were treated using the Post-test Control Group Design method. Six groups of 30 mice each made up the sample: The negative control group of healthy mice fed with a normal diet (P0); the group of healthy mice given the food bar (P1); the positive control group of mice induced with DEN and given a normal diet (P2); the group of mice induced with DEN and given a normal diet + food bar at 11,700 mg/kg (P3); the group of mice induced with DEN and given a normal diet + food bar at 23,400 mg/kg (P4); and the group of mice induced with DEN given the food bar (P5). The observations conducted included the spleen, which was used for the analysis of immunomodulators on T CD4+CD25+, CD4+, and CD8+ cells with cytokines TNF-α, IFN-Ɣ, and IL-10, blood serum for the analysis of SGPT and SGOT, and liver organ for the analysis of SOD, MDA, and histopathology. The results of the immunomodulator testing showed that the administration of the food bar (P5) significantly (p < 0.05) resulted in the highest percentage of CD4+ T cells at 2.93 % and CD8+ T cells at 5.30 %, a lower percentage of CD8+ T cells expressing proinflammatory cytokines (TNF-α at 0.27 % and IFN-γ at 0.29 %), a higher percentage of CD4+ T cells expressing CD25+, and CD25+ T cells expressing anti-inflammatory cytokines (IL-10) at 2.83 %. This group also exhibited the lowest levels of SGPT and SGOT, a higher percentage of SOD, and a lower percentage of MDA compared to other groups. The food bar, based on the mix of purple sweet potato, mung beans, moringa leaves, and strawberries, also reduced liver damage, as demonstrated through histopathological testing.

HIGHLIGHTS

Food bar demonstrated effectiveness as an immunomodulator by increasing CD4+ and CD8+ T-cell populations, enhancing CD4+ T-cells expressing CD25+, elevating CD25+ expressing anti-inflammatory cytokine (IL-10), and reducing CD8+ T-cells suppressing the expression of proinflammatory cytokines (TNF-α and IFN-γ) in the spleens of DEN-induced mice
The provision of the food bar as the main diet resulted in reduced levels of SGPT, SGOT, and hepatic MDA in DEN-induced mice and increased SOD activity in mice induced with 50 mg/kg of DEN
Food bar based on purple sweet potato, green beans, moringa leaves, and strawberries was able to protect and reduce liver cell damage caused by DEN, as demonstrated by histopathology tests

GRAPHICAL ABSTRACT

Physicochemical and Fatty Acid Profile of Refined Tuna Fish Oil By-Product from Canning and Meal Fish Industries

I Gusti Ayu Budiadnyani — 2024-02-25

Fish oil by-product (FOB) recovered from the precooking process in the cannery industry and the pressing process in fish meal production are potential resources. However, this fish oil still needs a refinery process to improve the quality of fish oil. This study aimed to identify the physicochemical characteristics of the initial fish oil by-product, the physicochemical profiles, and fatty acids of the fish oil by-product after degumming neutralization and bleaching (bentonite, zeolite, and carbon adsorbents) processes from the cannery and fish meal industry. Results showed the initial physicochemical characteristics of fish oil waste from fish flour have lower quality than canned products (acid value, free fatty acids, PV, AV, TOTOX value, viscosity, and refractive index) but are inversely proportional to the iodine value. Results showed degumming (D) and neutralization (N) processes from the Fish oil canning process (FCP) and Fish oil meal process (FMP) could improve the quality parameters of fish oil by-products by reducing the acid value (7.90, 0.65, 9.17, 0.58 mg KOH/g), respectively; free fatty acid (3.97, 0.33, 4.60, 0.29 % oleic) respectively; peroxide value (PV) (15.18, 7.38, 17.64, 7.10 mEq/kg), respectively; anisidine value (AV) (14.36, 6.61, 14.64, 6.51) respectively; total oxidation (TOTOX) value (44.72, 21.37, 49.64, 20.71) respectively; and iodine (167, 153, 163, 155 mg/100 g), respectively as well as increasing the lightness (23.80, 25.70, 20.65, 26.00), respectively. In the bleaching process (B), zeolite (15 %) was an effective adsorbent to obtain oil with the best quality parameters due to zeolite possessed greater polarity and surface area at a higher concentration, which resulted in the acid value for FCP and FMP (0.26, 0.22 mg KOH/g); free fatty acid (0.13, 0.15 % oleic); PV (2.44, 5.71 mEq/kg); AV (2.5, 4.9); TOTOX value (7.16, 9.11); and iodine (138, 151 mg/100 g) as well as increasing the lightness (37.08, 36.74) that are appropriate to International Fishmeal & Oil Manufacturers Association (IFOMA), Codex Alimentarius Committee (CAC), and International Fish Oils Standards (IFOS) standards (p < 0.05). All adsorbents effectively increase the brightness of the FOB. The higher the concentration of adsorbents, the higher the clarity of the FOB. For zeolite absorbents, there was an increase of 16.73 - 32.3 % for FCP and 15.39 - 32.69 % for FMP, but the viscosity decreased with a range of 24.50 - 36.50 cPs. FCP contains 14 fatty acids, while FMP contains 12 fatty acids. The number of SFA and MUFA detected in FCP is lower than in FMP. In contrast, the amount of PUFA in FCP is relatively higher than in FMP. The percentage of EPA and DHA in FCP is lower than in FMP. Overall, the refining process affects the fatty acid composition of fish oil by-products both in the canning process and in fish meal.

HIGHLIGHTS

Fish oil by-product (FOB) of yellowfin tuna (Thunnus albacares) from the fish canning process (CP), and fish meal process (MP) streams were successfully purified through a chemical process which was by IFOMA, CAC, and IFOSH standards
Zeolite resulted in the best fish oil quality at higher concentrations
The Final Fish oil canning process (FCP) was mainly composed of linoleic, whereas the Fish oil meal process (FMP) was highly distributed in oleic and palmitic acid
The sum of PUFA in FCP after bleaching was relatively higher than in FMP

GRAPHICAL ABSTRACT

Secondary Metabolites from Symbiotic Bacteria of Seagrass and Molluscs as A Reference for Natural Food Preservatives

Delianis Pringgenies — 2024-03-05

Symbiotic bacteria from seagrass and molluscs have the potential to be a source of secondary metabolites. Compounds from these secondary metabolites, such as natural food preservatives, can be used in the bioindustry sector. This research aims to identify the potential secondary metabolites from symbiotic bacteria in molluscs and seagrass that can support the Sustainable Development Goals (SDG) public health program. The samples used in this study were symbiotic bacteria of mollusc from seagrass environments, symbiotic bacteria from seagrass, and seagrass samples. The seagrass samples were analysed phytochemically, while the symbiotic bacteria samples from molluscs and seagrass were tested for their antibacterial activity against food spoilage pathogens such as Staphylococcus aureus and Escherichia coli using the disc diffusion method. Bacteria that showed potential antibacterial activity were identified using molecular methods, and the content of active compounds was analysed using gas chromatography-mass spectrometry. The phytochemical analysis revealed that seagrass contains flavones, tannins, steroids, and compounds with potential antibacterial properties. GC-MS analysis indicated that seagrass is predominantly composed of unsaturated fatty acids, which have antimicrobial effects by damaging bacterial cell membranes and disrupting the function of enzymes essential for growth and reproduction. Ten symbiotic bacteria isolates demonstrated proteolytic, cellulolytic, and lipolytic enzyme activity, suggesting their potential as antibacterial agents due to their ability to break down cell walls. Molecular identification of selected bacteria revealed the presence of mollusc symbionts, including Vibrio owensii, Bacillus paramycoides, and Pseudoalteromonas flavipulchra. The symbiotic bacteria found in seagrass in this study included Bacillus velezensis, Bacillus paramycoides, Nocardiopsis alba, Nocardiopsis alba, Bacillus tropicus, Lysinibacillus fusiformis, and Bacillus tropicus. GC-MS analysis of selected symbiotic bacteria from seagrass showed that the dominant compound components were phthalic acid, bis(7-methyl octyl) ester (25.18, 43.84, 35.55 %), and trimethoxy-3-piperidyl-2,2'-binaphthalene-1,1',4,4'-tetrone (36.36, 47.71, 64.48 %). In conclusion, seagrass and its symbiotic bacteria, as well as symbiotic bacteria from molluscs living in seagrass, have the potential to serve as antibacterial agents and can be used as references for food preservatives.

HIGHLIGHTS

Seagrass and mollusc symbiotic bacteria produce secondary metabolites that have antimicrobial properties, making them suitable for food preservation
The component that acts as an antibacterial agent is found in seagrass: Essential fatty acids, which can have antibacterial properties
Symbiotic bacteria are microorganisms that live in mutualistic symbiosis with other organisms.
The enzymatic activities of symbiotic bacteria can involve the production of enzymes that break down complex molecules into simpler molecules
In food preservation, symbiotic bacteria, with their enzymatic activity, can be used as natural preservatives
Using natural food preservatives derived from seagrass and mollusc symbiotic bacteria can contribute to a more sustainable and environmentally friendly approach to food preservation

GRAPHICAL ABSTRACT

Anti-Inflammatory Effects of Moringa Oleifera Lam Leaf Extract in Lipopolysaccharide-Activated Microglia

Anusorn Thampithak — 2024-03-10

Moringa oleifera Lam has been used as a medicinal plant in many Asian countries including Thailand. The plant has a variety of pharmacological effects such as antioxidant, anti-inflammation, hypolipidemic, anti-infection, anticancer, and antidiabetic. The leaves and young pods, which contain various nutritional compounds including proteins, fatty acids, and vitamins, are widely consumed. In this study, we investigated the effects of hexane, ethyl acetate, methanol and ethanol crude extracts of M. oleifera Lam leaves on the viability of microglial cells and on the lipopolysaccharide-induced microglial activation. which is widely used as a model for neuroinflammation. Our findings suggested that all of the crude extracts at concentrations ranging from 10^–9 to 10^–5 g/mL did not significantly affect the viability of microglial cells and exhibited similar effects. Subsequently, we focused on ethanol extract for investigation of the molecular mechanism involving neuroinflammation. We found that the ethanol extract of M. oleifera Lam leaves at the concentrations of 10^–9 to 10^–5 g/mL significantly reduced inducible nitric oxide synthase (iNOS) protein expression and nitric oxide (NO) production, accompanied by the reduction of phospho-NF-κB and phospho-IκBα expressions. The results suggested that the ethanol extract of M. oleifera Lam leaves reduced NO production through reduction of iNOS protein expression following the inhibition of NF-κB/IκBα signaling pathways. Our study underscored the therapeutic potential of M. oleifera Lam leaves extract in neurodegenerative diseases associated with neuroinflammation.

HIGHLIGHTS

The ethanol extract of M. oleifera Lam leaves dose-dependently reduced NO production in LPS-activated microglia
The reduction in NO production correlated with the reduction in iNOS protein expression
The extract inhibited the expression of NF-kB/I-kBα protein expression in a dose-dependent manner
The ethanol extract of oleifera Lam leaves exhibited anti-inflammatory effect via the inhibition of NF-kB/I-kBα signaling pathway

GRAPHICAL ABSTRACT

Irradiation Time Optimization on Photocatalytic Activity of Nanoparticles MgO from Dolomite Bangkalan

Lydia Rohmawati — 2024-02-25

Dolomite is a type of sedimentary rock material that is often found in the Bangkalan Madura area. This dolomite has a higher MgO compound content than in the other areas, namely 40 %, but its utilization has only been as fertilizer and building materials. For this reason, this research aims to determine the characteristics of MgO nanoparticles and their application in photocatalytic with visible light irradiation. The synthesis of MgO nanoparticles used the hydrochloric acid leaching method, and the results were characterized by X-ray diffraction, Fourier transform infrared, Scanning electron microscopy, Transmission electron microscopy, Raman spectroscopy, Photoluminescence, and Ultraviolet-visible spectroscopy. The results showed that the synthesized MgO nanoparticles had a periclase phase and were shaped like a spherical cube with an average nanoparticle size of 27 nm and an energy gap of 3.9 eV. The optical properties of the MgO nanoparticles showed quite strong luminescence at a Raman shift of 1,087.36 cm⁻¹, which was associated with the type of vibrational waves in the atomic lattice, and there were surface defects on the surface of the MgO nanoparticles, namely in the emission spectra of 720.06 and 740.39 nm originating from oxygen vacancies (F-center) and Mg vacancies (V-center). The photocatalytic activity of MgO nanoparticles in visible light showed the optimum time to degrade 30 ppm methylene blue dye in 360 min and yielded a degradation percentage of 99 %. Therefore, MgO nanoparticles could be used for processing industrial dye waste using visible light.

HIGHLIGHTS

The synthesis of MgO Nanoparticles from Bangkalan dolomite used the hydrochloric acid leaching method
The presence of nanocrystallite size, crystal defects, and oxygen vacancies in MgO nanoparticles can increase photocatalytic activity
MgO nanoparticles can degrade methylene blue dye with an optimum time of 360 minutes under visible light irradiation

GRAPHICAL ABSTRACT

Biocontrol of Fruit Discoloring and Fruit Rot Disease in Longan (Dimocarpus longan) by Using Antagonistic Bacillus spp.

Sakkarin Suksakol — 2024-02-25

Fruit discoloration and fruit rot disease in longan is a serious disease caused by the pathogenic fungi Pestalotiopsis oxyanthi and Lasiodiplodia pseudotheobromae. This study investigated the potential of antagonistic bacteria as an eco-friendly alternative to traditional fungicides for managing longan diseases caused by these fungal pathogens. Two bacterial isolates, UP-JLS050 and UP-JLS067, demonstrated significant inhibitory activity against the pathogenic fungi P. oxyanthi and L. pseudotheobromae, with high inhibitory activity. The bacterial strains were isolated from leaves and soil in a longan garden, and dual culture methods showed that the isolates UP-JLS050 and UP-JLS067 exhibited the highest inhibitory activity on the fungal pathogens, with percentage inhibitions of 72.67 ± 2.31 and 70.37 ± 1.28 %, respectively. Biochemical methods and molecular techniques, including sequencing of the 16S rDNA gene, were then used to identify the isolates. This confirmed their similarity to Bacillus subtilis and Bacillus amyloliquefaciens with 100 and 99 % identity, respectively. In vitro tests on longan fruit indicated that these antagonistic bacteria, administered at a concentration of 10⁸CFU/mL, significantly reduced disease incidence by up to 45 % after 9 days of incubation. The incubation of B. subtilis (UP-JLS050) and B. amyloliquefaciens (UP-JLS050) reduced disease incidence by 45 ± 5.77 and 32.5 ± 5.00 %, respectively.

HIGHLIGHTS

Fruit Discoloration and Rot in Longan: Caused by pathogenic fungi, namely Pestalotiopsis oxyanthi and Lasiodiplodia pseudotheobromae
Antagonistic Bacteria for Disease Control: This study explores the potential of antagonistic bacteria as an eco-friendly alternative to traditional fungicides for managing longan diseases caused by these fungal pathogens
Effective Inhibition: Two bacterial isolates, UP-JLS050 and UP-JLS067, demonstrated significant inhibitory activity against oxyanthi and L. pseudotheobromae, with inhibitions exceeding 70 % on PDA medium
Identification of Bacterial Strains: Molecular techniques, including 16S rDNA gene sequencing, confirmed the identity of these isolates as subtilis and B. amyloliquefaciens
Reduced Disease Incidence: In vitro tests on longan fruit indicated that these antagonistic bacteria, administered at a concentration of 10⁸ CFU/ml, significantly reduced disease incidence by up to 45 % after 9 days of incubation

GRAPHICAL ABSTRACT

The Effect of Fermentation Time, pH and Saccharomyces Cerevisiae Concentration for Bioethanol Production from Ulva Reticulata Macroalgae

Sefrinus Maria Dolfi Kolo — 2024-02-25

Research has been carried out on the effect of pH, fermentation time and yeast concentration on bioethanol production through hydrolysis using a CEM (Ceramic Elekctromagnetic Microwave) synthesizer and bioethanol production from Ulva reticulata seaweed. Ulva reticulata seaweed contains carbohydrates in the form of heteropolysaccharides such as glucose, arabinose, ramnose and xylose which are very abundant and suitable for conversion into bioethanol because the people of Timor Island do not use them as food. The carbohydrate content of Ulva reticulata seaweed can be converted into hexose and pentose sugars (glucose, arabinose, ramnose and xylose) through hydrolysis using 3 types of acid catalysts, namely hydrochloric acid (HCl), sulfuric acid (H₂SO₄) and nitric acid (HNO₃). Fermentation was carried out with S. cerevisiae concentration variation of 6; 8; 10; 12 % (v/v) and fermentation time variation of 3; 5; 7; 9 days and a pH variation of 4; 4.5; 5; 5.5 at a temperature of 30 °C. Reducing sugar characterization used the Dinitrosalicylic acid (DNS) reagent, sample surface texture analysis was carried out using Scanning Electron Microscopy (SEM) and ethanol characterization used Gas Chromatography-Flame Ionization Detector (GC-FID). The results of surface texture analysis before and after hydrolysis experienced significant changes. Optimal conditions for hydrolysis of Ulva reticulata seaweed using sulfuric acid (H₂SO₄) combined with a CEM synthesizer at an acid concentration of 3 % (v/v) with irradiation power of 200 watts for 50 min at a temperature of 150 °C, produced reducing sugar of 97.06 g/L. The results of GC-FID analysis indicated that bioethanol concentration obtained at optimal conditions of pH 4.5 was 42.32 %, S. cerevisiae concentration was 12 % with a bioethanol content of 42.53 % at a fermentation time of 5 days. This research is expected to provide information for researchers and industry to overcome world energy problems and environmental pollution through Ulva reticulata waste.

HIGHLIGHTS

The results of surface texture analysis before and after hydrolysis experienced significant changes. Optimum conditions for hydrolysis of Ulva reticulata seaweed using sulfuric acid (H₂SO₄) combined with a CEM synthesizer at an acid concentration of 3 % (v/v) with irradiation power of 200 watts for 50 min at a temperature of 150 °C, producing reducing sugar of 97.06 g /L.

GRAPHICAL ABSTRACT

Biofilm Production and Porin Permeability Activity in Clinical Isolates of Ciprofloxacin-Resistant Klebsiella pneumoniae in the Tertiary Hospital in Purwokerto, Indonesia

Indah Sulistiyawati — 2024-03-20

World Health Organization reports that the current use of ciprofloxacin as a broad-spectrum antibiotic shows a pattern of high bacterial resistance in many countries. Klebsiella pneumoniae is known as a Gram-negative bacterium that most often causes infection and is resistant to antibiotics. This study aimed to find patterns of resistance mechanisms based on biofilm production and porin permeability activity in K. pneumoniae clinical specimens from patients at RSUD Prof. Dr Margono Soekardjo Purwokerto, Indonesia. Several isolates of ciprofloxacin-resistant K. pneumoniae were isolated from clinical specimens of blood, sputum, urine, pus, stool and pleural fluid from August to October 2022. Identification and sensitivity testing of K. pneumoniae to ciprofloxacin were performed using Vitek® 2 Compact. A test for biofilm production is carried out by measuring optical density (OD) with a microplate at a wavelength of 630 nm. The porin permeability activity was determined using the value of the minimum inhibitory concentration (MIC) by the LC-MS spectrophotometry method at a wavelength of 630 nm. The results showed that 72 (47 %) isolates were resistant to ciprofloxacin. Among them, 41.3 % (24/58) of ciprofloxacin-resistant K. pneumoniae were able to produce strong biofilm. Porin permeability is indicated by MIC values of 1, 2 and 4 mg/L with bacterial cell counts of 32×10⁶, 19×10⁶ and 34×10⁶ CFU/mL, respectively. This number decreased from the initial control with a bacterial cell count of 10.15×10⁷ CFU/mL. Analysis of the correlation between the significance of biofilm production and the number of bacterial cells indicated that biofilm formation was related to the number of bacterial cells (R = 0.628, p ≤ 0.05). In conclusion, the resistance mechanism of ciprofloxacin-resistant K. pneumoniae involves biofilm production and decreased porin permeability activity.

HIGHLIGHTS

Major resistance mechanisms in pneumoniae decreased cell permeability from porin activity and biofilm formation
Bacterial outer membrane permeability to antimicrobial substances influences inherent resistance. Bacteria produce biofilm to increase their resistance to antibiotics. Porins are non-specific diffusion transport proteins that play a part in the antibiotic resistance process
The evolutionary development in the case of antibiotic resistance has an impact on the emergence of understanding in new perspective patterns on how to overcome health problems related to antibiotic resistance

GRAPHICAL ABSTRACT

Determination of the Betacyanin and Betaxanthin Contents of Red Beet (Beta Vulgaris) Powder Using Partial Least Square Regression Based on Visible-Near Infrared Spectra

Rudiati Evi Masithoh — 2024-03-15

Red beet (Beta vulgaris) contains betalain, which comprises red-violet betacyanin and yellow betaxanthin with esthetic and health benefits. Betacyanin and betaxanthin are usually detected using common chemical analysis, which requires a long time, skilled analysts, and sample destruction. For fast and accurate measurement, this study utilized a portable low-cost Visible-Near Infrared Spectra (Vis-NIR) spectrometer at 350 - 1000 nm combined with partial least square regression to predict the betacyanin and betaxanthin contents of red beet powder. The best calibration models for betacyanin and betaxanthin had R²c of 0.89 and 0.919, respectively, and standard error of calibration SEC of 0.108 and 0.037 mg/g, respectively. The models were able to predict the contents of both pigments with R²p of 0.87, standard error of prediction SEP of 0.108 mg/g, and the ratio of prediction to deviation RPD of 2.52 for betacyanin and R²p of 0.84, SEP of 0.056 mg/g, and RPD of 2.47 for betaxanthin. When applied to external unknown data, the models predicted the contents of betacyanin and betaxanthin with R² of 0.98 and root mean square error of 0.107 and 0.055 mg/g. Moreover, the predicted values were not significantly different at 95 % confidence.

HIGHLIGHTS

Betacyanin and betaxanthin from red beet, were determined nondestructively using a portable Vis-NIR spectrometer, an affordable device for small food industries
Betacyanin and betaxanthin regression coefficients showed peaks at 480 and 600 nm
Betacyanin regression coefficients peak was also observed at 540 nm

GRAPHICAL ABSTRACT

Studies on Nano-Indentation and Corrosion Behavior of Diamond-Like Carbon Coated Stainless Steel (316L)

Suresh Kolanji — 2024-03-20

Stainless steel 316L is commonly used in biomedical applications like dental implants, surgical tools, and other devices due to its corrosion resistance and cost-effective materials compared to other biomaterials. It must be enhanced since 316L SS has a relatively low hardness and wear resistance. The coating process includes applying functional materials to the surface to modify and give it the desired properties. This study used a PVD technique for coating diamond-like carbon (DLC) on the surface of 316L stainless steel to enhance its material surface properties. The coated and uncoated SS316 surfaces were exposed to nanoindentation (ultra-nano hardness tester (UNHT3) and electrochemical corrosion tests such as open circuit potential, potentiodynamic polarization, and electrochemical impedance spectroscopy in simulated body fluids at 37 °C. SEM characterized the morphologies and compositions of the surfaces with energy-dispersive X-ray spectroscopy.

HIGHLIGHTS

DLC coating on SS 316 using Physical Vapor Depositions process was presented
The nano indentation was employed for finding mechanical properties of coated and uncoated sample
The corrosion behaviors of coated and uncoated sample were performed. The surface topography of SS316 analyzed using SEM

GRAPHICAL ABSTRACT

Molecular Docking Investigation of Dioscorea alata Compounds Binding to CCKBR, CHRM3, CHRM5, and H2R for Gastric Ulcer Treatment

Sri Nabawiyati Nurul Makiyah — 2023-03-10

Gastric ulcer is a pathological condition characterized by damage to the mucosal and submucosal tissue layers of the stomach. The main proteins targeted in the treatment of gastric ulcers are CCKBR, CHRM3, CHRM5, and H2R because of their roles in the regulation of gastric acid secretion. Dioscorea alata (DA) is known to contain various active compounds with high sapogenin, diosgenin, and dioscorin content, in addition to its carbohydrate content. We investigated the potential of DA compounds to bind to these proteins in silico. Compound and protein structures were retrieved from PubChem (https://pubchem.ncbi.nlm.nih.gov), ChemSpider (http://www.chemspider.com), and the RCSB PDB (https://rcsb.org). Proteins and compounds are prepared first, and then docked to obtain the interactions that might form, and finally visualized to observe the interactions that have been constructed. The results showed that diosgenin and prosapogenin have potential as inhibitors of CCKBR and CHRM5, and diosgenin and dimethyl batatasin IV have potential as inhibitors of CHRM3 and H2R. We conclude that diosgenin, prosapogenin, and dimethyl batatasin IV in DA compounds have the potential to inhibit CCKBR, CHRM3, CHRM5, and H2R for gastric ulcer treatment. It is necessary to further study the effect of Dioscorea alata in vitro through a gastric ulcer model study.

HIGHLIGHTS

Dioscorea alata (DA) is known to contain various active compounds with high sapogenin, diosgenin, and dioscorin content in addition to its carbohydrate content
Gastritis (gastric ulcer) is a pathological condition characterized by damage to the mucosal and sub-mucosal tissue layers of the stomach. The main proteins targeted in the treatment of gastric ulcers are CCKBR, CHRM3, CHRM5, and H2R because of their roles in the regulation of gastric acid secretion
We performed an in-silico study to investigate the potential of DA compounds to inhibit these proteins. Compound and protein structures were retrieved from PubChem, ChemSpider, and the RCSB PDB. Proteins were prepared with Swiss PDB viewer v. 4.10. Compounds were prepared with Open Babel in Cytoscape v.3.9.1. Docking was done with AutoDock Vina in PyRx v.0.9.7. Visualization was done with Discovery Studio v. 19.1
We determined that several DA compounds have the potential to act as inhibitors of CCKBR, CHRM3, CHRM5, and H2R by reviewing the predicted binding energy, competitive properties based on literature studies and the bioactivity and molecular docking analysis. The results of this study are expected to be the basis for further gastric ulcer treatment research

GRAPHICAL ABSTRACT

Efficient Solutions with the LRPS Method for Non-Linear Fractional Order Tuberculosis Models

Samirah Hameed Alsulami — 2024-03-01

In this research article, we present a novel Non-Linear Fractional Order Tuberculosis mathematical model (NLFOTB) and introduce an efficient technique to obtain its solution. Fractional Order Models (FOMs) have garnered significant attention in contemporary research due to their widespread applicability. We address the challenge of solving the coupled Initial Value Problems (IVPs) associated with NLFOTB models by utilizing the groundbreaking LRPS method, which combines the RPS approach with the Laplace transform operator. This innovative approach generates approximate solutions in rapidly converging series forms, offering enhanced efficiency and reduced computational effort compared to conventional methods. Through the implementation of the LRPS method, we successfully derive an approximate solution for the NLFOTB model, contributing significantly to the field. Furthermore, our proposed approach demonstrates its efficacy in accurately capturing the dynamics of Tuberculosis (TB) through extensive computations and graphical representations, contributing to a deeper understanding of TB dynamics within a mathematical framework. Additionally, the LRPS method shows promise in tackling real world problems involving differential equations of various orders. Future investigations can extend the application of the LRPS method to explore other Fractional Order Models, further validating its effectiveness in a wide range of epidemic scenarios. Consequently, our study not only provides valuable insights into Tuberculosis dynamics but also introduces a powerful computational tool applicable to various practical problems in diverse disciplines, making a substantial contribution to the field of mathematical modeling and computation.

HIGHLIGHTS

· The comprehensive study highlights the LRPS strategy’s efficiency and accuracy in approximating solutions for fractional order equations. The research demonstrates its capability to predict compartmental behavior accurately within the specified range
· The discussions presented in the article significantly contribute to the field of epidemiology by introducing and showcasing the LRPS approach’s effectiveness. As a valuable tool for investigating and validating epidemic models, the LRPS method offers improved efficiency and convenience, thereby enhancing the understanding of disease dynamics
· The researchers anticipate that their findings will inspire further exploration and utilization of the LRPS technique in solving nonlinear models. This, in turn, is expected to contribute to advancements in the broader field of epidemiology, fostering continued innovation and development

GRAPHICAL ABSTRACT

The potential Two Types of Green Macroalgae (Caulerpa racemosa and Caulerpa lentillifera) as a Natural Food Preservative from Jepara beach, Indonesia

Gunawan Widi Santosa — 2024-03-05

Green macroalgae, known locally as Latoh, is one of the green seaweeds consumed by the local community in Jepara and is beneficial for health. This study explores the potential of secondary metabolites from seaweed and its symbiotic bacteria as natural food preservatives and antibacterials. Seaweed samples were collected from the seagrass ecosystem of Panjang Island, Jepara, Indonesia. Subsequently, the samples were subjected to scanning electron microscopy analysis, proximate analysis, phytochemical analysis, thin layer chromatography, and high-performance liquid chromatography for amino acid analysis. A sample was subjected to a multistage extraction process using n-hexane, ethyl acetate, and methanol (1:5 w/v), each for 24 h. Symbiotic bacteria from seaweed were isolated, and enzymatic (proteolytic, amylolytic, and cellulolytic) and antibacterial testing against pathogenic bacteria Staphylococcus aureus and Escherichia coli was conducted using the disc diffusion method. The selected bacteria were subjected to molecular identification. The research showed that Caulerpa lentillifera had an ash content of 3.24 %, protein content of 0.57 %, and fat content of 0.337 %. Phytochemical analysis shows that the sample contains flavonoids, steroids, and alkaloids. HPLC analysis reveals that Caulerpa lentillifera has the highest content of aspartic acid (relative area: 11.90 %), glutamic acid (relative area: 13.43 %), and alanine (relative area: 9.03 %). Caulerpa racemosa sample shows the highest detector response for glutamic acid (relative area: 12.19%), aspartic acid (relative area: 11.10 %), and alanine (relative area: 9.63 %). The results indicate that 14 bacterial isolates were successfully isolated, with 6 isolates from Caulerpa lentillifera and 8 isolates from Caulerpa racemosa, all exhibiting enzymatic and antibacterial abilities. The research results concluded that the Latoh seaweed species Caulerpa lentillifera and Caulerpa racemosa and their symbiotic bacteria have the potential to be used as food preservatives.

HIGHLIGHTS

HPLC analysis showed that Caulerpa lentillifera had the highest content of aspartic acid, glutamic acid, and alanine. Caulerpa racemosa also had high levels of glutamic acid, aspartic acid, and alanine
The research successfully isolated 14 bacterial isolates, with 6 isolates from Caulerpa lentillifera and eight isolates from Caulerpa racemosa, all exhibiting enzymatic and antibacterial abilities
Molecular identification revealed the presence of identified bacteria such as Pseudoalteromonas arabiensis, Pseudoalteromonas piscicida, Pseudoalteromonas shioyasakiensis, and Vibrio alginolyticus
seaweed species Caulerpa lentillifera and Caulerpa racemosa, along with their symbiotic bacteria, have the potential to be used as natural food preservatives due to their antibacterial and enzymatic pro perties

GRAPHICAL ABSTRACT

Synthesis, Spectroscopic Evaluations and UV-Vis Titration Studies of New Symmetrical Amide Compounds Derived from N-6-[(4-pyridylmethylamino)carbonyl]-pyridine-2-carboxylic Acid Methyl Ester

Maisara Abdul Kadir — 2024-02-25

In this study, 2 flexible and pre-organized tetraamide compounds derived from N-6-[(4-pyridylmethylamino)carbonyl]-pyridine-2-carboxylic acid methyl ester namely 1,2-bis[N,N’-6-(4-pyridylmethylamido)pyridyl-2-carboxyamido]pentane (L1) and 1,2-bis[N,N’-6-(4-pyridylmethylamido)pyridyl-2-carboxyamido]hexane (L2) have been successfully synthesized when reacted with diamines in 1:2 ratio. These new compounds were built from combination of 3 main components, as a trend requires for anion receptor which are (i) 2,6-pyridine dicarboxamide moieties as targeted anion binding host, (ii) amino methyl pyridine pendants arms as the flexible moieties and (iii) pentyl (-C₅H₁₀-) and hexyl (-C₆H₁₂-) unit as the spacer. Compounds L1-L2 were fully characterized by using elemental analyzer, Fourier transform infrared (FTIR) spectroscopy, gas chromatography-mass spectroscopy (GC-MS), ¹H, 2D NOESY and ¹³C Nuclear Magnetic Resonance (NMR) spectroscopies, and Ultraviolet-visible (UV-Vis) spectroscopies. In this study, anion titration methods were used to identify the affinity towards selected anions. The results showed that L1 (having a pentyl spacer) had the highest affinity towards phosphate anions as compared to L2, where the red shift changes were observed in the UV-vis spectrum at the amide region.

HIGHLIGHTS

Two novel amide compounds with flexible and pre-organized structure are designed for use as potential anion receptors
The flexible moieties at the amide and the linker allow hydrogen bonding interaction of the molecules with variety anions with different geometries
Anion titration studies revealed good affinities towards phosphate anions as suggested in Hofmeister trend

GRAPHICAL ABSTRACT

Anti-Diabetic Effect of Okara Noodles on Streptozotocin-Nicotinamide Induced Diabetic Rats

Kaisun Nesa Lesa — 2024-02-25

Okara is a by-product of soybean curd residue resulting from the processing of soy milk and tofu. Okara noodles were made from wheat flour (WF) and okara flour (OF) with a few modifications that consist of high dietary fiber. Therefore, okara can be considered as an effective functional component with health-promoting benefits, especially for diabetes mellitus. Albeit, the anti-diabetic effect has not been conscientiously investigated. The goal of the current research was to evaluate the anti-diabetic effect of okara noodles on Streptozotocin-Nicotinamide induced diabetes mellitus rats. In the experiment, the different ratios of OF was used to prepared 3 formulated noodles (F₀, F₁ and F₂) where F₀(0 % OF; 100 % WF), F₁ (25 % OF; 75 % WF) and F₂ (45 % OF; 55 % WF). Twenty-four male wistar rats were randomly divided into 4 groups (6 rats/groups), normal group of rats fed with standard feed and 3 diabetics groups, respectively fed with standard feed and formulated noodles variety (F₀, F₁ and F₂). In in vivo analysis, it indicated, diabetes mellitus rats fed okara noodles had lower blood glucose levels and HOMA-IR and higher serum insulin levels and HOMA-b index than those fed control noodles (F₀) as well as body weight managing activity. These outcomes demonstrated okara noodles possess significant anti-diabetic activity, prompting the applicability of okara noodles as potential food for the diabetes mellitus food products.

HIGHLIGHTS

Diabetes mellitus (DM) rats fed okara noodles had lower BGLs and HOMA-IR
DM rats fed okara noodles had higher serum insulin levels and HOMA-b index than those fed control noodles as well as body weight managing activity
Okara can be considered as an effective functional component for the anti-diabetic effect on STZ-NA induced DM rats

GRAPHICAL ABSTRACT

Enhancing Seed Germination and Seedlings Growth of Khao Dawk Mali 105 Rice Cultivar via Seed Coating and Infrared Drying Apparatus

Juckamas Laohavanich — 2024-02-25

Seed coating techniques had emerged as a promising approach for precise planting and safeguarding crops against environmental stresses. Simultaneously, infrared drying, renowned for its cost-effectiveness and efficiency, held promises for enhancing the quality of drying processes. This investigation delved into the procedures applied to Khao Dawk Mali 105 rice seeds during the coating and drying process using a prototype of the hexagonal drum. The results found that the drying temperature (factor B) and duration of drying time (factor C) had substantial primary effects (p ≤ 0.05), while the drum rotational speed (factor A) did not exert a noteworthy influence. Additionally, meaningful interactions were observed among the factors AB, AC, BC, and ABC. Impressively, it resulted in germination rates of 80.56 and 80.25 %, coupled with germination indices of 84.88 and 85.47 %, when utilizing a drum speed of 20 rpm, a drying temperature of 45 °C, and a drying time of 8 min (R-204508), and a drum speed of 10 rpm, a drying temperature of 45 °C, and a drying time of 8 min (R-104508), respectively. Additionally, the mean germination time amounted to 9.64 days, and the length of the rice seedling stem reached 13.66 cm when employing a drum speed of 20 rpm, a drying temperature of 50 °C, and a drying time of 8 min (R-205008). These discoveries emphasized the potential of the prototype's seed coating and infrared drying technology integration to improve rice germination and subsequent growth. This enhancement could ultimately result in higher yields and increased productivity, especially in small-scale agricultural settings.

HIGHLIGHTS

These discoveries emphasized the potential of the prototype's seed coating and infrared drying technology integration to improve rice germination and subsequent growth. This enhancement could ultimately result in higher yields and increased productivity, especially in small-scale agricultural settings.
Ensuring precise control over drying temperature and duration is of paramount importance in establishing optimal and consistent conditions for the germination and subsequent growth of rice seeds, thereby augmenting plant vigor and overall yield
The investigation determined optimal coating and drying conditions, encompassing a drum speed of 20 rpm, a drying temperature of 45 °C, and a drying duration of 8 min (treatment R-204508), along with a drum speed of 20 rpm, a drying temperature of 45 °C, and a drying time of 8 min (treatment R-104508). These conditions resulted in the highest mean germination rates and germination indices.

GRAPHICAL ABSTRACT

Physical and Chemical Characterization of Granules from 70 % Ethanol Extract of Ganitri Leaves (Eleocarpus serratus L.) using Wet Granulation Method as An Anti-Osteoporosis

Retno Widyowati — 2024-03-15

Elaeocarpus serratus L. leaves have been used as an antiosteoporosis treatment and it contains active compounds such as quercetin, rutin, and myricetin. The pre-formulation study was done by making granules from 70 % ethanol extract of this plant through the wet granulation method. These granules were determined by physical characteristics including organoleptic, flow properties, real and incompressible specific gravity, moisture, particle size distribution, and chemical characteristics including flavonoid content. The organoleptic result showed that all formulas had a strong characteristic odor and a slightly bitter taste. Formula A had a dark brown color, while formulas B and C were whitish brown. Based on the results of the flow properties test, all formulas had good flow properties because they had gone through the granulation process. The compressibility index, Hausner ratio, and % moisture content displayed that all formulas met the requirements and had almost the same value. In the assay process, the average % w/w content of formula A was 0.68 ± 0.017 %, formula B was 0.66 ± 0.044 %, and formula C was 0.16 ± 0.006 %. The most optimum formula was formula C with whitish brown color, slightly bitter taste, and strong odor, 10.90 ± 0.000 g/s of flow rate, 25.41 ± 0.000 ° of angle, 4.36 ± 0.214 % of moisture content, 13.80 % compressibility index, 1.1601 of Hausner ratio, 1772.04 µm of particle average distribution, and 0.66 ± 0.044 % w/w of percent routine flavonoid content.

HIGHLIGHTS

This study aims to determine the characteristics of granules from 70 % ethanol extract of ganitri leaves (Elaeocarpus serratus), the optimal formula to make granule preparations with active ingredients of ganitri leaves, and the levels of flavonoids using the spectrophotometer method. It also determined the characteristic physics of granule preparations including flow properties, organoleptic, moisture, particle size distribution, and real and incompressible specific gravity.

GRAPHICAL ABSTRACT

The Combination of Hvsr and Masw Methods with Landsat 8 Imagery to Assess the Changing Shoreline along the Coastal Area of Central Bengkulu

Muhammad Farid — 2024-03-15

The Central Bengkulu Regency possesses a coastline measuring 21.8 km, situated in direct proximity to the Indian Ocean. The heightened wave and ocean current dynamics possess the capacity to induce detrimental effects in the form of coastal abrasion along the coastline. This study conducted an examination of the rate of shoreline changes resulting from coastal abrasion in Central Bengkulu Regency, Bengkulu, Indonesia. Horizontal-to-vertical spectral ratio (HVSR) analysis, multichannel analysis of surface waves (MASW), and Landsat-8 remote sensing were all used in the current investigation. The project gets started with conducting field investigations and measurements of geophysical techniques, including the collection of Landsat-8 satellite imagery spanning the period from 1990 to 2020. Additionally, an examination was conducted on the rate at which the shoreline undergoes changes in velocity. In order to determine the increase in change that occurs along coastlines over a period of several decades, a study was carried out during which Landsat-8 satellite images were analyzed. This study investigates the application of the HVSR technique for assessing the seismic vulnerability of the ground and, additionally, the MASW technique for measuring the shear wave velocity of the coastline’s soil. Both of these methodologies are compared and contrasted with one another in this research. The findings indicated that the mean pace of coastline change in Central Bengkulu Regency was 1.5 m/yr, with the maximum velocity recorded at 4.1 m/yr. This high velocity of shoreline change is correlated with the Vs30 value of MASW measurement and Kg of HVSR measurement, where the subsurface soil structure along the coast of Central Bengkulu Regency from Vs30 measurement shows that it is dominated by stiff soil structure that is susceptible to erosion. Outcomes from the study can inform decision-making processes about safeguards and preventative measures to prevent further coastal degradation.

HIGHLIGHTS

In this paper, we present a framework using application passive and active seismic method with Landsat 8 for knowing the changing of shoreline coastal area in Central Bengkulu Indonesia
This study demonstrated that the high velocity of the shifting shoreline is correlated with Vs30 and Kg values
The subsurface soil structure is predominantly composed of stiff soil, and the changing shoreline starts at 1.5 mm/yr, which makes the coast of Central Bengkulu vulnerable to erosion

GRAPHICAL ABSTRACT

Identification of CRISPR/Cas12a (Cpf1) guideRNA Sequence Targeting the Mitochondrial DNA D-loop Region in Wild Pig (Sus scrofa) Through Homology Difference and Mismatch Analysis

Muhammad Ihda Hamlu Liwaissunati Zein — 2024-03-20

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) or CRISPR-associated (Cas) System has become a major gene editing tool. Gene editing with CRISPR requires the Cas protein and the corresponding guide RNA (gRNA). However, low cleavage efficiency and off-target effects can impede the application of the CRISPR/Cas system. Therefore, the determination of specific gRNAs is essential. In biosensor applications, CRISPR/Cas12a can enhance specificity and sensitivity in identifying target genes due to the trans-cleavage activity of Cas12a (Cpf1). The mtDNA D-loop sequence is the most variable part of mtDNA, making it suitable for distinguishing between species. Consequently, the objective of this study was to determine the gRNA sequence of the D-loop of wild pig mtDNA in silico. Candidate gRNAs were predicted using the Benchling application with the assistance of the GenBank database. The gRNA candidates were subsequently subjected to a homology difference analysis using BLAST nucleotide and a mismatch test using Jalview. Among several candidates, candidate 1 was selected as the best option, with an Off-target value of 99.8. The homology difference analysis against competitors and the mismatch test against the Sus genus resulted in high E-values and high percentage values, respectively. This suggests that the candidate will not recognize other species but can detect members of the Sus scrofa species. These gRNA candidates can be selectively and sensitively applied to biosensors for the detection of meat adulteration.

HIGHLIGHTS

The D-loop exhibits high variability, make it useful for distinguishing between species
gRNA as an enabler of CRISPR/Cas12a to identify the target and initiate cleavage activity
The candidate 1 was selected with the sequence 5'-GAT TGT CGT GCC GGA TCA TGA GTT-3'
The application of this strategy is aimed at determining the halal status of food products
The application will encompass both quantitative and qualitative aspects

GRAPHICAL ABSTRACT

Purification of IgY Anti-SARS-CoV-2-Chicken Nucleocapsid and Elimination of Its Low-temperature Storage-induced Aggregates

Idar Idar — 2024-03-20

Even though the COVID-19 pandemic has ended, the virus is still circulating. It is important to detect it, as its symptoms are similar to other endemic diseases, e.g. dengue fever. Detection can be done using birds’ specific immunoglobulin Y, IgY Anti-SARS-CoV-2-Chicken Nucleocapsid (IgY anti-N), obtained by immunising chickens with the SARS CoV2 nucleocapsid protein. However, IgY purification requires further research, as commonly used ligands are unsuitable. Another common problem with IgY anti-N is aggregation, even when stored at low temperatures. Thus, this research aims to purify IgY anti-N and eliminate low-temperature storage-induced aggregates. In this study, polyethene glycol (PEG) was used for delipidation. The resulting IgY was divided into 2 parts, one of which was stored at 4 °C and purified within the next 1 or 2 days (labelled as IgY 4 °C), while the other was stored at −20 °C and purified within a period of 1 or 2 years (labelled as IgY −20 °C). The next step involved purifying both samples using hydrophobic chromatography and sequential anion exchange. Peaks resulting from the hydrophobic column were analyzed using High-Performance Liquid Chromatography (HPLC) and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Another purification result was analysed using PAGE. The migration of each separation step result was observed using native, denaturing and non-reducing PAGE. The study showed, qualitatively, that using two-step chromatographic techniques, hydrophobic interaction and ion exchange chromatography significantly increased the purity of IgY during purification. Additionally, the results demonstrated that hydrophobic chromatography purifies IgY and removes aggregates that may arise from storage. Moreover, similar to immunoglobulin G, IgY anti-N was found to have an uncommon migration in native PAGE compared to other proteins.

HIGHLIGHTS

PEG precipitation for delipidation of egg yolk containing IgY
Two-step chromatographic method for IgY purification: hydrophobic interaction and ion exchange
Hydrophobic interaction chromatography can purify and also remove the aggregate of the IgY sample
Native PAGE cannot be used to calculate antibody molecular weight

GRAPHICAL ABSTRACT

The Effect of Time of Day on Lumbar Repositioning Sense Variability in Asymptomatic Participants with Seated Sedentary Behavior Over Two Consecutive Days

Pongsatorn Saiklang — 2024-03-20

Prolonged sitting is associated with sedentary behavior and lumbar repositioning error (LRPE), a factor linked to low back pain (LBP). The measurement of LRPE is commonly used to assess the effectiveness of interventions in preventing LBP. However, the impact of time of day and day-to-day variability on LRPE measurements during a sitting condition remains unclear. The primary aim of the current study is to investigate whether the variability of LRPE in the sitting condition is influenced by the time of day and 2 consecutive days among asymptomatic participants. Fifty asymptomatic participants were enrolled, and LRPE measurements were taken before and after a 30-minute sitting condition conducted in the morning and afternoon over 2 consecutive days. The findings revealed no significant difference in LRPE magnitude between morning and afternoon sessions. However, a slight increase in variability was observed in the afternoon (initial sitting: 1.21 cm, post-sitting: 1.27 cm) compared to the morning (initial sitting: 1.10 cm, post-sitting: 1.16 cm). These findings suggest that future LRPE investigations can be conducted either in the morning or afternoon. However, it is crucial to control for measurement times due to the influence of variability on LRPE outcomes. The study also demonstrated specific levels of natural variation in LRPE measures across different days and times of the day. Any changes beyond the reported results can potentially be attributed to the effects of the intervention.

HIGHLIGHTS

A meaningful response should exceed the values of 1.10 cm (initial sitting) and 1.16 cm (after the 30-minute sitting) in the morning, and 1.21 cm (initial sitting) and 1.27 cm (after the 30-minute sitting) in the afternoon between consecutive days
Controlling measurement time is crucial in lumbar repositioning error investigations to mitigate the impact of variability on outcomes
This information guides future investigations and establishes meaningful benchmarks for assessing intervention effectiveness

GRAPHICAL ABSTRACT